Enhanced Growth of Bacterial Cells in a Smart 3D Printed Bioreactor
In the last decade, there has been a notable advancement in diverse bioreactor types catering to various applications. However, conventional bioreactors often exhibit bulkiness and high costs, making them less accessible to many researchers and laboratory facilities. In light of these challenges, th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/10/1829 |
| _version_ | 1827720372134346752 |
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| author | Eleftheria Maria Pechlivani Sotirios Pemas Alexandros Kanlis Paraskevi Pechlivani Spyros Petrakis Athanasios Papadimitriou Dimitrios Tzovaras Konstantinos E. Hatzistergos |
| author_facet | Eleftheria Maria Pechlivani Sotirios Pemas Alexandros Kanlis Paraskevi Pechlivani Spyros Petrakis Athanasios Papadimitriou Dimitrios Tzovaras Konstantinos E. Hatzistergos |
| author_sort | Eleftheria Maria Pechlivani |
| collection | DOAJ |
| description | In the last decade, there has been a notable advancement in diverse bioreactor types catering to various applications. However, conventional bioreactors often exhibit bulkiness and high costs, making them less accessible to many researchers and laboratory facilities. In light of these challenges, this article aims to introduce and evaluate the development of a do-it-yourself (DIY) 3D printed smart bioreactor, offering a cost-effective and user-friendly solution for the proliferation of various bioentities, including bacteria and human organoids, among others. The customized bioreactor was fabricated under an ergonomic design and assembled with 3D printed mechanical parts combined with electronic components, under 3D printed housing. The 3D printed parts were designed using SOLIDWORKS<sup>®</sup> CAD Software (2022 SP2.0 Professional version) and fabricated via the fused filament fabrication (FFF) technique. All parts were 3D printed with acrylonitrile butadiene styrene (ABS) in order for the bioreactor to be used under sterile conditions. The printed low-cost bioreactor integrates Internet-of-things (IoT) functionalities, since it provides the operator with the ability to change its operational parameters (sampling frequency, rotor speed, and duty cycle) remotely, via a user-friendly developed mobile application and to save the user history locally on the device. Using this bioreactor, which is adjusted to a standard commercial 12-well plate, proof of concept of a successful operation of the bioreactor during a 2-day culture of <i>Escherichia coli</i> bacteria (Mach1 strain) is presented. This study paves the way for more in-depth investigation of bacterial and various biological-entity growth cultures, utilizing 3D printing technology to create customized low-cost bioreactors. |
| first_indexed | 2024-03-10T21:02:24Z |
| format | Article |
| id | doaj.art-352752e913894d0ea9d73c1133c564ec |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T21:02:24Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-352752e913894d0ea9d73c1133c564ec2023-11-19T17:23:25ZengMDPI AGMicromachines2072-666X2023-09-011410182910.3390/mi14101829Enhanced Growth of Bacterial Cells in a Smart 3D Printed BioreactorEleftheria Maria Pechlivani0Sotirios Pemas1Alexandros Kanlis2Paraskevi Pechlivani3Spyros Petrakis4Athanasios Papadimitriou5Dimitrios Tzovaras6Konstantinos E. Hatzistergos7Centre for Research and Technology Hellas, Information Technologies Institute, 6th km Charilaou-Thermi Road, 57001 Thessaloniki, GreeceCentre for Research and Technology Hellas, Information Technologies Institute, 6th km Charilaou-Thermi Road, 57001 Thessaloniki, GreeceCentre for Research and Technology Hellas, Information Technologies Institute, 6th km Charilaou-Thermi Road, 57001 Thessaloniki, GreeceCentre for Research and Technology Hellas, Institute of Applied Biosciences, 6th km Charilaou-Thermi Road, 57001 Thessaloniki, GreeceCentre for Research and Technology Hellas, Institute of Applied Biosciences, 6th km Charilaou-Thermi Road, 57001 Thessaloniki, GreeceCentre for Research and Technology Hellas, Information Technologies Institute, 6th km Charilaou-Thermi Road, 57001 Thessaloniki, GreeceCentre for Research and Technology Hellas, Information Technologies Institute, 6th km Charilaou-Thermi Road, 57001 Thessaloniki, GreeceDepartment of Genetics, Development and Molecular Biology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceIn the last decade, there has been a notable advancement in diverse bioreactor types catering to various applications. However, conventional bioreactors often exhibit bulkiness and high costs, making them less accessible to many researchers and laboratory facilities. In light of these challenges, this article aims to introduce and evaluate the development of a do-it-yourself (DIY) 3D printed smart bioreactor, offering a cost-effective and user-friendly solution for the proliferation of various bioentities, including bacteria and human organoids, among others. The customized bioreactor was fabricated under an ergonomic design and assembled with 3D printed mechanical parts combined with electronic components, under 3D printed housing. The 3D printed parts were designed using SOLIDWORKS<sup>®</sup> CAD Software (2022 SP2.0 Professional version) and fabricated via the fused filament fabrication (FFF) technique. All parts were 3D printed with acrylonitrile butadiene styrene (ABS) in order for the bioreactor to be used under sterile conditions. The printed low-cost bioreactor integrates Internet-of-things (IoT) functionalities, since it provides the operator with the ability to change its operational parameters (sampling frequency, rotor speed, and duty cycle) remotely, via a user-friendly developed mobile application and to save the user history locally on the device. Using this bioreactor, which is adjusted to a standard commercial 12-well plate, proof of concept of a successful operation of the bioreactor during a 2-day culture of <i>Escherichia coli</i> bacteria (Mach1 strain) is presented. This study paves the way for more in-depth investigation of bacterial and various biological-entity growth cultures, utilizing 3D printing technology to create customized low-cost bioreactors.https://www.mdpi.com/2072-666X/14/10/1829additive manufacturing3D printingbioreactorIoT technologiesmobile user interfacebacterial growth |
| spellingShingle | Eleftheria Maria Pechlivani Sotirios Pemas Alexandros Kanlis Paraskevi Pechlivani Spyros Petrakis Athanasios Papadimitriou Dimitrios Tzovaras Konstantinos E. Hatzistergos Enhanced Growth of Bacterial Cells in a Smart 3D Printed Bioreactor Micromachines additive manufacturing 3D printing bioreactor IoT technologies mobile user interface bacterial growth |
| title | Enhanced Growth of Bacterial Cells in a Smart 3D Printed Bioreactor |
| title_full | Enhanced Growth of Bacterial Cells in a Smart 3D Printed Bioreactor |
| title_fullStr | Enhanced Growth of Bacterial Cells in a Smart 3D Printed Bioreactor |
| title_full_unstemmed | Enhanced Growth of Bacterial Cells in a Smart 3D Printed Bioreactor |
| title_short | Enhanced Growth of Bacterial Cells in a Smart 3D Printed Bioreactor |
| title_sort | enhanced growth of bacterial cells in a smart 3d printed bioreactor |
| topic | additive manufacturing 3D printing bioreactor IoT technologies mobile user interface bacterial growth |
| url | https://www.mdpi.com/2072-666X/14/10/1829 |
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