Molecular determinants that govern scaRNA processing by Drosha/DGCR8
The Cajal body (CB) is a subnuclear domain that participates in the biogenesis of many different types of ribonucleoproteins (RNPs), including small nuclear RNPs (snRNPs), small Cajal body-specific RNPs (scaRNPs) and telomerase. Most scaRNAs, the RNA component of scaRNPs, accumulate in CBs. However,...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2020-10-01
|
| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/9/10/bio054619 |
| _version_ | 1818900997813043200 |
|---|---|
| author | Douglas M. McLaurin Madelyn K. Logan Katheryn E. Lett Michael D. Hebert |
| author_facet | Douglas M. McLaurin Madelyn K. Logan Katheryn E. Lett Michael D. Hebert |
| author_sort | Douglas M. McLaurin |
| collection | DOAJ |
| description | The Cajal body (CB) is a subnuclear domain that participates in the biogenesis of many different types of ribonucleoproteins (RNPs), including small nuclear RNPs (snRNPs), small Cajal body-specific RNPs (scaRNPs) and telomerase. Most scaRNAs, the RNA component of scaRNPs, accumulate in CBs. However, there are three scaRNAs (scaRNA 2, 9, and 17) that are known to be processed into small, nucleolar-enriched fragments. Evidence suggests that these fragments are packaged into a new class of RNPs, called regulatory RNPs (regRNPs), and may modify small nucleolar RNP (snoRNP) activity, thus playing a role in rRNA modification. However, the mechanism by which these fragments are produced is unknown. Previous work has reported the involvement of Drosha and DGCR8 in the cleavage of primary-scaRNA9. Here, we expand on that knowledge by identifying sequence elements necessary for the efficient production of these RNA fragments and demonstrate that primary scaRNA 2 and 17 are also processed by the Drosha-DGCR8 complex. Collectively, our work establishes new factors in the scaRNP biogenesis pathway and adds to the ever-expanding list of noncanonical functions for the microprocessor complex. |
| first_indexed | 2024-12-19T20:12:45Z |
| format | Article |
| id | doaj.art-34fcc0ef68024542aa349036fb86ae50 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-19T20:12:45Z |
| publishDate | 2020-10-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-34fcc0ef68024542aa349036fb86ae502022-12-21T20:07:16ZengThe Company of BiologistsBiology Open2046-63902020-10-0191010.1242/bio.054619054619Molecular determinants that govern scaRNA processing by Drosha/DGCR8Douglas M. McLaurin0Madelyn K. Logan1Katheryn E. Lett2Michael D. Hebert3 Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA The Cajal body (CB) is a subnuclear domain that participates in the biogenesis of many different types of ribonucleoproteins (RNPs), including small nuclear RNPs (snRNPs), small Cajal body-specific RNPs (scaRNPs) and telomerase. Most scaRNAs, the RNA component of scaRNPs, accumulate in CBs. However, there are three scaRNAs (scaRNA 2, 9, and 17) that are known to be processed into small, nucleolar-enriched fragments. Evidence suggests that these fragments are packaged into a new class of RNPs, called regulatory RNPs (regRNPs), and may modify small nucleolar RNP (snoRNP) activity, thus playing a role in rRNA modification. However, the mechanism by which these fragments are produced is unknown. Previous work has reported the involvement of Drosha and DGCR8 in the cleavage of primary-scaRNA9. Here, we expand on that knowledge by identifying sequence elements necessary for the efficient production of these RNA fragments and demonstrate that primary scaRNA 2 and 17 are also processed by the Drosha-DGCR8 complex. Collectively, our work establishes new factors in the scaRNP biogenesis pathway and adds to the ever-expanding list of noncanonical functions for the microprocessor complex.http://bio.biologists.org/content/9/10/bio054619cajal body (cb)small cajal body-specific rna (scarna)microrna (mirna) |
| spellingShingle | Douglas M. McLaurin Madelyn K. Logan Katheryn E. Lett Michael D. Hebert Molecular determinants that govern scaRNA processing by Drosha/DGCR8 Biology Open cajal body (cb) small cajal body-specific rna (scarna) microrna (mirna) |
| title | Molecular determinants that govern scaRNA processing by Drosha/DGCR8 |
| title_full | Molecular determinants that govern scaRNA processing by Drosha/DGCR8 |
| title_fullStr | Molecular determinants that govern scaRNA processing by Drosha/DGCR8 |
| title_full_unstemmed | Molecular determinants that govern scaRNA processing by Drosha/DGCR8 |
| title_short | Molecular determinants that govern scaRNA processing by Drosha/DGCR8 |
| title_sort | molecular determinants that govern scarna processing by drosha dgcr8 |
| topic | cajal body (cb) small cajal body-specific rna (scarna) microrna (mirna) |
| url | http://bio.biologists.org/content/9/10/bio054619 |
| work_keys_str_mv | AT douglasmmclaurin moleculardeterminantsthatgovernscarnaprocessingbydroshadgcr8 AT madelynklogan moleculardeterminantsthatgovernscarnaprocessingbydroshadgcr8 AT katherynelett moleculardeterminantsthatgovernscarnaprocessingbydroshadgcr8 AT michaeldhebert moleculardeterminantsthatgovernscarnaprocessingbydroshadgcr8 |