Long intergenic non-coding RNAs (LincRNAs) identified by RNA-seq in breast cancer.
In an attempt to find the correlation of aberrant expression of long intergenic noncoding RNAs (lincRNAs) with cancer, twenty-five samples of breast cancer tissue and respective adjacent normal tissue were studied for the expression of lincRNAs by RNA-seq. Among the 538 lincRNAs studied, 124 lincRNA...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4118859?pdf=render |
| _version_ | 1828516477449273344 |
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| author | Xianfeng Ding Limin Zhu Ting Ji Xiping Zhang Fengmei Wang Shaoju Gan Ming Zhao Hongjian Yang |
| author_facet | Xianfeng Ding Limin Zhu Ting Ji Xiping Zhang Fengmei Wang Shaoju Gan Ming Zhao Hongjian Yang |
| author_sort | Xianfeng Ding |
| collection | DOAJ |
| description | In an attempt to find the correlation of aberrant expression of long intergenic noncoding RNAs (lincRNAs) with cancer, twenty-five samples of breast cancer tissue and respective adjacent normal tissue were studied for the expression of lincRNAs by RNA-seq. Among the 538 lincRNAs studied, 124 lincRNAs were exclusively expressed in cancer adjacent tissues and 62 lincRNAs were exclusively expressed in the cancer tissues. Furthermore, the expression of 134 lincRNAs was higher while 272 lower in breast cancer tissue compared with adjacent tissue. The expression of four selected lincRNAs (BC2, BC4, BC5, and BC8) was validated by semi-quantitative and real-time PCR. It was revealed that expression of lincRNA-BC5 was positively correlated with patients' age, pathological stage, and progesterone receptor concentration, while lincRNA-BC8 was negatively correlated with progesterone receptor expression. Higher expression of lincRNA-BC4 was seen in advanced breast cancer grade. LincRNA-BC2 showed no specific changes in the pathological features studied. Interactions between selected lincRNAs and breast cancer associated proteins were highly suggested by RPIseq based on the specific secondary structure. The results demonstrated that this group of lincRNAs was aberrantly expressed in breast cancer. They might play important roles in the function of oncogenes or tumor suppressors affecting the development and progression of breast cancer. |
| first_indexed | 2024-12-11T18:27:32Z |
| format | Article |
| id | doaj.art-34f13a98a0e94deda184c05ce0f92ad8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T18:27:32Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-34f13a98a0e94deda184c05ce0f92ad82022-12-22T00:55:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10327010.1371/journal.pone.0103270Long intergenic non-coding RNAs (LincRNAs) identified by RNA-seq in breast cancer.Xianfeng DingLimin ZhuTing JiXiping ZhangFengmei WangShaoju GanMing ZhaoHongjian YangIn an attempt to find the correlation of aberrant expression of long intergenic noncoding RNAs (lincRNAs) with cancer, twenty-five samples of breast cancer tissue and respective adjacent normal tissue were studied for the expression of lincRNAs by RNA-seq. Among the 538 lincRNAs studied, 124 lincRNAs were exclusively expressed in cancer adjacent tissues and 62 lincRNAs were exclusively expressed in the cancer tissues. Furthermore, the expression of 134 lincRNAs was higher while 272 lower in breast cancer tissue compared with adjacent tissue. The expression of four selected lincRNAs (BC2, BC4, BC5, and BC8) was validated by semi-quantitative and real-time PCR. It was revealed that expression of lincRNA-BC5 was positively correlated with patients' age, pathological stage, and progesterone receptor concentration, while lincRNA-BC8 was negatively correlated with progesterone receptor expression. Higher expression of lincRNA-BC4 was seen in advanced breast cancer grade. LincRNA-BC2 showed no specific changes in the pathological features studied. Interactions between selected lincRNAs and breast cancer associated proteins were highly suggested by RPIseq based on the specific secondary structure. The results demonstrated that this group of lincRNAs was aberrantly expressed in breast cancer. They might play important roles in the function of oncogenes or tumor suppressors affecting the development and progression of breast cancer.http://europepmc.org/articles/PMC4118859?pdf=render |
| spellingShingle | Xianfeng Ding Limin Zhu Ting Ji Xiping Zhang Fengmei Wang Shaoju Gan Ming Zhao Hongjian Yang Long intergenic non-coding RNAs (LincRNAs) identified by RNA-seq in breast cancer. PLoS ONE |
| title | Long intergenic non-coding RNAs (LincRNAs) identified by RNA-seq in breast cancer. |
| title_full | Long intergenic non-coding RNAs (LincRNAs) identified by RNA-seq in breast cancer. |
| title_fullStr | Long intergenic non-coding RNAs (LincRNAs) identified by RNA-seq in breast cancer. |
| title_full_unstemmed | Long intergenic non-coding RNAs (LincRNAs) identified by RNA-seq in breast cancer. |
| title_short | Long intergenic non-coding RNAs (LincRNAs) identified by RNA-seq in breast cancer. |
| title_sort | long intergenic non coding rnas lincrnas identified by rna seq in breast cancer |
| url | http://europepmc.org/articles/PMC4118859?pdf=render |
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