Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family from Citrus sinensis in response to huanglongbing
IntroductionHeavy metal-associated isoprenylated plant proteins (HIPPs) play vital roles in maintaining heavy metal balance and responding to both biotic and abiotic stresses in vascular plants. However, the role of HIPPs in the response to Huanglongbing (HLB), a harmful disease of citrus caused by...
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1369883/full |
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| author | Guiyan Huang Guiyan Huang Yanan Hu Fuxuan Li Xiru Zuo Xinyou Wang Fengyao Li Ruimin Li Ruimin Li |
| author_facet | Guiyan Huang Guiyan Huang Yanan Hu Fuxuan Li Xiru Zuo Xinyou Wang Fengyao Li Ruimin Li Ruimin Li |
| author_sort | Guiyan Huang |
| collection | DOAJ |
| description | IntroductionHeavy metal-associated isoprenylated plant proteins (HIPPs) play vital roles in maintaining heavy metal balance and responding to both biotic and abiotic stresses in vascular plants. However, the role of HIPPs in the response to Huanglongbing (HLB), a harmful disease of citrus caused by the phloem-colonizing bacterium Candidatus Liberibacter asiaticus (CLas), has not been examined.Methods and resultsIn this study, a total of 26 HIPP genes were identified in Citrus sinensis, and they were grouped into 5 clades. The CsHIPP genes are distributed on 8 chromosomes and exhibited considerable synteny with HIPPs found in Arabidopsis thaliana. Additionally, we analyzed the gene structure, conserved motifs and domains of the CsHIPPs. Various cis-acting elements related to plant hormones and stress responses were identified in the promoters of CsHIPPs. Public transcriptome data and RT-qPCR analysis showed that the expression level of CsHIPP03 was significantly reduced in samples infected by CLas and Xanthomonas citri ssp. citri (Xcc). Furthermore, silencing the homologous gene of CsHIPP03 in Nicotiana benthamiana increased the disease resistance of plants to bacteria.DiscussionOur results provide a basis for functional studies of HIPP gene family in C. sinensis, highlighting their functions in bacterial resistance, and improve our understanding to the susceptibility mechanism of HLB. |
| first_indexed | 2024-04-24T18:46:07Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-24T18:46:07Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-34dc4cfdc40b4f9bbe7b74e585a0f4152024-03-27T05:10:39ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-03-011510.3389/fpls.2024.13698831369883Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family from Citrus sinensis in response to huanglongbingGuiyan Huang0Guiyan Huang1Yanan Hu2Fuxuan Li3Xiru Zuo4Xinyou Wang5Fengyao Li6Ruimin Li7Ruimin Li8College of Life Sciences, Gannan Normal University, Ganzhou, ChinaChina-USA Citrus Huanglongbing Joint Laboratory, National Navel Orange Engineering Research Center, College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi, ChinaCollege of Life Sciences, Gannan Normal University, Ganzhou, ChinaCollege of Life Sciences, Gannan Normal University, Ganzhou, ChinaCollege of Life Sciences, Gannan Normal University, Ganzhou, ChinaCollege of Life Sciences, Gannan Normal University, Ganzhou, ChinaCollege of Life Sciences, Gannan Normal University, Ganzhou, ChinaCollege of Life Sciences, Gannan Normal University, Ganzhou, ChinaChina-USA Citrus Huanglongbing Joint Laboratory, National Navel Orange Engineering Research Center, College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi, ChinaIntroductionHeavy metal-associated isoprenylated plant proteins (HIPPs) play vital roles in maintaining heavy metal balance and responding to both biotic and abiotic stresses in vascular plants. However, the role of HIPPs in the response to Huanglongbing (HLB), a harmful disease of citrus caused by the phloem-colonizing bacterium Candidatus Liberibacter asiaticus (CLas), has not been examined.Methods and resultsIn this study, a total of 26 HIPP genes were identified in Citrus sinensis, and they were grouped into 5 clades. The CsHIPP genes are distributed on 8 chromosomes and exhibited considerable synteny with HIPPs found in Arabidopsis thaliana. Additionally, we analyzed the gene structure, conserved motifs and domains of the CsHIPPs. Various cis-acting elements related to plant hormones and stress responses were identified in the promoters of CsHIPPs. Public transcriptome data and RT-qPCR analysis showed that the expression level of CsHIPP03 was significantly reduced in samples infected by CLas and Xanthomonas citri ssp. citri (Xcc). Furthermore, silencing the homologous gene of CsHIPP03 in Nicotiana benthamiana increased the disease resistance of plants to bacteria.DiscussionOur results provide a basis for functional studies of HIPP gene family in C. sinensis, highlighting their functions in bacterial resistance, and improve our understanding to the susceptibility mechanism of HLB.https://www.frontiersin.org/articles/10.3389/fpls.2024.1369883/fullCitrus sinensisheavy metal-associated isoprenylated plant proteinsphylogenetic analysissusceptible genehuanglongbing |
| spellingShingle | Guiyan Huang Guiyan Huang Yanan Hu Fuxuan Li Xiru Zuo Xinyou Wang Fengyao Li Ruimin Li Ruimin Li Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family from Citrus sinensis in response to huanglongbing Frontiers in Plant Science Citrus sinensis heavy metal-associated isoprenylated plant proteins phylogenetic analysis susceptible gene huanglongbing |
| title | Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family from Citrus sinensis in response to huanglongbing |
| title_full | Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family from Citrus sinensis in response to huanglongbing |
| title_fullStr | Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family from Citrus sinensis in response to huanglongbing |
| title_full_unstemmed | Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family from Citrus sinensis in response to huanglongbing |
| title_short | Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family from Citrus sinensis in response to huanglongbing |
| title_sort | genome wide characterization of heavy metal associated isoprenylated plant protein gene family from citrus sinensis in response to huanglongbing |
| topic | Citrus sinensis heavy metal-associated isoprenylated plant proteins phylogenetic analysis susceptible gene huanglongbing |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1369883/full |
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