Glycolytic reprogramming is involved in tissue remodeling on chronic rhinosinusitis.
<h4>Background</h4>Glycolytic reprogramming is a key feature of chronic inflammatory disease. Extracellular matrix (ECM) produced by myofibroblasts plays an important role in tissue remodeling of nasal mucosa in chronic rhinosinusitis (CRS). This study aimed to determine whether glycolyt...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0281640 |
| _version_ | 1797895467835588608 |
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| author | Min-Sik Jo Hyun-Woo Yang Joo-Hoo Park Jae-Min Shin Il-Ho Park |
| author_facet | Min-Sik Jo Hyun-Woo Yang Joo-Hoo Park Jae-Min Shin Il-Ho Park |
| author_sort | Min-Sik Jo |
| collection | DOAJ |
| description | <h4>Background</h4>Glycolytic reprogramming is a key feature of chronic inflammatory disease. Extracellular matrix (ECM) produced by myofibroblasts plays an important role in tissue remodeling of nasal mucosa in chronic rhinosinusitis (CRS). This study aimed to determine whether glycolytic reprogramming contributes to myofibroblast differentiation and ECM production in nasal fibroblasts.<h4>Methods</h4>Primary nasal fibroblasts were isolated from the nasal mucosa of patients with CRS. Glycolytic reprogramming was assessed by measuring the extracellular acidification and oxygen consumption rates in nasal fibroblast, with and without transforming growth factor beta 1 (TGF-β1) treatment. Expression of glycolytic enzymes and ECM components was measured by real-time polymerase chain reaction, western blotting, and immunocytochemical staining. Gene set enrichment analysis was performed using whole RNA-sequencing data of nasal mucosa of healthy donors and patients with CRS.<h4>Result</h4>Glycolysis of nasal fibroblasts stimulated with TGF-B1 was upregulated along with glycolytic enzymes. Hypoxia-inducing factor (HIF)-1α was a high-level regulator of glycolysis, and increased HIF-1α expression promoted glycolysis of nasal fibroblasts, and inhibition of HIF-1α down-regulated myofibroblasts differentiation and ECM production.<h4>Conclusion</h4>This study suggests that inhibition of the glycolytic enzyme and HIF-1α in nasal fibroblasts regulates myofibroblast differentiation and ECM generation associated with nasal mucosa remodeling. |
| first_indexed | 2024-04-10T07:26:12Z |
| format | Article |
| id | doaj.art-35158a169a824a2192ae2e98c6d528a0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T07:26:12Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-35158a169a824a2192ae2e98c6d528a02023-02-24T05:31:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01182e028164010.1371/journal.pone.0281640Glycolytic reprogramming is involved in tissue remodeling on chronic rhinosinusitis.Min-Sik JoHyun-Woo YangJoo-Hoo ParkJae-Min ShinIl-Ho Park<h4>Background</h4>Glycolytic reprogramming is a key feature of chronic inflammatory disease. Extracellular matrix (ECM) produced by myofibroblasts plays an important role in tissue remodeling of nasal mucosa in chronic rhinosinusitis (CRS). This study aimed to determine whether glycolytic reprogramming contributes to myofibroblast differentiation and ECM production in nasal fibroblasts.<h4>Methods</h4>Primary nasal fibroblasts were isolated from the nasal mucosa of patients with CRS. Glycolytic reprogramming was assessed by measuring the extracellular acidification and oxygen consumption rates in nasal fibroblast, with and without transforming growth factor beta 1 (TGF-β1) treatment. Expression of glycolytic enzymes and ECM components was measured by real-time polymerase chain reaction, western blotting, and immunocytochemical staining. Gene set enrichment analysis was performed using whole RNA-sequencing data of nasal mucosa of healthy donors and patients with CRS.<h4>Result</h4>Glycolysis of nasal fibroblasts stimulated with TGF-B1 was upregulated along with glycolytic enzymes. Hypoxia-inducing factor (HIF)-1α was a high-level regulator of glycolysis, and increased HIF-1α expression promoted glycolysis of nasal fibroblasts, and inhibition of HIF-1α down-regulated myofibroblasts differentiation and ECM production.<h4>Conclusion</h4>This study suggests that inhibition of the glycolytic enzyme and HIF-1α in nasal fibroblasts regulates myofibroblast differentiation and ECM generation associated with nasal mucosa remodeling.https://doi.org/10.1371/journal.pone.0281640 |
| spellingShingle | Min-Sik Jo Hyun-Woo Yang Joo-Hoo Park Jae-Min Shin Il-Ho Park Glycolytic reprogramming is involved in tissue remodeling on chronic rhinosinusitis. PLoS ONE |
| title | Glycolytic reprogramming is involved in tissue remodeling on chronic rhinosinusitis. |
| title_full | Glycolytic reprogramming is involved in tissue remodeling on chronic rhinosinusitis. |
| title_fullStr | Glycolytic reprogramming is involved in tissue remodeling on chronic rhinosinusitis. |
| title_full_unstemmed | Glycolytic reprogramming is involved in tissue remodeling on chronic rhinosinusitis. |
| title_short | Glycolytic reprogramming is involved in tissue remodeling on chronic rhinosinusitis. |
| title_sort | glycolytic reprogramming is involved in tissue remodeling on chronic rhinosinusitis |
| url | https://doi.org/10.1371/journal.pone.0281640 |
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