Effects of Apigenin on Pharmacokinetics of Dasatinib and Probable Interaction Mechanism

Dasatinib (DAS), a narrow-therapeutic index drug, Bcr-Abl, and Src family kinases multitarget inhibitor have been approved for chronic myelogenous leukemia (CML) and Ph-positive acute lymphocytic leukemia (Ph+ ALL). Apigenin (APG) has a long history of human usage in food, herbs, health supplements,...

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Main Authors: Mohammad Raish, Ajaz Ahmad, Mudassar Shahid, Yousef A. Bin Jardan, Abdul Ahad, Mohd Abul Kalam, Mushtaq Ahmad Ansari, Muzaffar Iqbal, Naushad Ali, Khalid M. Alkharfy, Fahad I. Al-Jenoobi
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/28/4/1602
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author Mohammad Raish
Ajaz Ahmad
Mudassar Shahid
Yousef A. Bin Jardan
Abdul Ahad
Mohd Abul Kalam
Mushtaq Ahmad Ansari
Muzaffar Iqbal
Naushad Ali
Khalid M. Alkharfy
Fahad I. Al-Jenoobi
author_facet Mohammad Raish
Ajaz Ahmad
Mudassar Shahid
Yousef A. Bin Jardan
Abdul Ahad
Mohd Abul Kalam
Mushtaq Ahmad Ansari
Muzaffar Iqbal
Naushad Ali
Khalid M. Alkharfy
Fahad I. Al-Jenoobi
author_sort Mohammad Raish
collection DOAJ
description Dasatinib (DAS), a narrow-therapeutic index drug, Bcr-Abl, and Src family kinases multitarget inhibitor have been approved for chronic myelogenous leukemia (CML) and Ph-positive acute lymphocytic leukemia (Ph+ ALL). Apigenin (APG) has a long history of human usage in food, herbs, health supplements, and traditional medicine, and it poses low risk of damage. The concomitant use of APG containing herbs/foods and traditional medicine may alter the pharmacokinetics of DAS, that probably lead to possible herb–drug interactions. The pharmacokinetic interaction of APG pretreatment with DAS in rat plasma following single and co-oral dosing was successfully deliberated using the UPLC–MS/MS method. The in vivo pharmacokinetics and protein expression of CYP3A2, Pgp-MDR1, and BCPR/ABCG2 demonstrate that APG pretreatment has potential to drastically changed the DAS pharmacokinetics where escalation in the Cmax, AUC<sub>(0–t)</sub>, AUMC<sub>(0-inf_obs)</sub>, T<sub>1/2</sub>, Tmax, and MRT and reduction in K<i>el</i>, Vd, and Cl significantly in rats pretreated with APG 40 mg/kg, thus escalating systemic bioavailability and increasing the rate of absorption via modulation of CYP3A2, Pgp-MDR1, and BCPR/ABCG2 protein expression. Therefore, the concomitant consumption of APG containing food or traditional herb with DAS may cause serious life-threatening drug interactions and more systematic clinical study on herb–drug interactions is required, as well as adequate regulation in herbal safety and efficacy.
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spelling doaj.art-34e02e397d7542a8be7e7e80346539cb2023-11-16T22:20:43ZengMDPI AGMolecules1420-30492023-02-01284160210.3390/molecules28041602Effects of Apigenin on Pharmacokinetics of Dasatinib and Probable Interaction MechanismMohammad Raish0Ajaz Ahmad1Mudassar Shahid2Yousef A. Bin Jardan3Abdul Ahad4Mohd Abul Kalam5Mushtaq Ahmad Ansari6Muzaffar Iqbal7Naushad Ali8Khalid M. Alkharfy9Fahad I. Al-Jenoobi10Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaQuality Assurance Unit, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDasatinib (DAS), a narrow-therapeutic index drug, Bcr-Abl, and Src family kinases multitarget inhibitor have been approved for chronic myelogenous leukemia (CML) and Ph-positive acute lymphocytic leukemia (Ph+ ALL). Apigenin (APG) has a long history of human usage in food, herbs, health supplements, and traditional medicine, and it poses low risk of damage. The concomitant use of APG containing herbs/foods and traditional medicine may alter the pharmacokinetics of DAS, that probably lead to possible herb–drug interactions. The pharmacokinetic interaction of APG pretreatment with DAS in rat plasma following single and co-oral dosing was successfully deliberated using the UPLC–MS/MS method. The in vivo pharmacokinetics and protein expression of CYP3A2, Pgp-MDR1, and BCPR/ABCG2 demonstrate that APG pretreatment has potential to drastically changed the DAS pharmacokinetics where escalation in the Cmax, AUC<sub>(0–t)</sub>, AUMC<sub>(0-inf_obs)</sub>, T<sub>1/2</sub>, Tmax, and MRT and reduction in K<i>el</i>, Vd, and Cl significantly in rats pretreated with APG 40 mg/kg, thus escalating systemic bioavailability and increasing the rate of absorption via modulation of CYP3A2, Pgp-MDR1, and BCPR/ABCG2 protein expression. Therefore, the concomitant consumption of APG containing food or traditional herb with DAS may cause serious life-threatening drug interactions and more systematic clinical study on herb–drug interactions is required, as well as adequate regulation in herbal safety and efficacy.https://www.mdpi.com/1420-3049/28/4/1602dasatinibsinapic acidherb drug interactionsPgP/MDR1BCPR/ABCG2CYP3A2
spellingShingle Mohammad Raish
Ajaz Ahmad
Mudassar Shahid
Yousef A. Bin Jardan
Abdul Ahad
Mohd Abul Kalam
Mushtaq Ahmad Ansari
Muzaffar Iqbal
Naushad Ali
Khalid M. Alkharfy
Fahad I. Al-Jenoobi
Effects of Apigenin on Pharmacokinetics of Dasatinib and Probable Interaction Mechanism
Molecules
dasatinib
sinapic acid
herb drug interactions
PgP/MDR1
BCPR/ABCG2
CYP3A2
title Effects of Apigenin on Pharmacokinetics of Dasatinib and Probable Interaction Mechanism
title_full Effects of Apigenin on Pharmacokinetics of Dasatinib and Probable Interaction Mechanism
title_fullStr Effects of Apigenin on Pharmacokinetics of Dasatinib and Probable Interaction Mechanism
title_full_unstemmed Effects of Apigenin on Pharmacokinetics of Dasatinib and Probable Interaction Mechanism
title_short Effects of Apigenin on Pharmacokinetics of Dasatinib and Probable Interaction Mechanism
title_sort effects of apigenin on pharmacokinetics of dasatinib and probable interaction mechanism
topic dasatinib
sinapic acid
herb drug interactions
PgP/MDR1
BCPR/ABCG2
CYP3A2
url https://www.mdpi.com/1420-3049/28/4/1602
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