Cryptic species of Paracoccidioides brasiliensis: impact on paracoccidioidomycosis immunodiagnosis
We aimed to evaluate whether the occurrence of cryptic species of Paracoccidioides brasiliensis, S1, PS2, PS3 and Paracoccidioides lutzii, has implications in the immunodiagnosis of paracoccidioidomycosis (PCM). Small quantities of the antigen gp43 were found in culture filtrates of P. lutzii strain...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2013-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500637&lng=en&tlng=en |
| _version_ | 1797756562035441664 |
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| author | Gabriel Capella Machado Daniela Vanessa Moris Thales Domingos Arantes Luciane Regina Franciscone Silva Raquel Cordeiro Theodoro Rinaldo Pôncio Mendes Adriana Pardini Vicentini Eduardo Bagagli |
| author_facet | Gabriel Capella Machado Daniela Vanessa Moris Thales Domingos Arantes Luciane Regina Franciscone Silva Raquel Cordeiro Theodoro Rinaldo Pôncio Mendes Adriana Pardini Vicentini Eduardo Bagagli |
| author_sort | Gabriel Capella Machado |
| collection | DOAJ |
| description | We aimed to evaluate whether the occurrence of cryptic species of Paracoccidioides brasiliensis, S1, PS2, PS3 and Paracoccidioides lutzii, has implications in the immunodiagnosis of paracoccidioidomycosis (PCM). Small quantities of the antigen gp43 were found in culture filtrates of P. lutzii strains and this molecule appeared to be more variable within P. lutzii because the synonymous-nonsynonymous mutation rate was lower, indicating an evolutionary process different from that of the remaining genotypes. The production of gp43 also varied between isolates belonging to the same species, indicating that speciation events are important, but not sufficient to fully explain the diversity in the production of this antigen. The culture filtrate antigen AgEpm83, which was obtained from a PS3 isolate, showed large quantities of gp43 and reactivity by immunodiffusion assays, similar to the standard antigen (AgB-339) from an S1 isolate. Furthermore, AgEpm83 was capable of serologically differentiating five serum samples from patients from the Botucatu and Jundiaí regions. These patients had confirmed PCM but, were non-reactive to the standard antigen, thus demonstrating an alternative for serological diagnosis in regions in which S1 and PS2 occur. We also emphasise that it is not advisable to use a single antigen preparation to diagnose PCM, a disease that is caused by highly diverse pathogens. |
| first_indexed | 2024-03-12T18:03:17Z |
| format | Article |
| id | doaj.art-3519d8d9e0d24c59b0cbdbf9988630cb |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:03:17Z |
| publishDate | 2013-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-3519d8d9e0d24c59b0cbdbf9988630cb2023-08-02T09:32:29ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602013-08-01108563764310.1590/0074-0276108052013016S0074-02762013000500637Cryptic species of Paracoccidioides brasiliensis: impact on paracoccidioidomycosis immunodiagnosisGabriel Capella MachadoDaniela Vanessa MorisThales Domingos ArantesLuciane Regina Franciscone SilvaRaquel Cordeiro TheodoroRinaldo Pôncio MendesAdriana Pardini VicentiniEduardo BagagliWe aimed to evaluate whether the occurrence of cryptic species of Paracoccidioides brasiliensis, S1, PS2, PS3 and Paracoccidioides lutzii, has implications in the immunodiagnosis of paracoccidioidomycosis (PCM). Small quantities of the antigen gp43 were found in culture filtrates of P. lutzii strains and this molecule appeared to be more variable within P. lutzii because the synonymous-nonsynonymous mutation rate was lower, indicating an evolutionary process different from that of the remaining genotypes. The production of gp43 also varied between isolates belonging to the same species, indicating that speciation events are important, but not sufficient to fully explain the diversity in the production of this antigen. The culture filtrate antigen AgEpm83, which was obtained from a PS3 isolate, showed large quantities of gp43 and reactivity by immunodiffusion assays, similar to the standard antigen (AgB-339) from an S1 isolate. Furthermore, AgEpm83 was capable of serologically differentiating five serum samples from patients from the Botucatu and Jundiaí regions. These patients had confirmed PCM but, were non-reactive to the standard antigen, thus demonstrating an alternative for serological diagnosis in regions in which S1 and PS2 occur. We also emphasise that it is not advisable to use a single antigen preparation to diagnose PCM, a disease that is caused by highly diverse pathogens.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500637&lng=en&tlng=enParacoccidioides sppcryptic speciesantigen preparationsimmunodiagnosisgp43 |
| spellingShingle | Gabriel Capella Machado Daniela Vanessa Moris Thales Domingos Arantes Luciane Regina Franciscone Silva Raquel Cordeiro Theodoro Rinaldo Pôncio Mendes Adriana Pardini Vicentini Eduardo Bagagli Cryptic species of Paracoccidioides brasiliensis: impact on paracoccidioidomycosis immunodiagnosis Memorias do Instituto Oswaldo Cruz Paracoccidioides spp cryptic species antigen preparations immunodiagnosis gp43 |
| title | Cryptic species of Paracoccidioides brasiliensis: impact on paracoccidioidomycosis immunodiagnosis |
| title_full | Cryptic species of Paracoccidioides brasiliensis: impact on paracoccidioidomycosis immunodiagnosis |
| title_fullStr | Cryptic species of Paracoccidioides brasiliensis: impact on paracoccidioidomycosis immunodiagnosis |
| title_full_unstemmed | Cryptic species of Paracoccidioides brasiliensis: impact on paracoccidioidomycosis immunodiagnosis |
| title_short | Cryptic species of Paracoccidioides brasiliensis: impact on paracoccidioidomycosis immunodiagnosis |
| title_sort | cryptic species of paracoccidioides brasiliensis impact on paracoccidioidomycosis immunodiagnosis |
| topic | Paracoccidioides spp cryptic species antigen preparations immunodiagnosis gp43 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500637&lng=en&tlng=en |
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