Optimized protocol to detect protein UFMylation in cells and in vitro via immunoblotting

Optimized protocol to detect protein UFMylation in cells and in vitro via immunoblotting

Summary: Ubiquitin-fold modifier 1 (UFM1) system is a recently identified ubiquitin-like modification with essential biological functions. Similar to ubiquitination, the covalent conjugation of UFM1 (UFMylation) to target proteins involves a three-step enzymatic cascade catalyzed sequentially by UFM...

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Bibliographic Details
Main Authors: Junzhi Zhou, Qian Liang, Maogong Dong, Xiaohe Ma, Yaqi Jin, Di Guan, Jiang Liu, Miao Wang, Yu-sheng Cong
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Molecular Biology
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007802

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