Molecular characterization of Campylobacter spp. isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing
ABSTRACT: Chickens are the primary reservoirs of Campylobacter spp., mainly C. jejuni and C. coli, that cause human bacterial gastrointestinal infections. However, genomic characteristics and antimicrobial resistance of Campylobacter spp. in low- to middle-income countries need more comprehensive ex...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124000646 |
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| author | Doan Hoang Phu Tuempong Wongtawan Phitchayapak Wintachai Nguyen Thi Nhung Nguyen Thi Phuong Yen Juan Carrique-Mas Conny Turni Lida Omaleki Patrick J. Blackall Thotsapol Thomrongsuwannakij |
| author_facet | Doan Hoang Phu Tuempong Wongtawan Phitchayapak Wintachai Nguyen Thi Nhung Nguyen Thi Phuong Yen Juan Carrique-Mas Conny Turni Lida Omaleki Patrick J. Blackall Thotsapol Thomrongsuwannakij |
| author_sort | Doan Hoang Phu |
| collection | DOAJ |
| description | ABSTRACT: Chickens are the primary reservoirs of Campylobacter spp., mainly C. jejuni and C. coli, that cause human bacterial gastrointestinal infections. However, genomic characteristics and antimicrobial resistance of Campylobacter spp. in low- to middle-income countries need more comprehensive exploration. This study aimed to characterize 21 C. jejuni and 5 C. coli isolates from commercial broilers and native chickens using whole genome sequencing and compare them to 28 reference Campylobacter sequences. Among the 26 isolates, 13 sequence types (ST) were identified in C. jejuni and 5 ST in C. coli. The prominent ST was ST 2274 (5 isolates, 19.2%), followed by ST 51, 460, 2409, and 6455 (2 isolates in each ST, 7.7%), while all remaining ST (464, 536, 595, 2083, 6736, 6964, 8096, 10437, 828, 872, 900, 8237, and 13540) had 1 isolate per ST (3.8%). Six types of antimicrobial resistance genes (ant(6)-Ia, aph(3’)-III, blaOXA, cat, erm(B), and tet(O)) and one point mutations in the gyrA gene (Threonine-86-Isoleucine) and another in the rpsL gene (Lysine-43-Arginine) were detected. The blaOXA resistance gene was present in all isolates, the gyrA mutations was in 95.2% of C. jejuni and 80.0% of C. coli, and the tet(O) resistance gene in 76.2% of C. jejuni and 80.0% of C. coli. Additionally, 203 virulence-associated genes linked to 16 virulence factors were identified. In terms of phenotypic resistance, the C. jejuni isolates were all resistant to ciprofloxacin, enrofloxacin, and nalidixic acid, with lower levels of resistance to tetracycline (76.2%), tylosin (52.3%), erythromycin (23.8%), azithromycin (22.2%), and gentamicin (11.1%). Most C. coli isolates were resistant to all tested antimicrobials, while 1 C. coli was pan-susceptible except for tylosin. Single-nucleotide polymorphisms concordance varied widely, with differences of up to 13,375 single-nucleotide polymorphisms compared to the reference Campylobacter isolates, highlighting genetic divergence among comparative genomes. This study contributes to a deeper understanding of the molecular epidemiology of Campylobacter spp. in Thai chicken production systems. |
| first_indexed | 2024-03-08T03:36:00Z |
| format | Article |
| id | doaj.art-34ed2dfa20cd476c99c2beed5e2ca2ee |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T22:22:16Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-34ed2dfa20cd476c99c2beed5e2ca2ee2024-03-20T06:07:45ZengElsevierPoultry Science0032-57912024-04-011034103485Molecular characterization of Campylobacter spp. isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencingDoan Hoang Phu0Tuempong Wongtawan1Phitchayapak Wintachai2Nguyen Thi Nhung3Nguyen Thi Phuong Yen4Juan Carrique-Mas5Conny Turni6Lida Omaleki7Patrick J. Blackall8Thotsapol Thomrongsuwannakij9Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; Doctoral Program in Health Sciences, College of Graduate Studies, Walailak University, Nakhon Si Thammarat 80160, Thailand; Faculty of Animal Science and Veterinary Medicine, Nong Lam University, Ho Chi Minh City 70000, VietnamAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; Centre for One Health, Walailak University, Nakhon Si Thammarat 80160, ThailandSchool of Science, Walailak University, Nakhon Si Thammarat 80160, ThailandOxford University Clinical Research Unit, Ho Chi Minh City 70000, VietnamOxford University Clinical Research Unit, Ho Chi Minh City 70000, VietnamFood and Agriculture Organization of the United Nations, Ha Noi 10000, VietnamQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Queensland 4067, AustraliaQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Queensland 4067, AustraliaQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Queensland 4067, AustraliaAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; Centre for One Health, Walailak University, Nakhon Si Thammarat 80160, Thailand; Corresponding author:ABSTRACT: Chickens are the primary reservoirs of Campylobacter spp., mainly C. jejuni and C. coli, that cause human bacterial gastrointestinal infections. However, genomic characteristics and antimicrobial resistance of Campylobacter spp. in low- to middle-income countries need more comprehensive exploration. This study aimed to characterize 21 C. jejuni and 5 C. coli isolates from commercial broilers and native chickens using whole genome sequencing and compare them to 28 reference Campylobacter sequences. Among the 26 isolates, 13 sequence types (ST) were identified in C. jejuni and 5 ST in C. coli. The prominent ST was ST 2274 (5 isolates, 19.2%), followed by ST 51, 460, 2409, and 6455 (2 isolates in each ST, 7.7%), while all remaining ST (464, 536, 595, 2083, 6736, 6964, 8096, 10437, 828, 872, 900, 8237, and 13540) had 1 isolate per ST (3.8%). Six types of antimicrobial resistance genes (ant(6)-Ia, aph(3’)-III, blaOXA, cat, erm(B), and tet(O)) and one point mutations in the gyrA gene (Threonine-86-Isoleucine) and another in the rpsL gene (Lysine-43-Arginine) were detected. The blaOXA resistance gene was present in all isolates, the gyrA mutations was in 95.2% of C. jejuni and 80.0% of C. coli, and the tet(O) resistance gene in 76.2% of C. jejuni and 80.0% of C. coli. Additionally, 203 virulence-associated genes linked to 16 virulence factors were identified. In terms of phenotypic resistance, the C. jejuni isolates were all resistant to ciprofloxacin, enrofloxacin, and nalidixic acid, with lower levels of resistance to tetracycline (76.2%), tylosin (52.3%), erythromycin (23.8%), azithromycin (22.2%), and gentamicin (11.1%). Most C. coli isolates were resistant to all tested antimicrobials, while 1 C. coli was pan-susceptible except for tylosin. Single-nucleotide polymorphisms concordance varied widely, with differences of up to 13,375 single-nucleotide polymorphisms compared to the reference Campylobacter isolates, highlighting genetic divergence among comparative genomes. This study contributes to a deeper understanding of the molecular epidemiology of Campylobacter spp. in Thai chicken production systems.http://www.sciencedirect.com/science/article/pii/S0032579124000646Campylobacterwhole genome sequencingbioinformatic toolantimicrobial resistanceThailand |
| spellingShingle | Doan Hoang Phu Tuempong Wongtawan Phitchayapak Wintachai Nguyen Thi Nhung Nguyen Thi Phuong Yen Juan Carrique-Mas Conny Turni Lida Omaleki Patrick J. Blackall Thotsapol Thomrongsuwannakij Molecular characterization of Campylobacter spp. isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing Poultry Science Campylobacter whole genome sequencing bioinformatic tool antimicrobial resistance Thailand |
| title | Molecular characterization of Campylobacter spp. isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing |
| title_full | Molecular characterization of Campylobacter spp. isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing |
| title_fullStr | Molecular characterization of Campylobacter spp. isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing |
| title_full_unstemmed | Molecular characterization of Campylobacter spp. isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing |
| title_short | Molecular characterization of Campylobacter spp. isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing |
| title_sort | molecular characterization of campylobacter spp isolates obtained from commercial broilers and native chickens in southern thailand using whole genome sequencing |
| topic | Campylobacter whole genome sequencing bioinformatic tool antimicrobial resistance Thailand |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124000646 |
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