Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients
Background & Objective: Breast cancer is the most common cancer among women worldwide. Cell-cycle is controlled by regulatory proteins called cyclins and Cyclin-Dependent Kinases. Aberrant activity of cyclins plays an important role in cancer development and progression. The Cyclin E gene, calle...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2020-08-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-2078-en.pdf |
| _version_ | 1797679544971296768 |
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| author | Goharik Torosian Mansour Salehi Massoud Houshmand |
| author_facet | Goharik Torosian Mansour Salehi Massoud Houshmand |
| author_sort | Goharik Torosian |
| collection | DOAJ |
| description | Background & Objective: Breast cancer is the most common cancer among women worldwide. Cell-cycle is controlled by regulatory proteins called cyclins and Cyclin-Dependent Kinases. Aberrant activity of cyclins plays an important role in cancer development and progression. The Cyclin E gene, called CCNE1 or pCCNE1, is one of the four cyclins groups of the cell cycle, reported in a variety of cancers as well as breast cancer, and is a notice target in cancer research. In this regard, Cyclin E gene amplification in breast cancer patients was evaluated and investigated and its capacity as a molecular biomarker was assessed.
Materials & Methods: Tumor tissue samples as well as adjacent healthy tissues, were excised and collected from breast cancer patients undergoing surgery in Al-Zahra hospital. Genomic DNA was extracted using phenol-chloroform method. The extracted products concentration was normalized and applied in Real-time PCR technique. Amplification of aforementioned gene was determined by calculating Cts using 2-∆∆Ct method and was evaluated by Wilcoxon statistical test.
Result: The Cyclin E gene amplification in breast cancer tissue was significantly higher than healthy tissue (p = 0.002). Furthermore, the amplification of Cyclin E gene was investigated as a breast cancer biomarker by the ROC curve statistical analysis.
Conclusion: The results revealed, the area under the ROC curve (AUC) was close in 70 percent. As a result, the Cyclin E gene amplification in neoplasm dramatically increases and can be used as a biomarker for the diagnosis and prognosis of breast cancer. |
| first_indexed | 2024-03-11T23:16:20Z |
| format | Article |
| id | doaj.art-34da8c275cf64f1b8e0e310832276f6b |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:16:20Z |
| publishDate | 2020-08-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-34da8c275cf64f1b8e0e310832276f6b2023-09-20T21:27:39ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232020-08-0110326252635Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer PatientsGoharik Torosian0Mansour Salehi1Massoud Houshmand2 Nouredanesh Institute of Higher Education, Meymeh, Iran Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran Genetic Diagnosis Lab, Mendel Genetic Center, Erbil, Iraq Background & Objective: Breast cancer is the most common cancer among women worldwide. Cell-cycle is controlled by regulatory proteins called cyclins and Cyclin-Dependent Kinases. Aberrant activity of cyclins plays an important role in cancer development and progression. The Cyclin E gene, called CCNE1 or pCCNE1, is one of the four cyclins groups of the cell cycle, reported in a variety of cancers as well as breast cancer, and is a notice target in cancer research. In this regard, Cyclin E gene amplification in breast cancer patients was evaluated and investigated and its capacity as a molecular biomarker was assessed. Materials & Methods: Tumor tissue samples as well as adjacent healthy tissues, were excised and collected from breast cancer patients undergoing surgery in Al-Zahra hospital. Genomic DNA was extracted using phenol-chloroform method. The extracted products concentration was normalized and applied in Real-time PCR technique. Amplification of aforementioned gene was determined by calculating Cts using 2-∆∆Ct method and was evaluated by Wilcoxon statistical test. Result: The Cyclin E gene amplification in breast cancer tissue was significantly higher than healthy tissue (p = 0.002). Furthermore, the amplification of Cyclin E gene was investigated as a breast cancer biomarker by the ROC curve statistical analysis. Conclusion: The results revealed, the area under the ROC curve (AUC) was close in 70 percent. As a result, the Cyclin E gene amplification in neoplasm dramatically increases and can be used as a biomarker for the diagnosis and prognosis of breast cancer.http://jabs.fums.ac.ir/article-1-2078-en.pdfbreast cancercyclin ereal-time pcrgene amplificationbiomarker |
| spellingShingle | Goharik Torosian Mansour Salehi Massoud Houshmand Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients Journal of Advanced Biomedical Sciences breast cancer cyclin e real-time pcr gene amplification biomarker |
| title | Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients |
| title_full | Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients |
| title_fullStr | Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients |
| title_full_unstemmed | Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients |
| title_short | Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients |
| title_sort | assessment of cyclin e gene amplification as a biomarker in isfahan province breast cancer patients |
| topic | breast cancer cyclin e real-time pcr gene amplification biomarker |
| url | http://jabs.fums.ac.ir/article-1-2078-en.pdf |
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