Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method
Cryopreservation by droplet vitrification was applied to hazelnut (<i>Corylus avellana</i> L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i>in vitro</i> growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/11/494 |
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| author | Alessandra Sgueglia Andrea Frattarelli Adele Gentile Gaia Urbinati Simona Lucioli Maria Antonietta Germanà Emilia Caboni |
| author_facet | Alessandra Sgueglia Andrea Frattarelli Adele Gentile Gaia Urbinati Simona Lucioli Maria Antonietta Germanà Emilia Caboni |
| author_sort | Alessandra Sgueglia |
| collection | DOAJ |
| description | Cryopreservation by droplet vitrification was applied to hazelnut (<i>Corylus avellana</i> L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i>in vitro</i> growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min, then transferred to a droplet of vitrification solution, placed on a strip of aluminium foil, and plunged into liquid nitrogen (LN). Additionally, the effect on the recovery of the mother plant after cryopreservation was evaluated, following a cold pre-treatment at 4 °C for 3 months. The highest regrowth percentage (56.7%) was obtained after applying PVS3 for 60 min, while the application of PVS2 for the same amount of time reduced regrowth to 41.5%. Increasing the exposure to vitrification solutions to 90 min reduced regrowth to 43.3% when PVS3 was applied, and 35.6% if PVS2 was used. The cold pre-treatment on the mother plant did not significantly improve overall regrowth. The cryopreservation process did not decline the rooting ability of the recovered shoots. |
| first_indexed | 2024-03-10T05:26:24Z |
| format | Article |
| id | doaj.art-350b3eb3b04248bba32a900662003198 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T05:26:24Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-350b3eb3b04248bba32a9006620031982023-11-22T23:34:30ZengMDPI AGHorticulturae2311-75242021-11-0171149410.3390/horticulturae7110494Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification MethodAlessandra Sgueglia0Andrea Frattarelli1Adele Gentile2Gaia Urbinati3Simona Lucioli4Maria Antonietta Germanà5Emilia Caboni6Research Centre for Olive Citrus and Fruit Tree (CREA-OFA), Council for Agricultural Research and Economics, 00134 Rome, ItalyResearch Centre for Olive Citrus and Fruit Tree (CREA-OFA), Council for Agricultural Research and Economics, 00134 Rome, ItalyResearch Centre for Olive Citrus and Fruit Tree (CREA-OFA), Council for Agricultural Research and Economics, 00134 Rome, ItalyResearch Centre for Olive Citrus and Fruit Tree (CREA-OFA), Council for Agricultural Research and Economics, 00134 Rome, ItalyResearch Centre for Olive Citrus and Fruit Tree (CREA-OFA), Council for Agricultural Research and Economics, 00134 Rome, ItalyDepartment of Agricultural Food and Forest Sciences (SAAF), University of Palermo, 90133 Palermo, ItalyResearch Centre for Olive Citrus and Fruit Tree (CREA-OFA), Council for Agricultural Research and Economics, 00134 Rome, ItalyCryopreservation by droplet vitrification was applied to hazelnut (<i>Corylus avellana</i> L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i>in vitro</i> growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min, then transferred to a droplet of vitrification solution, placed on a strip of aluminium foil, and plunged into liquid nitrogen (LN). Additionally, the effect on the recovery of the mother plant after cryopreservation was evaluated, following a cold pre-treatment at 4 °C for 3 months. The highest regrowth percentage (56.7%) was obtained after applying PVS3 for 60 min, while the application of PVS2 for the same amount of time reduced regrowth to 41.5%. Increasing the exposure to vitrification solutions to 90 min reduced regrowth to 43.3% when PVS3 was applied, and 35.6% if PVS2 was used. The cold pre-treatment on the mother plant did not significantly improve overall regrowth. The cryopreservation process did not decline the rooting ability of the recovered shoots.https://www.mdpi.com/2311-7524/7/11/494<i>in vitro</i> culturelength of dehydrationPVS2PVS3regrowthrooting |
| spellingShingle | Alessandra Sgueglia Andrea Frattarelli Adele Gentile Gaia Urbinati Simona Lucioli Maria Antonietta Germanà Emilia Caboni Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method Horticulturae <i>in vitro</i> culture length of dehydration PVS2 PVS3 regrowth rooting |
| title | Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method |
| title_full | Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method |
| title_fullStr | Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method |
| title_full_unstemmed | Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method |
| title_short | Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method |
| title_sort | cryopreservation of hazelnut i corylus avellana i l axillary buds from i in vitro i shoots using the droplet vitrification method |
| topic | <i>in vitro</i> culture length of dehydration PVS2 PVS3 regrowth rooting |
| url | https://www.mdpi.com/2311-7524/7/11/494 |
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