Expansion microscopy-based imaging of nuclear structures in cultured cells
Summary: Expansion microscopy is a sample preparation technique in which fixed and immunostained cells or tissues are embedded in a cross-linked network of swellable polyelectrolyte hydrogel that expands isotropically upon addition of deionized water. We utilize the X10 method for tenfold expansion...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-09-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721003373 |
| _version_ | 1818902965572861952 |
|---|---|
| author | Antoine Gaudreau-Lapierre Kirk Mulatz Jean-Claude Béïque Laura Trinkle-Mulcahy |
| author_facet | Antoine Gaudreau-Lapierre Kirk Mulatz Jean-Claude Béïque Laura Trinkle-Mulcahy |
| author_sort | Antoine Gaudreau-Lapierre |
| collection | DOAJ |
| description | Summary: Expansion microscopy is a sample preparation technique in which fixed and immunostained cells or tissues are embedded in a cross-linked network of swellable polyelectrolyte hydrogel that expands isotropically upon addition of deionized water. We utilize the X10 method for tenfold expansion of U2OS cells with concurrent DNA staining. A custom 3D-printed gel cutter and chambered slides minimize gel drift, facilitating analysis of the components of nuclear structures at nanoscale resolution by conventional microscopy or Airyscan confocal imaging.For complete information on the generation and use of this protocol, please refer to Do et al. (2020). |
| first_indexed | 2024-12-19T20:44:02Z |
| format | Article |
| id | doaj.art-34ee7600a962484dad3199d662412c36 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-19T20:44:02Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-34ee7600a962484dad3199d662412c362022-12-21T20:06:19ZengElsevierSTAR Protocols2666-16672021-09-0123100630Expansion microscopy-based imaging of nuclear structures in cultured cellsAntoine Gaudreau-Lapierre0Kirk Mulatz1Jean-Claude Béïque2Laura Trinkle-Mulcahy3Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Corresponding authorDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Brain and Mind Research Institute, University of Ottawa, Ottawa, ON K1H 8M5, CanadaDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Brain and Mind Research Institute, University of Ottawa, Ottawa, ON K1H 8M5, CanadaDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Corresponding authorSummary: Expansion microscopy is a sample preparation technique in which fixed and immunostained cells or tissues are embedded in a cross-linked network of swellable polyelectrolyte hydrogel that expands isotropically upon addition of deionized water. We utilize the X10 method for tenfold expansion of U2OS cells with concurrent DNA staining. A custom 3D-printed gel cutter and chambered slides minimize gel drift, facilitating analysis of the components of nuclear structures at nanoscale resolution by conventional microscopy or Airyscan confocal imaging.For complete information on the generation and use of this protocol, please refer to Do et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721003373Cell BiologyMicroscopy |
| spellingShingle | Antoine Gaudreau-Lapierre Kirk Mulatz Jean-Claude Béïque Laura Trinkle-Mulcahy Expansion microscopy-based imaging of nuclear structures in cultured cells STAR Protocols Cell Biology Microscopy |
| title | Expansion microscopy-based imaging of nuclear structures in cultured cells |
| title_full | Expansion microscopy-based imaging of nuclear structures in cultured cells |
| title_fullStr | Expansion microscopy-based imaging of nuclear structures in cultured cells |
| title_full_unstemmed | Expansion microscopy-based imaging of nuclear structures in cultured cells |
| title_short | Expansion microscopy-based imaging of nuclear structures in cultured cells |
| title_sort | expansion microscopy based imaging of nuclear structures in cultured cells |
| topic | Cell Biology Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721003373 |
| work_keys_str_mv | AT antoinegaudreaulapierre expansionmicroscopybasedimagingofnuclearstructuresinculturedcells AT kirkmulatz expansionmicroscopybasedimagingofnuclearstructuresinculturedcells AT jeanclaudebeique expansionmicroscopybasedimagingofnuclearstructuresinculturedcells AT lauratrinklemulcahy expansionmicroscopybasedimagingofnuclearstructuresinculturedcells |