Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos
Antimicrobial resistant (AMR) Enterobacterales are widely distributed among the healthy population of the Indochinese peninsula, including Laos. However, the local reservoir of these pathogens are currently not known and possible sources such as agricultural settings and food have rarely been analyz...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.708182/full |
| _version_ | 1830481111907565568 |
|---|---|
| author | Aline I. Moser Esther Kuenzli Esther Kuenzli Esther Kuenzli Edgar I. Campos-Madueno Thomas Büdel Sayaphet Rattanavong Manivanh Vongsouvath Christoph Hatz Christoph Hatz Christoph Hatz Christoph Hatz Andrea Endimiani |
| author_facet | Aline I. Moser Esther Kuenzli Esther Kuenzli Esther Kuenzli Edgar I. Campos-Madueno Thomas Büdel Sayaphet Rattanavong Manivanh Vongsouvath Christoph Hatz Christoph Hatz Christoph Hatz Christoph Hatz Andrea Endimiani |
| author_sort | Aline I. Moser |
| collection | DOAJ |
| description | Antimicrobial resistant (AMR) Enterobacterales are widely distributed among the healthy population of the Indochinese peninsula, including Laos. However, the local reservoir of these pathogens are currently not known and possible sources such as agricultural settings and food have rarely been analyzed. In this work, we investigated the extended-spectrum cephalosporin- (ESC-) and colistin-resistant Escherichia coli strains (CST-R-Ec) isolated from the gut of local people, feces of poultry, and from chicken meat (60 samples each group) in Laos. Whole-genome sequencing (WGS) analysis based on both short- and long-read sequencing approaches were implemented. The following prevalence of ESC-R-Ec and CST-R-Ec were recorded, respectively: local people (70 and 15%), poultry (20 and 23.3%), and chicken meat (21.7 and 13.3%). Core-genome analysis, coupled with sequence type (ST)/core-genome ST (cgST) definitions, indicated that no common AMR-Ec clones were spreading among the different settings. ESC-R-Ec mostly possessed blaCTX–M–15 and blaCTX–M–55 associated to ISEcp1 or IS26. The majority of CST-R-Ec carried mcr-1 on IncX4, IncI2, IncP1, and IncHI1 plasmids similar or identical to those described worldwide; strains with chromosomal mcr-1 or possessing plasmid-mediated mcr-3 were also found. These results indicate a high prevalence of AMR-Ec in the local population, poultry, and chicken meat. While we did not observe the same clones among the three settings, most of the blaCTX–Ms and mcr-1/-3 were associated with mobile-genetic elements, indicating that horizontal gene transfer may play an important role in the dissemination of AMR-Ec in Laos. More studies should be planned to better understand the extent and dynamics of this phenomenon. |
| first_indexed | 2024-12-21T17:13:15Z |
| format | Article |
| id | doaj.art-35093056c1cd45be8e13e490e653314f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T17:13:15Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-35093056c1cd45be8e13e490e653314f2022-12-21T18:56:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-07-011210.3389/fmicb.2021.708182708182Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in LaosAline I. Moser0Esther Kuenzli1Esther Kuenzli2Esther Kuenzli3Edgar I. Campos-Madueno4Thomas Büdel5Sayaphet Rattanavong6Manivanh Vongsouvath7Christoph Hatz8Christoph Hatz9Christoph Hatz10Christoph Hatz11Andrea Endimiani12Institute for Infectious Diseases, University of Bern, Bern, SwitzerlandDepartment of Public Health, Epidemiology, Biostatistics and Prevention Institute, University of Zurich, Zurich, SwitzerlandSwiss Tropical and Public Health Institute, Basel, SwitzerlandUniversity of Basel, Basel, SwitzerlandInstitute for Infectious Diseases, University of Bern, Bern, SwitzerlandInstitute for Infectious Diseases, University of Bern, Bern, SwitzerlandMicrobiology Laboratory, Mahosot Hospital, Vientiane, LaosMicrobiology Laboratory, Mahosot Hospital, Vientiane, LaosDepartment of Public Health, Epidemiology, Biostatistics and Prevention Institute, University of Zurich, Zurich, SwitzerlandSwiss Tropical and Public Health Institute, Basel, SwitzerlandUniversity of Basel, Basel, SwitzerlandDivision of Infectious Diseases and Hospital Epidemiology, Kantonsspital St. Gallen, St. Gallen, SwitzerlandInstitute for Infectious Diseases, University of Bern, Bern, SwitzerlandAntimicrobial resistant (AMR) Enterobacterales are widely distributed among the healthy population of the Indochinese peninsula, including Laos. However, the local reservoir of these pathogens are currently not known and possible sources such as agricultural settings and food have rarely been analyzed. In this work, we investigated the extended-spectrum cephalosporin- (ESC-) and colistin-resistant Escherichia coli strains (CST-R-Ec) isolated from the gut of local people, feces of poultry, and from chicken meat (60 samples each group) in Laos. Whole-genome sequencing (WGS) analysis based on both short- and long-read sequencing approaches were implemented. The following prevalence of ESC-R-Ec and CST-R-Ec were recorded, respectively: local people (70 and 15%), poultry (20 and 23.3%), and chicken meat (21.7 and 13.3%). Core-genome analysis, coupled with sequence type (ST)/core-genome ST (cgST) definitions, indicated that no common AMR-Ec clones were spreading among the different settings. ESC-R-Ec mostly possessed blaCTX–M–15 and blaCTX–M–55 associated to ISEcp1 or IS26. The majority of CST-R-Ec carried mcr-1 on IncX4, IncI2, IncP1, and IncHI1 plasmids similar or identical to those described worldwide; strains with chromosomal mcr-1 or possessing plasmid-mediated mcr-3 were also found. These results indicate a high prevalence of AMR-Ec in the local population, poultry, and chicken meat. While we did not observe the same clones among the three settings, most of the blaCTX–Ms and mcr-1/-3 were associated with mobile-genetic elements, indicating that horizontal gene transfer may play an important role in the dissemination of AMR-Ec in Laos. More studies should be planned to better understand the extent and dynamics of this phenomenon.https://www.frontiersin.org/articles/10.3389/fmicb.2021.708182/fullmcr-1mcr-3CTX-ME. coliLaosgut colonization |
| spellingShingle | Aline I. Moser Esther Kuenzli Esther Kuenzli Esther Kuenzli Edgar I. Campos-Madueno Thomas Büdel Sayaphet Rattanavong Manivanh Vongsouvath Christoph Hatz Christoph Hatz Christoph Hatz Christoph Hatz Andrea Endimiani Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos Frontiers in Microbiology mcr-1 mcr-3 CTX-M E. coli Laos gut colonization |
| title | Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos |
| title_full | Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos |
| title_fullStr | Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos |
| title_full_unstemmed | Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos |
| title_short | Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos |
| title_sort | antimicrobial resistant escherichia coli strains and their plasmids in people poultry and chicken meat in laos |
| topic | mcr-1 mcr-3 CTX-M E. coli Laos gut colonization |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.708182/full |
| work_keys_str_mv | AT alineimoser antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT estherkuenzli antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT estherkuenzli antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT estherkuenzli antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT edgaricamposmadueno antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT thomasbudel antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT sayaphetrattanavong antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT manivanhvongsouvath antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT christophhatz antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT christophhatz antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT christophhatz antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT christophhatz antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos AT andreaendimiani antimicrobialresistantescherichiacolistrainsandtheirplasmidsinpeoplepoultryandchickenmeatinlaos |