Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1
We investigated whether 14 phenolic compounds isolated from Artemisia argyi could prevent the apoptotic damage caused by iodixanol, an iodinated contrast agent, on LLC-PK1 cells. Iodixanol was used to induce cytotoxicity in LLC-PK1 cells. Apoptotic cell death was observed as the fluorescence intensi...
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| Format: | Article |
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MDPI AG
2019-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/24/1/195 |
| _version_ | 1819105974273703936 |
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| author | Kem Ok Kim Dahae Lee Nguyen Tuan Hiep Ji Hoon Song Hae-Jeung Lee Dongho Lee Ki Sung Kang |
| author_facet | Kem Ok Kim Dahae Lee Nguyen Tuan Hiep Ji Hoon Song Hae-Jeung Lee Dongho Lee Ki Sung Kang |
| author_sort | Kem Ok Kim |
| collection | DOAJ |
| description | We investigated whether 14 phenolic compounds isolated from Artemisia argyi could prevent the apoptotic damage caused by iodixanol, an iodinated contrast agent, on LLC-PK1 cells. Iodixanol was used to induce cytotoxicity in LLC-PK1 cells. Apoptotic cell death was observed as the fluorescence intensity emitted by annexin V and Hoechst 33342 stains. Western blotting was used to detect specific proteins. Seven phenolic compounds protected against iodixanol-induced LLC-PK1 cell death in a concentration-dependent manner. Among them, methyl caffeate exerted the strongest protective effect, and co-treatment with 50 and 100 μM methyl caffeate decreased intracellular reactive oxygen species elevated by 25 mg/mL iodixanol. In addition, the treatment of LLC-PK1 cells with iodixanol resulted in an increase in apoptotic cell death, which decreased by co-treatment with methyl caffeate. Iodixanol caused a cytotoxicity-related increase in the phosphorylation of extracellular-signal-regulated kinase, c-Jun N-terminal kinase, and P38; and a similar increase in the expression levels of kidney injury molecule-1 and cleaved caspase-3. However, the up-regulation of these proteins was reversed by co-treatment with methyl caffeate. These findings suggest that phenolic compounds isolated from A. argyi play an important role in protecting kidney epithelium cells against apoptotic damage caused by iodixanol. |
| first_indexed | 2024-12-22T02:30:46Z |
| format | Article |
| id | doaj.art-35111af36ba745e4a9dd5faa5a920970 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T02:30:46Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-35111af36ba745e4a9dd5faa5a9209702022-12-21T18:41:53ZengMDPI AGMolecules1420-30492019-01-0124119510.3390/molecules24010195molecules24010195Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1Kem Ok Kim0Dahae Lee1Nguyen Tuan Hiep2Ji Hoon Song3Hae-Jeung Lee4Dongho Lee5Ki Sung Kang6Department of Biosystems and Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 440-746, KoreaDepartment of Biosystems and Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaDepartment of Food and Nutrition, College of Bio-Nano technology, Gachon University, Seongnam 13120, KoreaDepartment of Biosystems and Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaWe investigated whether 14 phenolic compounds isolated from Artemisia argyi could prevent the apoptotic damage caused by iodixanol, an iodinated contrast agent, on LLC-PK1 cells. Iodixanol was used to induce cytotoxicity in LLC-PK1 cells. Apoptotic cell death was observed as the fluorescence intensity emitted by annexin V and Hoechst 33342 stains. Western blotting was used to detect specific proteins. Seven phenolic compounds protected against iodixanol-induced LLC-PK1 cell death in a concentration-dependent manner. Among them, methyl caffeate exerted the strongest protective effect, and co-treatment with 50 and 100 μM methyl caffeate decreased intracellular reactive oxygen species elevated by 25 mg/mL iodixanol. In addition, the treatment of LLC-PK1 cells with iodixanol resulted in an increase in apoptotic cell death, which decreased by co-treatment with methyl caffeate. Iodixanol caused a cytotoxicity-related increase in the phosphorylation of extracellular-signal-regulated kinase, c-Jun N-terminal kinase, and P38; and a similar increase in the expression levels of kidney injury molecule-1 and cleaved caspase-3. However, the up-regulation of these proteins was reversed by co-treatment with methyl caffeate. These findings suggest that phenolic compounds isolated from A. argyi play an important role in protecting kidney epithelium cells against apoptotic damage caused by iodixanol.http://www.mdpi.com/1420-3049/24/1/195contrast agentiodixanolcytotoxicityMAPKscaspase-3kidney injury molecule-1 |
| spellingShingle | Kem Ok Kim Dahae Lee Nguyen Tuan Hiep Ji Hoon Song Hae-Jeung Lee Dongho Lee Ki Sung Kang Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1 Molecules contrast agent iodixanol cytotoxicity MAPKs caspase-3 kidney injury molecule-1 |
| title | Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1 |
| title_full | Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1 |
| title_fullStr | Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1 |
| title_full_unstemmed | Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1 |
| title_short | Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1 |
| title_sort | protective effect of phenolic compounds isolated from mugwort artemisia argyi against contrast induced apoptosis in kidney epithelium cell line llc pk1 |
| topic | contrast agent iodixanol cytotoxicity MAPKs caspase-3 kidney injury molecule-1 |
| url | http://www.mdpi.com/1420-3049/24/1/195 |
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