Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J Mice
Xu Zhai,1 Meihua Gong,1,2 Yixuan Peng,1 Daping Yang1 1Department of Plastic Surgery, The 2nd Hospital of Harbin Medical University, Harbin, People’s Republic of China; 2Department of Plastic and Cosmetic Surgery, Shenzhen People’s Hospital, Second Affiliated Hospital of Jinan Uni...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-05-01
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| Series: | Clinical, Cosmetic and Investigational Dermatology |
| Subjects: | |
| Online Access: | https://www.dovepress.com/effects-of-uv-induced-photoaging-on-the-hair-follicle-cycle-of-c57bl6j-peer-reviewed-fulltext-article-CCID |
| _version_ | 1818572447373328384 |
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| author | Zhai X Gong M Peng Y Yang D |
| author_facet | Zhai X Gong M Peng Y Yang D |
| author_sort | Zhai X |
| collection | DOAJ |
| description | Xu Zhai,1 Meihua Gong,1,2 Yixuan Peng,1 Daping Yang1 1Department of Plastic Surgery, The 2nd Hospital of Harbin Medical University, Harbin, People’s Republic of China; 2Department of Plastic and Cosmetic Surgery, Shenzhen People’s Hospital, Second Affiliated Hospital of Jinan University Medical College, Shenzhen, People’s Republic of ChinaCorrespondence: Daping YangDepartment of Plastic Surgery, The 2nd Hospital of Harbin Medical University, Harbin, People’s Republic of ChinaTel/Fax +86-0451-86297062Email dapingyang1223@hotmail.comPurpose: To study the changes in the hair follicle cycle and related stem cells induced by photoaging to establish a mouse model of senescence in hair follicles.Methods: There were 54 C57BL6/J mice randomly divided into three groups. The UVA group and the UVB group underwent photoaging induced by UV lamps for 8 weeks. Changes in skin and the hair follicle cycle were compared by physical signs, dermoscopy, and hematoxylin and eosin and Masson’s staining in each group. Western blot, immunohistochemistry, and RT-qPCR were carried out to test canonical proteins and gene expression of the Wnt signaling pathway in the samples. Immunofluorescence was chosen to show variations in the stem cells related to the hair follicle cycle.Results: There were more gray hairs in the UVA group than the other groups (P< 0.05). Both diameter of the hair shaft and depth of hair root were significantly decreased in the UV groups (P< 0.05). Stem cells and melanocytes of the hair follicles were reduced in the UVA group. UV, especially UVB, up-regulated the expression of the Wnt signaling pathway and prolonged anagen and telogen phases in the hair follicles, compared with the control group (P< 0.05).Conclusion: By decreasing the number of stem cells related to hair follicles, UVA induces hair follicle photoaging characterized by hair follicle miniaturization and gray hairs. UV up-regulated the expression of the Wnt signaling pathway, and the hair follicle cycle was significantly prolonged by UVB.Keywords: UVA, photoaging, hair follicle cycle, HFSCs, Wnt |
| first_indexed | 2024-12-14T18:57:18Z |
| format | Article |
| id | doaj.art-34e8cfd76d144cb59983083c88bbd048 |
| institution | Directory Open Access Journal |
| issn | 1178-7015 |
| language | English |
| last_indexed | 2024-12-14T18:57:18Z |
| publishDate | 2021-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical, Cosmetic and Investigational Dermatology |
| spelling | doaj.art-34e8cfd76d144cb59983083c88bbd0482022-12-21T22:51:03ZengDove Medical PressClinical, Cosmetic and Investigational Dermatology1178-70152021-05-01Volume 1452753964867Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J MiceZhai XGong MPeng YYang DXu Zhai,1 Meihua Gong,1,2 Yixuan Peng,1 Daping Yang1 1Department of Plastic Surgery, The 2nd Hospital of Harbin Medical University, Harbin, People’s Republic of China; 2Department of Plastic and Cosmetic Surgery, Shenzhen People’s Hospital, Second Affiliated Hospital of Jinan University Medical College, Shenzhen, People’s Republic of ChinaCorrespondence: Daping YangDepartment of Plastic Surgery, The 2nd Hospital of Harbin Medical University, Harbin, People’s Republic of ChinaTel/Fax +86-0451-86297062Email dapingyang1223@hotmail.comPurpose: To study the changes in the hair follicle cycle and related stem cells induced by photoaging to establish a mouse model of senescence in hair follicles.Methods: There were 54 C57BL6/J mice randomly divided into three groups. The UVA group and the UVB group underwent photoaging induced by UV lamps for 8 weeks. Changes in skin and the hair follicle cycle were compared by physical signs, dermoscopy, and hematoxylin and eosin and Masson’s staining in each group. Western blot, immunohistochemistry, and RT-qPCR were carried out to test canonical proteins and gene expression of the Wnt signaling pathway in the samples. Immunofluorescence was chosen to show variations in the stem cells related to the hair follicle cycle.Results: There were more gray hairs in the UVA group than the other groups (P< 0.05). Both diameter of the hair shaft and depth of hair root were significantly decreased in the UV groups (P< 0.05). Stem cells and melanocytes of the hair follicles were reduced in the UVA group. UV, especially UVB, up-regulated the expression of the Wnt signaling pathway and prolonged anagen and telogen phases in the hair follicles, compared with the control group (P< 0.05).Conclusion: By decreasing the number of stem cells related to hair follicles, UVA induces hair follicle photoaging characterized by hair follicle miniaturization and gray hairs. UV up-regulated the expression of the Wnt signaling pathway, and the hair follicle cycle was significantly prolonged by UVB.Keywords: UVA, photoaging, hair follicle cycle, HFSCs, Wnthttps://www.dovepress.com/effects-of-uv-induced-photoaging-on-the-hair-follicle-cycle-of-c57bl6j-peer-reviewed-fulltext-article-CCIDuvaphotoaginghair follicle cyclehfscswnt |
| spellingShingle | Zhai X Gong M Peng Y Yang D Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J Mice Clinical, Cosmetic and Investigational Dermatology uva photoaging hair follicle cycle hfscs wnt |
| title | Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J Mice |
| title_full | Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J Mice |
| title_fullStr | Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J Mice |
| title_full_unstemmed | Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J Mice |
| title_short | Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J Mice |
| title_sort | effects of uv induced photoaging on the hair follicle cycle of c57bl6 j mice |
| topic | uva photoaging hair follicle cycle hfscs wnt |
| url | https://www.dovepress.com/effects-of-uv-induced-photoaging-on-the-hair-follicle-cycle-of-c57bl6j-peer-reviewed-fulltext-article-CCID |
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