Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and <i>Filipendula ulmaria</i> Extract in Rats
The aim of this study was to evaluate the alterations of the hippocampal function that may be related to anxiogenic response to thermal skin injury, including the morpho-functional alterations, and the effects of hyperbaric oxygen (HBO) and <i>Filipendula ulmaria</i> (FU) extract in the...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/3033 |
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| author | Bojana Krstic Dragica Selakovic Nemanja Jovicic Milos Krstic Jelena S. Katanic Stankovic Sara Rosic Dragan Milovanovic Gvozden Rosic |
| author_facet | Bojana Krstic Dragica Selakovic Nemanja Jovicic Milos Krstic Jelena S. Katanic Stankovic Sara Rosic Dragan Milovanovic Gvozden Rosic |
| author_sort | Bojana Krstic |
| collection | DOAJ |
| description | The aim of this study was to evaluate the alterations of the hippocampal function that may be related to anxiogenic response to thermal skin injury, including the morpho-functional alterations, and the effects of hyperbaric oxygen (HBO) and <i>Filipendula ulmaria</i> (FU) extract in the treatment of anxiety-like behavior that coincides with thermal skin injury. A rat thermal skin injury experimental model was performed on 2-month-old male <i>Wistar albino</i> rats. The evaluated therapeutic protocols included HBO and/or antioxidant supplementation. HBO was applied for 7 days in the hyperbaric chamber (100% O<sub>2</sub>, 2.5 ATA, 60 min). Oral administration of FU extract (final concentration of 100 mg/kg b.w.) to achieve antioxidant supplementation was also applied for 7 days. Anxiety level was estimated in the open field and elevated plus-maze test, which was followed by anesthesia, sacrifice, and collection of hippocampal tissue samples. HBO treatment and FU supplementation significantly abolished anxiogenic response to thermal skin injury. This beneficial effect was accompanied by the reduction in hippocampal pro-inflammatory and pro-apoptotic indicators, and enhanced BDNF and GABA-ARα2S gene expression, previously observed in untreated burns. The hippocampal relative gene expression of melatonin receptors and NPY positively responded to the applied protocols, in the same manner as µ and δ opioid receptors, while the opposite response was observed for κ receptors. The results of this study provide some confirmations that adjuvant strategies, such as HBO and antioxidant supplementation, may be simultaneously applied in the treatment of the anxiety-like behavior that coincides with thermal skin injury. |
| first_indexed | 2024-04-25T00:27:31Z |
| format | Article |
| id | doaj.art-3523f6bcbce0474c931aa1182c5c9f5a |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:27:31Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3523f6bcbce0474c931aa1182c5c9f5a2024-03-12T16:47:19ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01255303310.3390/ijms25053033Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and <i>Filipendula ulmaria</i> Extract in RatsBojana Krstic0Dragica Selakovic1Nemanja Jovicic2Milos Krstic3Jelena S. Katanic Stankovic4Sara Rosic5Dragan Milovanovic6Gvozden Rosic7Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, SerbiaDepartment of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, SerbiaDepartment of Histology and Embryology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, SerbiaDepartment of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, SerbiaDepartment of Science, Institute for Information Technologies Kragujevac, University of Kragujevac, 34000 Kragujevac, SerbiaDepartment of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, SerbiaClinical Pharmacology Department, Clinical Centre Kragujevac, 34000 Kragujevac, SerbiaDepartment of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, SerbiaThe aim of this study was to evaluate the alterations of the hippocampal function that may be related to anxiogenic response to thermal skin injury, including the morpho-functional alterations, and the effects of hyperbaric oxygen (HBO) and <i>Filipendula ulmaria</i> (FU) extract in the treatment of anxiety-like behavior that coincides with thermal skin injury. A rat thermal skin injury experimental model was performed on 2-month-old male <i>Wistar albino</i> rats. The evaluated therapeutic protocols included HBO and/or antioxidant supplementation. HBO was applied for 7 days in the hyperbaric chamber (100% O<sub>2</sub>, 2.5 ATA, 60 min). Oral administration of FU extract (final concentration of 100 mg/kg b.w.) to achieve antioxidant supplementation was also applied for 7 days. Anxiety level was estimated in the open field and elevated plus-maze test, which was followed by anesthesia, sacrifice, and collection of hippocampal tissue samples. HBO treatment and FU supplementation significantly abolished anxiogenic response to thermal skin injury. This beneficial effect was accompanied by the reduction in hippocampal pro-inflammatory and pro-apoptotic indicators, and enhanced BDNF and GABA-ARα2S gene expression, previously observed in untreated burns. The hippocampal relative gene expression of melatonin receptors and NPY positively responded to the applied protocols, in the same manner as µ and δ opioid receptors, while the opposite response was observed for κ receptors. The results of this study provide some confirmations that adjuvant strategies, such as HBO and antioxidant supplementation, may be simultaneously applied in the treatment of the anxiety-like behavior that coincides with thermal skin injury.https://www.mdpi.com/1422-0067/25/5/3033hippocampusthermal skin injuryanxietyhyperbaric oxygen therapy<i>Filipendula ulmaria</i> extractrats |
| spellingShingle | Bojana Krstic Dragica Selakovic Nemanja Jovicic Milos Krstic Jelena S. Katanic Stankovic Sara Rosic Dragan Milovanovic Gvozden Rosic Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and <i>Filipendula ulmaria</i> Extract in Rats International Journal of Molecular Sciences hippocampus thermal skin injury anxiety hyperbaric oxygen therapy <i>Filipendula ulmaria</i> extract rats |
| title | Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and <i>Filipendula ulmaria</i> Extract in Rats |
| title_full | Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and <i>Filipendula ulmaria</i> Extract in Rats |
| title_fullStr | Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and <i>Filipendula ulmaria</i> Extract in Rats |
| title_full_unstemmed | Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and <i>Filipendula ulmaria</i> Extract in Rats |
| title_short | Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and <i>Filipendula ulmaria</i> Extract in Rats |
| title_sort | complex hippocampal response to thermal skin injury and protocols with hyperbaric oxygen therapy and i filipendula ulmaria i extract in rats |
| topic | hippocampus thermal skin injury anxiety hyperbaric oxygen therapy <i>Filipendula ulmaria</i> extract rats |
| url | https://www.mdpi.com/1422-0067/25/5/3033 |
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