Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritis
Background. Escherichia coli enteritis is one of the most common causes of diarrhea in developed countries and is caused usually by pathogenic strains of Escherichia coli. Objectives. To investigate the role of reactive response of polymorphonuclear neutrophilic granulocytes (NG) of peripheral bl...
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| Format: | Article |
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Balkan Medical Union
2019-03-01
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| Series: | Archives of the Balkan Medical Union |
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| Online Access: | https://umbalk.org/wp-content/uploads/2019/03/12.CLINICAL-MARKERS-OF-IMMUNE-DISORDERS.pdf |
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| author | Larysa P. SYDORCHUK1 Boris V. SYROTA1 Andrii R. SYDORCHUK Oleg V. GERUSH Natalia Y. MUZYKA Michael I. SHEREMET Oksana M. KOROVENKOVA Natalia S. BOHDAN Olha Y. SKRYNCHUK Liviya V. NIKYFOR Oksana M. IFTODA Serhiy I. IVASHCHUK Oksana V. KUSHNIR |
| author_facet | Larysa P. SYDORCHUK1 Boris V. SYROTA1 Andrii R. SYDORCHUK Oleg V. GERUSH Natalia Y. MUZYKA Michael I. SHEREMET Oksana M. KOROVENKOVA Natalia S. BOHDAN Olha Y. SKRYNCHUK Liviya V. NIKYFOR Oksana M. IFTODA Serhiy I. IVASHCHUK Oksana V. KUSHNIR |
| author_sort | Larysa P. SYDORCHUK1 |
| collection | DOAJ |
| description | Background. Escherichia coli enteritis is one of the most common causes of diarrhea in developed countries and is caused usually by pathogenic strains of Escherichia coli.
Objectives. To investigate the role of reactive response of polymorphonuclear neutrophilic granulocytes (NG) of peripheral blood in the systemic inflammatory response mechanisms of acute Escherichia enterocolitis (AEC), depending on genes polymorphism of heat shock proteins (HSP) family 70-2 (HSP70-2, 1267A®G) and interleukin 10 (IL-10, C-592A).
Material and methods. The genes polymorphism was analysed by PCR based method in 95 patients with AEC and 30 healthy individuals. Clinical markers of immune disorders were evaluated after hematological indices, based upon an extended general clinical blood analysis, using verified formulas.
Results. The endogenous intoxication severity did not depend reliably on genotypes of IL-10 gene (rs1800872), however it was significantly 23.68% (р=0.043) higher in GG-genotype carriers of HSP70-2 gene (rs1061581). The reduction of cellular reactivity by 14.71-19.08% (р<0.01) did not depend on the analyzed genes genotypes. But general non-specific immune reactivity decreases 3.49-4.24 times (р<0.001) was deeper in GG-genotype carriers of HSP70-2 gene and AA-genotype carriers of IL-10 gene by 17.78% (р=0.009) and 12.37% (р=0.023) respectively. The immunologic resistance index was lower by 18.75% (р=0.024) in GG-genotype carriers than in patients with А allele.
Conclusions. Hematological indices, based upon an extended general clinical blood analysis, are indicative and reliable non-specific clinical markers of immune disorders in case of AEC. |
| first_indexed | 2024-12-14T13:15:07Z |
| format | Article |
| id | doaj.art-3518c08367464c499c3115ef13b4f1db |
| institution | Directory Open Access Journal |
| issn | 1584-9244 2558-815X |
| language | English |
| last_indexed | 2024-12-14T13:15:07Z |
| publishDate | 2019-03-01 |
| publisher | Balkan Medical Union |
| record_format | Article |
| series | Archives of the Balkan Medical Union |
| spelling | doaj.art-3518c08367464c499c3115ef13b4f1db2022-12-21T23:00:04ZengBalkan Medical UnionArchives of the Balkan Medical Union1584-92442558-815X2019-03-01541899610.31688/ABMU.2019.54.1.12Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritisLarysa P. SYDORCHUK1 0 Boris V. SYROTA11Andrii R. SYDORCHUK2Oleg V. GERUSH3Natalia Y. MUZYKA4Michael I. SHEREMET5 Oksana M. KOROVENKOVA6Natalia S. BOHDAN7Olha Y. SKRYNCHUK8Liviya V. NIKYFOR9Oksana M. IFTODA10Serhiy I. IVASHCHUK11 Oksana V. KUSHNIR12 Department of Family Medicine, Higher State Educational Establishment of Ukraine «Bukovinian State Medical University» (BSMU), Ukraine Department of Family Medicine, Higher State Educational Establishment of Ukraine «Bukovinian State Medical University» (BSMU), Ukraine Department of Family Medicine, Higher State Educational Establishment of Ukraine «Bukovinian State Medical University» (BSMU), Ukraine Pharmacology Department, BSMU, Ukraine Pharmacology Department, BSMU, Ukraine Surgery Department No 1, BSMU, Ukraine Pharmacology Department, BSMU, Ukraine Pharmacology Department, BSMU, Ukraine Pharmacology Department, BSMU, Ukraine Department of Family Medicine, Higher State Educational Establishment of Ukraine «Bukovinian State Medical University» (BSMU), Ukraine Hygiene and Ecology Department, BSMU, Ukraine Department of Family Medicine, Higher State Educational Establishment of Ukraine «Bukovinian State Medical University» (BSMU), Ukraine Hygiene and Ecology Department, BSMU, UkraineBackground. Escherichia coli enteritis is one of the most common causes of diarrhea in developed countries and is caused usually by pathogenic strains of Escherichia coli. Objectives. To investigate the role of reactive response of polymorphonuclear neutrophilic granulocytes (NG) of peripheral blood in the systemic inflammatory response mechanisms of acute Escherichia enterocolitis (AEC), depending on genes polymorphism of heat shock proteins (HSP) family 70-2 (HSP70-2, 1267A®G) and interleukin 10 (IL-10, C-592A). Material and methods. The genes polymorphism was analysed by PCR based method in 95 patients with AEC and 30 healthy individuals. Clinical markers of immune disorders were evaluated after hematological indices, based upon an extended general clinical blood analysis, using verified formulas. Results. The endogenous intoxication severity did not depend reliably on genotypes of IL-10 gene (rs1800872), however it was significantly 23.68% (р=0.043) higher in GG-genotype carriers of HSP70-2 gene (rs1061581). The reduction of cellular reactivity by 14.71-19.08% (р<0.01) did not depend on the analyzed genes genotypes. But general non-specific immune reactivity decreases 3.49-4.24 times (р<0.001) was deeper in GG-genotype carriers of HSP70-2 gene and AA-genotype carriers of IL-10 gene by 17.78% (р=0.009) and 12.37% (р=0.023) respectively. The immunologic resistance index was lower by 18.75% (р=0.024) in GG-genotype carriers than in patients with А allele. Conclusions. Hematological indices, based upon an extended general clinical blood analysis, are indicative and reliable non-specific clinical markers of immune disorders in case of AEC.https://umbalk.org/wp-content/uploads/2019/03/12.CLINICAL-MARKERS-OF-IMMUNE-DISORDERS.pdfmarkersimmune disordersgenes IL-10 (rs1800872)HSP70-2 (rs1061581)E. coli enteritis |
| spellingShingle | Larysa P. SYDORCHUK1 Boris V. SYROTA1 Andrii R. SYDORCHUK Oleg V. GERUSH Natalia Y. MUZYKA Michael I. SHEREMET Oksana M. KOROVENKOVA Natalia S. BOHDAN Olha Y. SKRYNCHUK Liviya V. NIKYFOR Oksana M. IFTODA Serhiy I. IVASHCHUK Oksana V. KUSHNIR Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritis Archives of the Balkan Medical Union markers immune disorders genes IL-10 (rs1800872) HSP70-2 (rs1061581) E. coli enteritis |
| title | Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritis |
| title_full | Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritis |
| title_fullStr | Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritis |
| title_full_unstemmed | Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritis |
| title_short | Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritis |
| title_sort | clinical markers of immune disorders in the pathogenesis of escherichia coli enteritis |
| topic | markers immune disorders genes IL-10 (rs1800872) HSP70-2 (rs1061581) E. coli enteritis |
| url | https://umbalk.org/wp-content/uploads/2019/03/12.CLINICAL-MARKERS-OF-IMMUNE-DISORDERS.pdf |
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