Thrombin generation in different commercial sodium citrate blood tubes
Background: This study aimed to verify whether blood drawn into six different commercial coagulation tubes generated comparable results of thrombin generation. Methods: Blood was sequentially collected from 20 healthy subjects into different brand and draw volume 3.2% sodium citrate tubes (4.3 mL Sa...
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| Format: | Article |
| Language: | English |
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Society of Medical Biochemists of Serbia, Belgrade
2020-01-01
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| Series: | Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2020/1452-82582001019L.pdf |
| _version_ | 1828259413342814208 |
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| author | Luca Salvagno Gian Demonte Davide Gelati Matteo Poli Giovanni Favaloro Emmanuel J. Lippi Giuseppe |
| author_facet | Luca Salvagno Gian Demonte Davide Gelati Matteo Poli Giovanni Favaloro Emmanuel J. Lippi Giuseppe |
| author_sort | Luca Salvagno Gian |
| collection | DOAJ |
| description | Background: This study aimed to verify whether blood drawn into six different commercial coagulation tubes generated comparable results of thrombin generation. Methods: Blood was sequentially collected from 20 healthy subjects into different brand and draw volume 3.2% sodium citrate tubes (4.3 mL Sarstedt, 3.0 mL Greiner, 2.7 mL Becton Dickinson, 2.0 mL Kima, 1.8 mL Sarstedt and 1.0 mL Greiner). Thrombin generation was measured in plasma with the fully-automated ST Genesia analyzer using the weakest trigger (STG-BleedScreen). Results: Different values of lag time (LT), time to reach thrombin peak (TP), thrombin peak height (PH) and endogenous thrombin potential (ETP) were commonly found in different tubes. Thrombin generation was the lowest in 4.3 mL Sarstedt tubes and the highest in 1.0 mL Greiner tubes. Other tubes displayed intermediate values. In multiple comparisons, LT was significantly different in 6/15 cases (40%), whilst PH, TP and ETP were significantly different in 14/15 (93%), 13/15 (87%) and 13/15 (87%) cases. The mean percent bias of LT, PH, TP and ETP ranged between -6% and +1%, -27% and +116%, -22% and +8%, and between -18% and +65%. The intra-assay imprecision of LT, PH, TP and ETP was exceeded in 0/15 (0%), 13/15 (87%), 6/15 (40%) and 13/15 (87%) comparisons. The correlation of LT, PH, TP and ETP values in different tubes ranged between 0.718-0.971, 0.570-0.966, 0.725-0.977 and 0.101-0.904. Conclusions: Blood collection for thrombin generation assays requires local standardization using identical tubes for brand and draw volume, and reference ranges calculated according to type of tubes. |
| first_indexed | 2024-04-13T03:07:16Z |
| format | Article |
| id | doaj.art-34da0d33459442738586c455f4fc6d1c |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-04-13T03:07:16Z |
| publishDate | 2020-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-34da0d33459442738586c455f4fc6d1c2022-12-22T03:05:11ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662020-01-0139119241452-82582001019LThrombin generation in different commercial sodium citrate blood tubesLuca Salvagno Gian0Demonte Davide1Gelati Matteo2Poli Giovanni3Favaloro Emmanuel J.4Lippi Giuseppe5University of Verona, Section of Clinical Biochemistry, Verona, ItalyUniversity of Verona, Section of Clinical Biochemistry, Verona, ItalyUniversity of Verona, Section of Clinical Biochemistry, Verona, ItalyUniversity of Verona, Section of Clinical Biochemistry, Verona, ItalyNSW Health Pathology, Westmead Hospital, Institute of Clinical Pathology and Medical Research, Sydney Centres for Thrombosis and Haemostasis, Department of Haematology, Westmead, NSW, AustraliaUniversity of Verona, Section of Clinical Biochemistry, Verona, ItalyBackground: This study aimed to verify whether blood drawn into six different commercial coagulation tubes generated comparable results of thrombin generation. Methods: Blood was sequentially collected from 20 healthy subjects into different brand and draw volume 3.2% sodium citrate tubes (4.3 mL Sarstedt, 3.0 mL Greiner, 2.7 mL Becton Dickinson, 2.0 mL Kima, 1.8 mL Sarstedt and 1.0 mL Greiner). Thrombin generation was measured in plasma with the fully-automated ST Genesia analyzer using the weakest trigger (STG-BleedScreen). Results: Different values of lag time (LT), time to reach thrombin peak (TP), thrombin peak height (PH) and endogenous thrombin potential (ETP) were commonly found in different tubes. Thrombin generation was the lowest in 4.3 mL Sarstedt tubes and the highest in 1.0 mL Greiner tubes. Other tubes displayed intermediate values. In multiple comparisons, LT was significantly different in 6/15 cases (40%), whilst PH, TP and ETP were significantly different in 14/15 (93%), 13/15 (87%) and 13/15 (87%) cases. The mean percent bias of LT, PH, TP and ETP ranged between -6% and +1%, -27% and +116%, -22% and +8%, and between -18% and +65%. The intra-assay imprecision of LT, PH, TP and ETP was exceeded in 0/15 (0%), 13/15 (87%), 6/15 (40%) and 13/15 (87%) comparisons. The correlation of LT, PH, TP and ETP values in different tubes ranged between 0.718-0.971, 0.570-0.966, 0.725-0.977 and 0.101-0.904. Conclusions: Blood collection for thrombin generation assays requires local standardization using identical tubes for brand and draw volume, and reference ranges calculated according to type of tubes.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2020/1452-82582001019L.pdfpreanalytical variabilityblood tubesblood collectionthrombin generation |
| spellingShingle | Luca Salvagno Gian Demonte Davide Gelati Matteo Poli Giovanni Favaloro Emmanuel J. Lippi Giuseppe Thrombin generation in different commercial sodium citrate blood tubes Journal of Medical Biochemistry preanalytical variability blood tubes blood collection thrombin generation |
| title | Thrombin generation in different commercial sodium citrate blood tubes |
| title_full | Thrombin generation in different commercial sodium citrate blood tubes |
| title_fullStr | Thrombin generation in different commercial sodium citrate blood tubes |
| title_full_unstemmed | Thrombin generation in different commercial sodium citrate blood tubes |
| title_short | Thrombin generation in different commercial sodium citrate blood tubes |
| title_sort | thrombin generation in different commercial sodium citrate blood tubes |
| topic | preanalytical variability blood tubes blood collection thrombin generation |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2020/1452-82582001019L.pdf |
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