Effect of Hecogenin on DNA instability
Hecogenin is a sapogenin found in Agave species in high quantities and is responsible for the many therapeutic effects of these medicinal plants. In addition, this compound is also widely used in the pharmaceutical industry as a precursor for the synthesis of steroidal hormones and anti-inflammatory...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-01-01
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| Series: | Toxicology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750016300464 |
| _version_ | 1811230541801848832 |
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| author | Marina Sampaio Cruz Sarah Cabral Barroso Julio Alejandro Navoni Maria Madalena Rocha Silva Teles José Maria Barbosa-Filho Hugo Alexandre de Oliveira Rocha Viviane Souza do Amaral |
| author_facet | Marina Sampaio Cruz Sarah Cabral Barroso Julio Alejandro Navoni Maria Madalena Rocha Silva Teles José Maria Barbosa-Filho Hugo Alexandre de Oliveira Rocha Viviane Souza do Amaral |
| author_sort | Marina Sampaio Cruz |
| collection | DOAJ |
| description | Hecogenin is a sapogenin found in Agave species in high quantities and is responsible for the many therapeutic effects of these medicinal plants. In addition, this compound is also widely used in the pharmaceutical industry as a precursor for the synthesis of steroidal hormones and anti-inflammatory drugs. Despite Hecogenin being widely used, little is known about its toxicological properties. Therefore, the present study aimed to investigate the cytotoxic, genotoxic and mutagenic effects of Hecogenin on HepG2 cells. Cytotoxicity was analyzed using the MTT test. Then, genotoxic and mutagenic potentials were assessed by comet assay and cytokinesis-block micronucleus assay, respectively. Cytotoxic effect was observed only when cells were exposed to concentrations of Hecogenin equal or higher than 100 μM. Although a lower concentration of Hecogenin caused DNA damage, a reduction on nuclear mutagenic markers in HepG2 cells was observed. The results indicated that Hecogenin treatment generated DNA damage, but in fact it would be repaired, avoiding dissemination of the damage throughout the cell division. Further studies need to be performed to confirm the observed protective effect of Hecogenin against genomic instability. Keywords: Hecogenin, CBMN, Genotoxicity, Comet assay |
| first_indexed | 2024-04-12T10:31:21Z |
| format | Article |
| id | doaj.art-34f84a38f1d44584a1e43b300d80eb88 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-04-12T10:31:21Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-34f84a38f1d44584a1e43b300d80eb882022-12-22T03:36:51ZengElsevierToxicology Reports2214-75002016-01-013539543Effect of Hecogenin on DNA instabilityMarina Sampaio Cruz0Sarah Cabral Barroso1Julio Alejandro Navoni2Maria Madalena Rocha Silva Teles3José Maria Barbosa-Filho4Hugo Alexandre de Oliveira Rocha5Viviane Souza do Amaral6Programa de Pós-Graduação em Bioquímica, Universidade Federal do Rio Grande do Norte, Av. Sen. Salgado Filho 3000, 59078-970 Natal, RN, BrazilLaboratório de Mutagênese Ambiental, Universidade Federal do Rio Grande do Norte, Av. Sen. Salgado Filho 3000, 59078-970 Natal, RN, BrazilPrograma de Pós-Graduação em Desenvolvimento e Meio Ambiente, Universidade Federal do Rio Grande do Norte, Av. Sen. Salgado Filho 3000, 59078-970 Natal, RN, BrazilLaboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Cidade Universitária, 58051900 João Pessoa, PB, BrazilLaboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Cidade Universitária, 58051900 João Pessoa, PB, BrazilPrograma de Pós-Graduação em Bioquímica, Universidade Federal do Rio Grande do Norte, Av. Sen. Salgado Filho 3000, 59078-970 Natal, RN, BrazilPrograma de Pós-Graduação em Bioquímica, Universidade Federal do Rio Grande do Norte, Av. Sen. Salgado Filho 3000, 59078-970 Natal, RN, Brazil; Laboratório de Mutagênese Ambiental, Universidade Federal do Rio Grande do Norte, Av. Sen. Salgado Filho 3000, 59078-970 Natal, RN, Brazil; Programa de Pós-Graduação em Desenvolvimento e Meio Ambiente, Universidade Federal do Rio Grande do Norte, Av. Sen. Salgado Filho 3000, 59078-970 Natal, RN, Brazil; Corresponding author at: Departamento de Biologia Celular e Genética, Universidade Federal do Rio Grande do Norte, Av. Sen. Salgado Filho 3000–Lagoa Nova, CEP 59078-970, Natal, RN/Brazil.Hecogenin is a sapogenin found in Agave species in high quantities and is responsible for the many therapeutic effects of these medicinal plants. In addition, this compound is also widely used in the pharmaceutical industry as a precursor for the synthesis of steroidal hormones and anti-inflammatory drugs. Despite Hecogenin being widely used, little is known about its toxicological properties. Therefore, the present study aimed to investigate the cytotoxic, genotoxic and mutagenic effects of Hecogenin on HepG2 cells. Cytotoxicity was analyzed using the MTT test. Then, genotoxic and mutagenic potentials were assessed by comet assay and cytokinesis-block micronucleus assay, respectively. Cytotoxic effect was observed only when cells were exposed to concentrations of Hecogenin equal or higher than 100 μM. Although a lower concentration of Hecogenin caused DNA damage, a reduction on nuclear mutagenic markers in HepG2 cells was observed. The results indicated that Hecogenin treatment generated DNA damage, but in fact it would be repaired, avoiding dissemination of the damage throughout the cell division. Further studies need to be performed to confirm the observed protective effect of Hecogenin against genomic instability. Keywords: Hecogenin, CBMN, Genotoxicity, Comet assayhttp://www.sciencedirect.com/science/article/pii/S2214750016300464 |
| spellingShingle | Marina Sampaio Cruz Sarah Cabral Barroso Julio Alejandro Navoni Maria Madalena Rocha Silva Teles José Maria Barbosa-Filho Hugo Alexandre de Oliveira Rocha Viviane Souza do Amaral Effect of Hecogenin on DNA instability Toxicology Reports |
| title | Effect of Hecogenin on DNA instability |
| title_full | Effect of Hecogenin on DNA instability |
| title_fullStr | Effect of Hecogenin on DNA instability |
| title_full_unstemmed | Effect of Hecogenin on DNA instability |
| title_short | Effect of Hecogenin on DNA instability |
| title_sort | effect of hecogenin on dna instability |
| url | http://www.sciencedirect.com/science/article/pii/S2214750016300464 |
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