ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPR
The nuclear basket (NB) scaffold, a fibrillar structure anchored to the nuclear pore complex (NPC), is regarded as constructed of polypeptides of the coiled-coil dominated protein TPR to which other proteins can bind without contributing to the NB’s structural integrity. Here we report vertebrate pr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/10/8/1937 |
| _version_ | 1797524298535010304 |
|---|---|
| author | Philip Gunkel Haruki Iino Sandra Krull Volker C. Cordes |
| author_facet | Philip Gunkel Haruki Iino Sandra Krull Volker C. Cordes |
| author_sort | Philip Gunkel |
| collection | DOAJ |
| description | The nuclear basket (NB) scaffold, a fibrillar structure anchored to the nuclear pore complex (NPC), is regarded as constructed of polypeptides of the coiled-coil dominated protein TPR to which other proteins can bind without contributing to the NB’s structural integrity. Here we report vertebrate protein ZC3HC1 as a novel inherent constituent of the NB, common at the nuclear envelopes (NE) of proliferating and non-dividing, terminally differentiated cells of different morphogenetic origin. Formerly described as a protein of other functions, we instead present the NB component ZC3HC1 as a protein required for enabling distinct amounts of TPR to occur NB-appended, with such ZC3HC1-dependency applying to about half the total amount of TPR at the NEs of different somatic cell types. Furthermore, pointing to an NB structure more complex than previously anticipated, we discuss how ZC3HC1 and the ZC3HC1-dependent TPR polypeptides could enlarge the NB’s functional repertoire. |
| first_indexed | 2024-03-10T08:55:21Z |
| format | Article |
| id | doaj.art-34e5526b45684203bb137a53d7c704f7 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T08:55:21Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-34e5526b45684203bb137a53d7c704f72023-11-22T07:09:26ZengMDPI AGCells2073-44092021-07-01108193710.3390/cells10081937ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPRPhilip Gunkel0Haruki Iino1Sandra Krull2Volker C. Cordes3Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, GermanyMax Planck Institute for Biophysical Chemistry, D-37077 Göttingen, GermanyMax Planck Institute for Biophysical Chemistry, D-37077 Göttingen, GermanyMax Planck Institute for Biophysical Chemistry, D-37077 Göttingen, GermanyThe nuclear basket (NB) scaffold, a fibrillar structure anchored to the nuclear pore complex (NPC), is regarded as constructed of polypeptides of the coiled-coil dominated protein TPR to which other proteins can bind without contributing to the NB’s structural integrity. Here we report vertebrate protein ZC3HC1 as a novel inherent constituent of the NB, common at the nuclear envelopes (NE) of proliferating and non-dividing, terminally differentiated cells of different morphogenetic origin. Formerly described as a protein of other functions, we instead present the NB component ZC3HC1 as a protein required for enabling distinct amounts of TPR to occur NB-appended, with such ZC3HC1-dependency applying to about half the total amount of TPR at the NEs of different somatic cell types. Furthermore, pointing to an NB structure more complex than previously anticipated, we discuss how ZC3HC1 and the ZC3HC1-dependent TPR polypeptides could enlarge the NB’s functional repertoire.https://www.mdpi.com/2073-4409/10/8/1937NIPAnuclear basketnuclear interacting partner of ALKnuclear pore complexnucleoprotein TPRtranslocated promoter region |
| spellingShingle | Philip Gunkel Haruki Iino Sandra Krull Volker C. Cordes ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPR Cells NIPA nuclear basket nuclear interacting partner of ALK nuclear pore complex nucleoprotein TPR translocated promoter region |
| title | ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPR |
| title_full | ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPR |
| title_fullStr | ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPR |
| title_full_unstemmed | ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPR |
| title_short | ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPR |
| title_sort | zc3hc1 is a novel inherent component of the nuclear basket resident in a state of reciprocal dependence with tpr |
| topic | NIPA nuclear basket nuclear interacting partner of ALK nuclear pore complex nucleoprotein TPR translocated promoter region |
| url | https://www.mdpi.com/2073-4409/10/8/1937 |
| work_keys_str_mv | AT philipgunkel zc3hc1isanovelinherentcomponentofthenuclearbasketresidentinastateofreciprocaldependencewithtpr AT harukiiino zc3hc1isanovelinherentcomponentofthenuclearbasketresidentinastateofreciprocaldependencewithtpr AT sandrakrull zc3hc1isanovelinherentcomponentofthenuclearbasketresidentinastateofreciprocaldependencewithtpr AT volkerccordes zc3hc1isanovelinherentcomponentofthenuclearbasketresidentinastateofreciprocaldependencewithtpr |