Integrated Metabolomics and Transcriptomics Analyses Reveal the Candidate Genes Regulating the Meat Quality Change by Castration in Yudong Black Goats (<i>Capra hircus</i>)
Yudong black goats (YDGs) are a local breed in southwest China that possess unique meat qualities and produce a high meat yield, making them ideal models for studying goat meat quality. Castration may decrease off-odors, significantly change metabolites and improve meat quality. Using multi-omics te...
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MDPI AG
2023-12-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/15/1/43 |
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| author | Songjian Yang Xinyue Zhang Xingchun Li Jikang Zheng Le Zhao Chengli Fan Yongju Zhao |
| author_facet | Songjian Yang Xinyue Zhang Xingchun Li Jikang Zheng Le Zhao Chengli Fan Yongju Zhao |
| author_sort | Songjian Yang |
| collection | DOAJ |
| description | Yudong black goats (YDGs) are a local breed in southwest China that possess unique meat qualities and produce a high meat yield, making them ideal models for studying goat meat quality. Castration may decrease off-odors, significantly change metabolites and improve meat quality. Using multi-omics techniques, this study focused on Yudong black goat wethers (YDW, n = 4) and Yudong black bucks (YDB, n = 4). The findings revealed that 33 differentially expressed genes (DEGs) and 279 significantly changed metabolites (SCMs) influenced goat meat quality by affecting fat accumulation and lipolysis regulatory processes. Herein, several candidate genes (<i>IGF1</i>, <i>TNNT2</i>, <i>PPP2R2C</i>, <i>MAPK10</i> and <i>VNN1</i>, etc.) were identified that play a role in regulating meat quality, non-castrated and castrated, alongside a series of metabolites that may serve as potential meat quality biomarkers. Lipids (triglycerides, oxidized lipids_5-iso PGF2VI, ceramide (t18:1/36:2(2OH)) and Carnitine C20:5, etc.) were significantly higher in the castrated goats. These results revealed that lipids and hydrophilic metabolites were affected by castration, which might be beneficial in terms of goat meat quality. This study aimed to investigate the differences in meat quality between uncastrated and castrated male goats and the possible molecular regulatory mechanisms. |
| first_indexed | 2024-03-08T10:54:04Z |
| format | Article |
| id | doaj.art-3519233546ff4b059986221800d50e6e |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-08T10:54:04Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-3519233546ff4b059986221800d50e6e2024-01-26T16:41:38ZengMDPI AGGenes2073-44252023-12-011514310.3390/genes15010043Integrated Metabolomics and Transcriptomics Analyses Reveal the Candidate Genes Regulating the Meat Quality Change by Castration in Yudong Black Goats (<i>Capra hircus</i>)Songjian Yang0Xinyue Zhang1Xingchun Li2Jikang Zheng3Le Zhao4Chengli Fan5Yongju Zhao6Chongqing Key Laboratory of Forage & Herbivore, Chongqing 400715, ChinaChongqing Key Laboratory of Forage & Herbivore, Chongqing 400715, ChinaChongqing Key Laboratory of Forage & Herbivore, Chongqing 400715, ChinaChongqing Key Laboratory of Forage & Herbivore, Chongqing 400715, ChinaChongqing Key Laboratory of Forage & Herbivore, Chongqing 400715, ChinaChongqing Key Laboratory of Forage & Herbivore, Chongqing 400715, ChinaChongqing Key Laboratory of Forage & Herbivore, Chongqing 400715, ChinaYudong black goats (YDGs) are a local breed in southwest China that possess unique meat qualities and produce a high meat yield, making them ideal models for studying goat meat quality. Castration may decrease off-odors, significantly change metabolites and improve meat quality. Using multi-omics techniques, this study focused on Yudong black goat wethers (YDW, n = 4) and Yudong black bucks (YDB, n = 4). The findings revealed that 33 differentially expressed genes (DEGs) and 279 significantly changed metabolites (SCMs) influenced goat meat quality by affecting fat accumulation and lipolysis regulatory processes. Herein, several candidate genes (<i>IGF1</i>, <i>TNNT2</i>, <i>PPP2R2C</i>, <i>MAPK10</i> and <i>VNN1</i>, etc.) were identified that play a role in regulating meat quality, non-castrated and castrated, alongside a series of metabolites that may serve as potential meat quality biomarkers. Lipids (triglycerides, oxidized lipids_5-iso PGF2VI, ceramide (t18:1/36:2(2OH)) and Carnitine C20:5, etc.) were significantly higher in the castrated goats. These results revealed that lipids and hydrophilic metabolites were affected by castration, which might be beneficial in terms of goat meat quality. This study aimed to investigate the differences in meat quality between uncastrated and castrated male goats and the possible molecular regulatory mechanisms.https://www.mdpi.com/2073-4425/15/1/43goatmeatmetabolomicstranscriptomicscastration |
| spellingShingle | Songjian Yang Xinyue Zhang Xingchun Li Jikang Zheng Le Zhao Chengli Fan Yongju Zhao Integrated Metabolomics and Transcriptomics Analyses Reveal the Candidate Genes Regulating the Meat Quality Change by Castration in Yudong Black Goats (<i>Capra hircus</i>) Genes goat meat metabolomics transcriptomics castration |
| title | Integrated Metabolomics and Transcriptomics Analyses Reveal the Candidate Genes Regulating the Meat Quality Change by Castration in Yudong Black Goats (<i>Capra hircus</i>) |
| title_full | Integrated Metabolomics and Transcriptomics Analyses Reveal the Candidate Genes Regulating the Meat Quality Change by Castration in Yudong Black Goats (<i>Capra hircus</i>) |
| title_fullStr | Integrated Metabolomics and Transcriptomics Analyses Reveal the Candidate Genes Regulating the Meat Quality Change by Castration in Yudong Black Goats (<i>Capra hircus</i>) |
| title_full_unstemmed | Integrated Metabolomics and Transcriptomics Analyses Reveal the Candidate Genes Regulating the Meat Quality Change by Castration in Yudong Black Goats (<i>Capra hircus</i>) |
| title_short | Integrated Metabolomics and Transcriptomics Analyses Reveal the Candidate Genes Regulating the Meat Quality Change by Castration in Yudong Black Goats (<i>Capra hircus</i>) |
| title_sort | integrated metabolomics and transcriptomics analyses reveal the candidate genes regulating the meat quality change by castration in yudong black goats i capra hircus i |
| topic | goat meat metabolomics transcriptomics castration |
| url | https://www.mdpi.com/2073-4425/15/1/43 |
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