Fission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesis
Abstract Background The conserved NDR-family kinase Sid2p localizes to the contractile ring during fission yeast cytokinesis to promote ring constriction, septation, and completion of cell division. Previous studies have found that the Type 2 interphase node proteins Blt1p and Gef2p contribute to lo...
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Molecular and Cell Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12860-018-0182-z |
| _version_ | 1819043850424942592 |
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| author | Lois Kwon Emma M. Magee Alexis Crayton John W. Goss |
| author_facet | Lois Kwon Emma M. Magee Alexis Crayton John W. Goss |
| author_sort | Lois Kwon |
| collection | DOAJ |
| description | Abstract Background The conserved NDR-family kinase Sid2p localizes to the contractile ring during fission yeast cytokinesis to promote ring constriction, septation, and completion of cell division. Previous studies have found that the Type 2 interphase node proteins Blt1p and Gef2p contribute to localization of Sid2p and its regulatory protein Mob1p at the division site. However, their relative contributions and whether they operate in the same or parallel pathways has been unclear. In this study, we quantify the respective roles of Blt1p and Gef2p in Sid2p/Mob1p recruitment and characterize the effect of single and double deletion mutants on contractile ring dynamics and completion of cell division. Results Using quantitative confocal fluorescence microscopy, we measured Sid2p and Mob1p recruitment to the division site in blt1∆, gef2∆, and blt1∆/gef2∆ mutant cells. We observed an equivalent decrease in Sid2p/Mob1p localization for both single and double mutants. Though assembly of the contractile ring is normal in these mutants, the reduction in Sid2p/Mob1p at the division site delayed the onset of contractile ring constriction and completion of division. We quantified localization of Blt1p and Gef2p at the medial cortex throughout the cell cycle and found that Blt1p localization to interphase nodes and the contractile ring is independent of Gef2p. However, Gef2p localization to the contractile ring is decreased in blt1∆ mutants. Conclusions Blt1p and Gef2p work in the same pathway, rather than in parallel, to localize the NDR-family kinase Sid2p and its regulatory partner Mob1p to the division site, thereby promoting timely completion of cell division. Future studies are necessary to understand how additional fission yeast cytokinesis proteins work with these Type 2 interphase node components to promote Sid2p/Mob1p recruitment. |
| first_indexed | 2024-12-21T10:03:20Z |
| format | Article |
| id | doaj.art-34f74e2b6f9f40b7845ca7531f8ecb2d |
| institution | Directory Open Access Journal |
| issn | 2661-8850 |
| language | English |
| last_indexed | 2024-12-21T10:03:20Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Molecular and Cell Biology |
| spelling | doaj.art-34f74e2b6f9f40b7845ca7531f8ecb2d2022-12-21T19:07:53ZengBMCBMC Molecular and Cell Biology2661-88502019-01-0120111210.1186/s12860-018-0182-zFission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesisLois Kwon0Emma M. Magee1Alexis Crayton2John W. Goss3Department of Biological Sciences, Wellesley CollegeDepartment of Biological Sciences, Wellesley CollegeDepartment of Biological Sciences, Wellesley CollegeDepartment of Biological Sciences, Wellesley CollegeAbstract Background The conserved NDR-family kinase Sid2p localizes to the contractile ring during fission yeast cytokinesis to promote ring constriction, septation, and completion of cell division. Previous studies have found that the Type 2 interphase node proteins Blt1p and Gef2p contribute to localization of Sid2p and its regulatory protein Mob1p at the division site. However, their relative contributions and whether they operate in the same or parallel pathways has been unclear. In this study, we quantify the respective roles of Blt1p and Gef2p in Sid2p/Mob1p recruitment and characterize the effect of single and double deletion mutants on contractile ring dynamics and completion of cell division. Results Using quantitative confocal fluorescence microscopy, we measured Sid2p and Mob1p recruitment to the division site in blt1∆, gef2∆, and blt1∆/gef2∆ mutant cells. We observed an equivalent decrease in Sid2p/Mob1p localization for both single and double mutants. Though assembly of the contractile ring is normal in these mutants, the reduction in Sid2p/Mob1p at the division site delayed the onset of contractile ring constriction and completion of division. We quantified localization of Blt1p and Gef2p at the medial cortex throughout the cell cycle and found that Blt1p localization to interphase nodes and the contractile ring is independent of Gef2p. However, Gef2p localization to the contractile ring is decreased in blt1∆ mutants. Conclusions Blt1p and Gef2p work in the same pathway, rather than in parallel, to localize the NDR-family kinase Sid2p and its regulatory partner Mob1p to the division site, thereby promoting timely completion of cell division. Future studies are necessary to understand how additional fission yeast cytokinesis proteins work with these Type 2 interphase node components to promote Sid2p/Mob1p recruitment.http://link.springer.com/article/10.1186/s12860-018-0182-zSeptation initiation networkCytokinesisFission yeastBlt1pGef2pSid2p |
| spellingShingle | Lois Kwon Emma M. Magee Alexis Crayton John W. Goss Fission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesis BMC Molecular and Cell Biology Septation initiation network Cytokinesis Fission yeast Blt1p Gef2p Sid2p |
| title | Fission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesis |
| title_full | Fission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesis |
| title_fullStr | Fission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesis |
| title_full_unstemmed | Fission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesis |
| title_short | Fission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesis |
| title_sort | fission yeast type 2 node proteins blt1p and gef2p cooperate to ensure timely completion of cytokinesis |
| topic | Septation initiation network Cytokinesis Fission yeast Blt1p Gef2p Sid2p |
| url | http://link.springer.com/article/10.1186/s12860-018-0182-z |
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