Investigation of VEGF gene polymorphism rs35569394 in endometriosis
ABSTRACT Introduction: Endometriosis is a chronic gynecological condition characterized by the presence of endometrial tissue in locations outside the uterus. Its symptoms include dysmenorrhea, dyspareunia, chronic pelvic pain, infertility, dysuria, and dyschezia. Several studies have related the v...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Patologia Clínica
2018-12-01
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| Series: | Jornal Brasileiro de Patologia e Medicina Laboratorial |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442018000600359&tlng=en |
| _version_ | 1819258965448458240 |
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| author | Lysandra T. Bruno Marco Fábio Prata-Lima Mariangela T. Ruiz-Cintra Alessandra Bernadete Trovó de Marqui |
| author_facet | Lysandra T. Bruno Marco Fábio Prata-Lima Mariangela T. Ruiz-Cintra Alessandra Bernadete Trovó de Marqui |
| author_sort | Lysandra T. Bruno |
| collection | DOAJ |
| description | ABSTRACT Introduction: Endometriosis is a chronic gynecological condition characterized by the presence of endometrial tissue in locations outside the uterus. Its symptoms include dysmenorrhea, dyspareunia, chronic pelvic pain, infertility, dysuria, and dyschezia. Several studies have related the vascular endothelial growth factor (VEGF) gene polymorphisms as a factor for the development of endometriosis. Objectives: The aim of this study was to compare the frequency of the VEGF gene polymorphism rs35569394 in women with endometriosis and a control group, as well as to investigate the association of this polymorphism with the risk of endometriosis. Methods: This case-control study included 45 endometriosis patients (cases) and 78 controls. The molecular analysis was performed by polymerase chain reaction (PCR). The chi-square test was used to compare the genotype and allele frequencies, and the Hardy-Weinberg equilibrium was also tested using the chi-square test, considering the significance of p < 0.05. Results: The frequency of the II, ID and DD genotype was 24.4% versus 26.9%; 35.6% versus 37.2%; and 40% versus 35.9%, in patients and the control group, respectively. The allele frequencies were I: 0.42 (cases) and 0.45 (control); D: 0.58 (cases) and 0.55 (control). The allele and genotype frequency of VEGF -2549I/D polymorphism was not different between women with endometriosis and the control. Conclusion: In the present study, the frequency of the VEGF gene polymorphism rs35569394 is similar between cases and control. This genetic variant is not associated with endometriosis in the investigated population. |
| first_indexed | 2024-12-23T19:02:30Z |
| format | Article |
| id | doaj.art-35219c7c24b346609942bfe11b93dc77 |
| institution | Directory Open Access Journal |
| issn | 1678-4774 |
| language | English |
| last_indexed | 2024-12-23T19:02:30Z |
| publishDate | 2018-12-01 |
| publisher | Sociedade Brasileira de Patologia Clínica |
| record_format | Article |
| series | Jornal Brasileiro de Patologia e Medicina Laboratorial |
| spelling | doaj.art-35219c7c24b346609942bfe11b93dc772022-12-21T17:34:40ZengSociedade Brasileira de Patologia ClínicaJornal Brasileiro de Patologia e Medicina Laboratorial1678-47742018-12-0154635936310.5935/1676-2444.20180057Investigation of VEGF gene polymorphism rs35569394 in endometriosisLysandra T. BrunoMarco Fábio Prata-LimaMariangela T. Ruiz-CintraAlessandra Bernadete Trovó de MarquiABSTRACT Introduction: Endometriosis is a chronic gynecological condition characterized by the presence of endometrial tissue in locations outside the uterus. Its symptoms include dysmenorrhea, dyspareunia, chronic pelvic pain, infertility, dysuria, and dyschezia. Several studies have related the vascular endothelial growth factor (VEGF) gene polymorphisms as a factor for the development of endometriosis. Objectives: The aim of this study was to compare the frequency of the VEGF gene polymorphism rs35569394 in women with endometriosis and a control group, as well as to investigate the association of this polymorphism with the risk of endometriosis. Methods: This case-control study included 45 endometriosis patients (cases) and 78 controls. The molecular analysis was performed by polymerase chain reaction (PCR). The chi-square test was used to compare the genotype and allele frequencies, and the Hardy-Weinberg equilibrium was also tested using the chi-square test, considering the significance of p < 0.05. Results: The frequency of the II, ID and DD genotype was 24.4% versus 26.9%; 35.6% versus 37.2%; and 40% versus 35.9%, in patients and the control group, respectively. The allele frequencies were I: 0.42 (cases) and 0.45 (control); D: 0.58 (cases) and 0.55 (control). The allele and genotype frequency of VEGF -2549I/D polymorphism was not different between women with endometriosis and the control. Conclusion: In the present study, the frequency of the VEGF gene polymorphism rs35569394 is similar between cases and control. This genetic variant is not associated with endometriosis in the investigated population.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442018000600359&tlng=enendometriosisgenetic polymorphismpolymerase chain reaction |
| spellingShingle | Lysandra T. Bruno Marco Fábio Prata-Lima Mariangela T. Ruiz-Cintra Alessandra Bernadete Trovó de Marqui Investigation of VEGF gene polymorphism rs35569394 in endometriosis Jornal Brasileiro de Patologia e Medicina Laboratorial endometriosis genetic polymorphism polymerase chain reaction |
| title | Investigation of VEGF gene polymorphism rs35569394 in endometriosis |
| title_full | Investigation of VEGF gene polymorphism rs35569394 in endometriosis |
| title_fullStr | Investigation of VEGF gene polymorphism rs35569394 in endometriosis |
| title_full_unstemmed | Investigation of VEGF gene polymorphism rs35569394 in endometriosis |
| title_short | Investigation of VEGF gene polymorphism rs35569394 in endometriosis |
| title_sort | investigation of vegf gene polymorphism rs35569394 in endometriosis |
| topic | endometriosis genetic polymorphism polymerase chain reaction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442018000600359&tlng=en |
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