Association between polymorphisms of IL-33 and IL-37 and atopic dermatitis
Atopic dermatitis (AD) is a chronic recurrent inflammatory disease accompanied by severe itching. One of the leading mechanisms underlying the development of AD is an imbalance of the Th1/Th2 cells immune response, which leads to an increased production of inflammatory mediators, including IL-1 fami...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2019-08-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/2936 |
| _version_ | 1797197726698438656 |
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| author | Oxana A. Svitich Olga Yu. Olisova Mariia B. Potapova Ekaterina A. Meremianina Nadezhda D. Rasskazova Elizaveta A. Belokopytova Anna A. Solodkova Antonina G. Upatova |
| author_facet | Oxana A. Svitich Olga Yu. Olisova Mariia B. Potapova Ekaterina A. Meremianina Nadezhda D. Rasskazova Elizaveta A. Belokopytova Anna A. Solodkova Antonina G. Upatova |
| author_sort | Oxana A. Svitich |
| collection | DOAJ |
| description | Atopic dermatitis (AD) is a chronic recurrent inflammatory disease accompanied by severe itching. One of the leading mechanisms underlying the development of AD is an imbalance of the Th1/Th2 cells immune response, which leads to an increased production of inflammatory mediators, including IL-1 family. The IL-1 family includes the recently discovered IL-33 and IL-37 and its role in the pathogenesis of AD has been actively studied. IL-33 functions as an alarmin that can induce IL-31 production, thereby leading to skin barrier impairment, pruritus and scratching. Having both immunomodulatory and immunosuppressive properties, IL-37 suppresses leukocyte infiltration of the affected skin and reduces the activity of proinflammatory cytokines. The aim of the research was to find an association between polymorphisms in the genes encoding IL-33, IL-37 and risk of AD. A total of 98 patients with moderate and severe AD were included in the study. The control group included 72 healthy volunteers. Polymorphic markers were determined in peripheral blood. After total RNA extraction, polymorphic markers rs7019575 in the IL-33 gene, rs3811046 and rs3811047 in the IL-37 gene were analyzed using RT-PCR. There was no statistically significant difference in allele frequency and genotype distribution of rs7019575 (IL-33) and rs3811047 (IL-37). The research of the polymorphic marker rs3811046 in the IL-37 gene showed that the risk of AD was almost 2 times lower for the allele G and higher more than 2 times for TT homozygous carriers. The haplotype analysis revealed that the GTAA and TTGG haplotypes of IL-37 were associated with AD, increasing the risk of development by 2 and 10 times, respectively. In conclusion, SNP markers identified in this study can be used to predict the risk of developing AD in individuals with a positive family history of atopic diseases. |
| first_indexed | 2024-04-24T06:48:33Z |
| format | Article |
| id | doaj.art-351184a1abf040d199e36e0f8afbef42 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:48:33Z |
| publishDate | 2019-08-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-351184a1abf040d199e36e0f8afbef422024-04-22T13:07:52ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2019-08-010010.15789/1563-0625-ABP-29361870Association between polymorphisms of IL-33 and IL-37 and atopic dermatitisOxana A. Svitich0Olga Yu. Olisova1Mariia B. Potapova2Ekaterina A. Meremianina3Nadezhda D. Rasskazova4Elizaveta A. Belokopytova5Anna A. Solodkova6Antonina G. Upatova7I. I. Mechnikov Research Institute for Vaccines and Sera, I.M. Sechenov First Moscow State Medical UniversityI.M. Sechenov First Moscow State Medical UniversityI. I. Mechnikov Research Institute for Vaccines and SeraI. I. Mechnikov Research Institute for Vaccines and Sera, Russian Medical Academy of Continuous Professional EducationI. I. Mechnikov Research Institute for Vaccines and SeraI. I. Mechnikov Research Institute for Vaccines and SeraI. I. Mechnikov Research Institute for Vaccines and SeraI. I. Mechnikov Research Institute for Vaccines and SeraAtopic dermatitis (AD) is a chronic recurrent inflammatory disease accompanied by severe itching. One of the leading mechanisms underlying the development of AD is an imbalance of the Th1/Th2 cells immune response, which leads to an increased production of inflammatory mediators, including IL-1 family. The IL-1 family includes the recently discovered IL-33 and IL-37 and its role in the pathogenesis of AD has been actively studied. IL-33 functions as an alarmin that can induce IL-31 production, thereby leading to skin barrier impairment, pruritus and scratching. Having both immunomodulatory and immunosuppressive properties, IL-37 suppresses leukocyte infiltration of the affected skin and reduces the activity of proinflammatory cytokines. The aim of the research was to find an association between polymorphisms in the genes encoding IL-33, IL-37 and risk of AD. A total of 98 patients with moderate and severe AD were included in the study. The control group included 72 healthy volunteers. Polymorphic markers were determined in peripheral blood. After total RNA extraction, polymorphic markers rs7019575 in the IL-33 gene, rs3811046 and rs3811047 in the IL-37 gene were analyzed using RT-PCR. There was no statistically significant difference in allele frequency and genotype distribution of rs7019575 (IL-33) and rs3811047 (IL-37). The research of the polymorphic marker rs3811046 in the IL-37 gene showed that the risk of AD was almost 2 times lower for the allele G and higher more than 2 times for TT homozygous carriers. The haplotype analysis revealed that the GTAA and TTGG haplotypes of IL-37 were associated with AD, increasing the risk of development by 2 and 10 times, respectively. In conclusion, SNP markers identified in this study can be used to predict the risk of developing AD in individuals with a positive family history of atopic diseases.https://www.mimmun.ru/mimmun/article/view/2936atopic dermatitis, tlr, snp, marker, atopic diseases, innate immunity |
| spellingShingle | Oxana A. Svitich Olga Yu. Olisova Mariia B. Potapova Ekaterina A. Meremianina Nadezhda D. Rasskazova Elizaveta A. Belokopytova Anna A. Solodkova Antonina G. Upatova Association between polymorphisms of IL-33 and IL-37 and atopic dermatitis Медицинская иммунология atopic dermatitis, tlr, snp, marker, atopic diseases, innate immunity |
| title | Association between polymorphisms of IL-33 and IL-37 and atopic dermatitis |
| title_full | Association between polymorphisms of IL-33 and IL-37 and atopic dermatitis |
| title_fullStr | Association between polymorphisms of IL-33 and IL-37 and atopic dermatitis |
| title_full_unstemmed | Association between polymorphisms of IL-33 and IL-37 and atopic dermatitis |
| title_short | Association between polymorphisms of IL-33 and IL-37 and atopic dermatitis |
| title_sort | association between polymorphisms of il 33 and il 37 and atopic dermatitis |
| topic | atopic dermatitis, tlr, snp, marker, atopic diseases, innate immunity |
| url | https://www.mimmun.ru/mimmun/article/view/2936 |
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