Expansion microscopy-based imaging of nuclear structures in cultured cells
Summary: Expansion microscopy is a sample preparation technique in which fixed and immunostained cells or tissues are embedded in a cross-linked network of swellable polyelectrolyte hydrogel that expands isotropically upon addition of deionized water. We utilize the X10 method for tenfold expansion...
Main Authors: | Antoine Gaudreau-Lapierre, Kirk Mulatz, Jean-Claude Béïque, Laura Trinkle-Mulcahy |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-09-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721003373 |
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