<it>Salmonella</it> Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages
<p>Abstract</p> <p>Foodborne salmonellosis is one of the most important bacterial zoonotic diseases worldwide. <it>Salmonella</it> Typhimurium is the serovar most frequently isolated from persistently infected slaughter pigs in Europe. Circumvention of the host’s immune...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-06-01
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| Series: | Veterinary Research |
| Online Access: | http://www.veterinaryresearch.org/content/43/1/52 |
| _version_ | 1811260845018054656 |
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| author | Van Parys Alexander Boyen Filip Verbrugghe Elin Leyman Bregje Bram Flahou Haesebrouck Freddy Pasmans Frank |
| author_facet | Van Parys Alexander Boyen Filip Verbrugghe Elin Leyman Bregje Bram Flahou Haesebrouck Freddy Pasmans Frank |
| author_sort | Van Parys Alexander |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Foodborne salmonellosis is one of the most important bacterial zoonotic diseases worldwide. <it>Salmonella</it> Typhimurium is the serovar most frequently isolated from persistently infected slaughter pigs in Europe. Circumvention of the host’s immune system by <it>Salmonella</it> might contribute to persistent infection of pigs. In the present study, we found that <it>Salmonella</it> Typhimurium strain 112910a specifically downregulated MHC II, but not MHC I, expression on porcine alveolar macrophages in a <it>Salmonella</it> pathogenicity island (SPI)-1 and SPI-2 dependent way. <it>Salmonella</it> induced downregulation of MHC II expression and intracellular proliferation of <it>Salmonella</it> in macrophages were significantly impaired after opsonization with <it>Salmonella</it> specific antibodies prior to inoculation. Furthermore, the capacity to downregulate MHC II expression on macrophages differed significantly among <it>Salmonella</it> strains, independently of strain specific differences in invasion capacity, <it>Salmonella</it> induced cytotoxicity and altered macrophage activation status. The fact that strain specific differences in MHC II downregulation did not correlate with the extent of <it>in vitro</it> SPI-1 or SPI-2 gene expression indicates that other factors are involved in MHC II downregulation as well. Since <it>Salmonella</it> strain dependent interference with the pig’s immune response through downregulation of MHC II expression might indicate that certain <it>Salmonella</it> strains are more likely to escape serological detection, our findings are of major interest for <it>Salmonella</it> monitoring programs primarily based on serology.</p> |
| first_indexed | 2024-04-12T18:53:46Z |
| format | Article |
| id | doaj.art-351b157b1c534892a8508dda5d27e6d4 |
| institution | Directory Open Access Journal |
| issn | 0928-4249 1297-9716 |
| language | English |
| last_indexed | 2024-04-12T18:53:46Z |
| publishDate | 2012-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-351b157b1c534892a8508dda5d27e6d42022-12-22T03:20:24ZengBMCVeterinary Research0928-42491297-97162012-06-014315210.1186/1297-9716-43-52<it>Salmonella</it> Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophagesVan Parys AlexanderBoyen FilipVerbrugghe ElinLeyman BregjeBram FlahouHaesebrouck FreddyPasmans Frank<p>Abstract</p> <p>Foodborne salmonellosis is one of the most important bacterial zoonotic diseases worldwide. <it>Salmonella</it> Typhimurium is the serovar most frequently isolated from persistently infected slaughter pigs in Europe. Circumvention of the host’s immune system by <it>Salmonella</it> might contribute to persistent infection of pigs. In the present study, we found that <it>Salmonella</it> Typhimurium strain 112910a specifically downregulated MHC II, but not MHC I, expression on porcine alveolar macrophages in a <it>Salmonella</it> pathogenicity island (SPI)-1 and SPI-2 dependent way. <it>Salmonella</it> induced downregulation of MHC II expression and intracellular proliferation of <it>Salmonella</it> in macrophages were significantly impaired after opsonization with <it>Salmonella</it> specific antibodies prior to inoculation. Furthermore, the capacity to downregulate MHC II expression on macrophages differed significantly among <it>Salmonella</it> strains, independently of strain specific differences in invasion capacity, <it>Salmonella</it> induced cytotoxicity and altered macrophage activation status. The fact that strain specific differences in MHC II downregulation did not correlate with the extent of <it>in vitro</it> SPI-1 or SPI-2 gene expression indicates that other factors are involved in MHC II downregulation as well. Since <it>Salmonella</it> strain dependent interference with the pig’s immune response through downregulation of MHC II expression might indicate that certain <it>Salmonella</it> strains are more likely to escape serological detection, our findings are of major interest for <it>Salmonella</it> monitoring programs primarily based on serology.</p>http://www.veterinaryresearch.org/content/43/1/52 |
| spellingShingle | Van Parys Alexander Boyen Filip Verbrugghe Elin Leyman Bregje Bram Flahou Haesebrouck Freddy Pasmans Frank <it>Salmonella</it> Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages Veterinary Research |
| title | <it>Salmonella</it> Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages |
| title_full | <it>Salmonella</it> Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages |
| title_fullStr | <it>Salmonella</it> Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages |
| title_full_unstemmed | <it>Salmonella</it> Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages |
| title_short | <it>Salmonella</it> Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages |
| title_sort | it salmonella it typhimurium induces spi 1 and spi 2 regulated and strain dependent downregulation of mhc ii expression on porcine alveolar macrophages |
| url | http://www.veterinaryresearch.org/content/43/1/52 |
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