An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum.
Photosynthetic diatoms are exposed to rapid and unpredictable changes in irradiance and spectral quality, and must be able to acclimate their light harvesting systems to varying light conditions. Molecular mechanisms behind light acclimation in diatoms are largely unknown. We set out to investigate...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-11-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2766053?pdf=render |
| _version_ | 1818315432583495680 |
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| author | Marianne Nymark Kristin C Valle Tore Brembu Kasper Hancke Per Winge Kjersti Andresen Geir Johnsen Atle M Bones |
| author_facet | Marianne Nymark Kristin C Valle Tore Brembu Kasper Hancke Per Winge Kjersti Andresen Geir Johnsen Atle M Bones |
| author_sort | Marianne Nymark |
| collection | DOAJ |
| description | Photosynthetic diatoms are exposed to rapid and unpredictable changes in irradiance and spectral quality, and must be able to acclimate their light harvesting systems to varying light conditions. Molecular mechanisms behind light acclimation in diatoms are largely unknown. We set out to investigate the mechanisms of high light acclimation in Phaeodactylum tricornutum using an integrated approach involving global transcriptional profiling, metabolite profiling and variable fluorescence technique. Algae cultures were acclimated to low light (LL), after which the cultures were transferred to high light (HL). Molecular, metabolic and physiological responses were studied at time points 0.5 h, 3 h, 6 h, 12 h, 24 h and 48 h after transfer to HL conditions. The integrated results indicate that the acclimation mechanisms in diatoms can be divided into an initial response phase (0-0.5 h), an intermediate acclimation phase (3-12 h) and a late acclimation phase (12-48 h). The initial phase is recognized by strong and rapid regulation of genes encoding proteins involved in photosynthesis, pigment metabolism and reactive oxygen species (ROS) scavenging systems. A significant increase in light protecting metabolites occur together with the induction of transcriptional processes involved in protection of cellular structures at this early phase. During the following phases, the metabolite profiling display a pronounced decrease in light harvesting pigments, whereas the variable fluorescence measurements show that the photosynthetic capacity increases strongly during the late acclimation phase. We show that P. tricornutum is capable of swift and efficient execution of photoprotective mechanisms, followed by changes in the composition of the photosynthetic machinery that enable the diatoms to utilize the excess energy available in HL. Central molecular players in light protection and acclimation to high irradiance have been identified. |
| first_indexed | 2024-12-13T09:05:27Z |
| format | Article |
| id | doaj.art-34ea71695a6844d889761b640791aa7e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T09:05:27Z |
| publishDate | 2009-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-34ea71695a6844d889761b640791aa7e2022-12-21T23:53:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-11-01411e774310.1371/journal.pone.0007743An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum.Marianne NymarkKristin C ValleTore BrembuKasper HanckePer WingeKjersti AndresenGeir JohnsenAtle M BonesPhotosynthetic diatoms are exposed to rapid and unpredictable changes in irradiance and spectral quality, and must be able to acclimate their light harvesting systems to varying light conditions. Molecular mechanisms behind light acclimation in diatoms are largely unknown. We set out to investigate the mechanisms of high light acclimation in Phaeodactylum tricornutum using an integrated approach involving global transcriptional profiling, metabolite profiling and variable fluorescence technique. Algae cultures were acclimated to low light (LL), after which the cultures were transferred to high light (HL). Molecular, metabolic and physiological responses were studied at time points 0.5 h, 3 h, 6 h, 12 h, 24 h and 48 h after transfer to HL conditions. The integrated results indicate that the acclimation mechanisms in diatoms can be divided into an initial response phase (0-0.5 h), an intermediate acclimation phase (3-12 h) and a late acclimation phase (12-48 h). The initial phase is recognized by strong and rapid regulation of genes encoding proteins involved in photosynthesis, pigment metabolism and reactive oxygen species (ROS) scavenging systems. A significant increase in light protecting metabolites occur together with the induction of transcriptional processes involved in protection of cellular structures at this early phase. During the following phases, the metabolite profiling display a pronounced decrease in light harvesting pigments, whereas the variable fluorescence measurements show that the photosynthetic capacity increases strongly during the late acclimation phase. We show that P. tricornutum is capable of swift and efficient execution of photoprotective mechanisms, followed by changes in the composition of the photosynthetic machinery that enable the diatoms to utilize the excess energy available in HL. Central molecular players in light protection and acclimation to high irradiance have been identified.http://europepmc.org/articles/PMC2766053?pdf=render |
| spellingShingle | Marianne Nymark Kristin C Valle Tore Brembu Kasper Hancke Per Winge Kjersti Andresen Geir Johnsen Atle M Bones An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum. PLoS ONE |
| title | An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum. |
| title_full | An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum. |
| title_fullStr | An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum. |
| title_full_unstemmed | An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum. |
| title_short | An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum. |
| title_sort | integrated analysis of molecular acclimation to high light in the marine diatom phaeodactylum tricornutum |
| url | http://europepmc.org/articles/PMC2766053?pdf=render |
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