Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes
Although advances in protein assembly preparation have provided a new platform for drug delivery during tissue engineering, achieving long-term controlled exosome delivery remains a significant challenge. Diffusion-dominated exosome release using protein hydrogels results in burst release of exosome...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-02-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21003030 |
| _version_ | 1797203352767954944 |
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| author | Muyang Sun Qi Li Huilei Yu Jin Cheng Nier Wu Weili Shi Fengyuan Zhao Zhenxing Shao Qingyang Meng Haifeng Chen Xiaoqing Hu Yingfang Ao |
| author_facet | Muyang Sun Qi Li Huilei Yu Jin Cheng Nier Wu Weili Shi Fengyuan Zhao Zhenxing Shao Qingyang Meng Haifeng Chen Xiaoqing Hu Yingfang Ao |
| author_sort | Muyang Sun |
| collection | DOAJ |
| description | Although advances in protein assembly preparation have provided a new platform for drug delivery during tissue engineering, achieving long-term controlled exosome delivery remains a significant challenge. Diffusion-dominated exosome release using protein hydrogels results in burst release of exosomes. Here, a fibroin-based cryo-sponge was developed to provide controlled exosome release. Fibroin chains can self-assemble into silk I structures under ice-cold conditions when annealed above the glass transition temperature. Exosome release is enzyme-responsive, with rates primarily determined by enzymatic degradation of the scaffolds. In vivo experiments have demonstrated that exosomes remain in undigested sponge material for two months, superior to their retention in fibrin glue, a commonly used biomaterial in clinical practice. Fibroin cryo-sponges were implanted subcutaneously in nude mice. The exosome-containing sponge group exhibited better neovascularization and tissue ingrowth effects, demonstrating the efficacy of this exosome-encapsulating strategy by realizing sustained release and maintaining exosome bioactivity. These silk fibroin cryo-sponges containing exosomes provide a new platform for future studies of exosome therapy. |
| first_indexed | 2024-04-24T08:17:58Z |
| format | Article |
| id | doaj.art-3502177ea2cd43e4b9658f3248dcfa45 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:17:58Z |
| publishDate | 2022-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-3502177ea2cd43e4b9658f3248dcfa452024-04-17T02:48:24ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2022-02-018505514Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomesMuyang Sun0Qi Li1Huilei Yu2Jin Cheng3Nier Wu4Weili Shi5Fengyuan Zhao6Zhenxing Shao7Qingyang Meng8Haifeng Chen9Xiaoqing Hu10Yingfang Ao11Department of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, China; Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, 100871, ChinaDepartment of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, ChinaDepartment of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, ChinaDepartment of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, ChinaDepartment of Biomedical Engineering, College of Future Technology, Peking University, Beijing, 100871, ChinaDepartment of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, ChinaDepartment of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, ChinaDepartment of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, ChinaDepartment of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, ChinaDepartment of Biomedical Engineering, College of Future Technology, Peking University, Beijing, 100871, China; Corresponding author.Department of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, China; Corresponding author.Department of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, China; Corresponding author.Although advances in protein assembly preparation have provided a new platform for drug delivery during tissue engineering, achieving long-term controlled exosome delivery remains a significant challenge. Diffusion-dominated exosome release using protein hydrogels results in burst release of exosomes. Here, a fibroin-based cryo-sponge was developed to provide controlled exosome release. Fibroin chains can self-assemble into silk I structures under ice-cold conditions when annealed above the glass transition temperature. Exosome release is enzyme-responsive, with rates primarily determined by enzymatic degradation of the scaffolds. In vivo experiments have demonstrated that exosomes remain in undigested sponge material for two months, superior to their retention in fibrin glue, a commonly used biomaterial in clinical practice. Fibroin cryo-sponges were implanted subcutaneously in nude mice. The exosome-containing sponge group exhibited better neovascularization and tissue ingrowth effects, demonstrating the efficacy of this exosome-encapsulating strategy by realizing sustained release and maintaining exosome bioactivity. These silk fibroin cryo-sponges containing exosomes provide a new platform for future studies of exosome therapy.http://www.sciencedirect.com/science/article/pii/S2452199X21003030ExosomesSilk fibroinCryo-spongeSustained drug deliveryEnzyme-responsive |
| spellingShingle | Muyang Sun Qi Li Huilei Yu Jin Cheng Nier Wu Weili Shi Fengyuan Zhao Zhenxing Shao Qingyang Meng Haifeng Chen Xiaoqing Hu Yingfang Ao Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes Bioactive Materials Exosomes Silk fibroin Cryo-sponge Sustained drug delivery Enzyme-responsive |
| title | Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes |
| title_full | Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes |
| title_fullStr | Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes |
| title_full_unstemmed | Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes |
| title_short | Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes |
| title_sort | cryo self assembled silk fibroin sponge as a biodegradable platform for enzyme responsive delivery of exosomes |
| topic | Exosomes Silk fibroin Cryo-sponge Sustained drug delivery Enzyme-responsive |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X21003030 |
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