Occurrence of Metallo-β-lactamase Genes among Acinetobacter baumannii Isolated from Different Clinical Samples
Acinetobacter baumannii became a serious endemic and widespread pathogen responsible for causing nosocomial infections due to restricted treatment options. This study was conducted to evaluate the role of both antibiotic resistance and genetic pattern in infection caused by MBLs resistance A. baum...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2019-06-01
|
| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/occurrence-of-metallo-%ce%b2-lactamase-genes-among-acinetobacter-baumannii-isolated-from-different-clinical-samples/ |
| _version_ | 1830440833780809728 |
|---|---|
| author | Dunya Jawad Ridha Munim Radwan Ali Kifah Ahmed Jassim |
| author_facet | Dunya Jawad Ridha Munim Radwan Ali Kifah Ahmed Jassim |
| author_sort | Dunya Jawad Ridha |
| collection | DOAJ |
| description | Acinetobacter baumannii became a serious endemic and widespread pathogen responsible for causing
nosocomial infections due to restricted treatment options. This study was conducted to evaluate the role
of both antibiotic resistance and genetic pattern in infection caused by MBLs resistance A. baumannii
isolated in Baghdad hospitals. Finally, developing selective media in order to detect multidrug resistance
A. baumannii isolates according to colony shape appearance changing. Collected 124 isolates from
various clinical and environmental sources. Biofilm Formation was detected, Antibiotic sensitive for
21 antibiotic discs were determined, MDR index calculated, PCR was performed to investigate MBLs
genes bla (IMP2, KPC, DIM, SPM, VIM, GIM, AIM, BIC, SIM, NDM, Pre-NDM-A, GES, Imp-1, and VIM).
Increasing resistant prevalence of A. baumannii appear significantly in higher rates to seven antibiotics
group and MDR index ranged between 0.29 to 1.0 from 6-21 antibiotics. It was clear in Biofilm formation
evaluated 75.8% strong biofilm after 72 hours, indicated that there is a significant negative correlation
between biofilm formation capacity and the inherent ability of bacteria to show multidrug resist (P<
0.001). Our findings indicated that non-MDR isolates tended to form more robust biofilm formation.
Metallo β-Lactamase detection phenotype showed 113/124 isolates able to produced ESBL, a result
confirmed by PCR assay to detect the resistance of MBL-genes, appeared that a high percentage of
blaIMP 79% which left clinicians with limited treatment options. An increasing number of hospital MDR
A. baumannii has been reported. Furthermore, the study will focus on the evolution and the emergence
of multidrug resistance A. baumannii in Iraq. The common MBL gene is the blaIMP. |
| first_indexed | 2024-12-21T05:11:38Z |
| format | Article |
| id | doaj.art-3528ee07160d496680bf087cbe715c19 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-21T05:11:38Z |
| publishDate | 2019-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-3528ee07160d496680bf087cbe715c192022-12-21T19:15:02ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2019-06-011321111111910.22207/JPAM.13.2.50Occurrence of Metallo-β-lactamase Genes among Acinetobacter baumannii Isolated from Different Clinical SamplesDunya Jawad Ridha0Munim Radwan Ali1Kifah Ahmed Jassim2National Center for Educational Laboratories, Medical City, Ministry of Health Baghdad, Iraq.Al-Musatnsirihyah University, College of Science, Department of Biology, Baghdad, Iraq.Baghdad Health Department, Rusafa, Ministry of Health, Baghdad, Iraq.Acinetobacter baumannii became a serious endemic and widespread pathogen responsible for causing nosocomial infections due to restricted treatment options. This study was conducted to evaluate the role of both antibiotic resistance and genetic pattern in infection caused by MBLs resistance A. baumannii isolated in Baghdad hospitals. Finally, developing selective media in order to detect multidrug resistance A. baumannii isolates according to colony shape appearance changing. Collected 124 isolates from various clinical and environmental sources. Biofilm Formation was detected, Antibiotic sensitive for 21 antibiotic discs were determined, MDR index calculated, PCR was performed to investigate MBLs genes bla (IMP2, KPC, DIM, SPM, VIM, GIM, AIM, BIC, SIM, NDM, Pre-NDM-A, GES, Imp-1, and VIM). Increasing resistant prevalence of A. baumannii appear significantly in higher rates to seven antibiotics group and MDR index ranged between 0.29 to 1.0 from 6-21 antibiotics. It was clear in Biofilm formation evaluated 75.8% strong biofilm after 72 hours, indicated that there is a significant negative correlation between biofilm formation capacity and the inherent ability of bacteria to show multidrug resist (P< 0.001). Our findings indicated that non-MDR isolates tended to form more robust biofilm formation. Metallo β-Lactamase detection phenotype showed 113/124 isolates able to produced ESBL, a result confirmed by PCR assay to detect the resistance of MBL-genes, appeared that a high percentage of blaIMP 79% which left clinicians with limited treatment options. An increasing number of hospital MDR A. baumannii has been reported. Furthermore, the study will focus on the evolution and the emergence of multidrug resistance A. baumannii in Iraq. The common MBL gene is the blaIMP.https://microbiologyjournal.org/occurrence-of-metallo-%ce%b2-lactamase-genes-among-acinetobacter-baumannii-isolated-from-different-clinical-samples/acinetobacter baumanniimetallo-β-lactamase genesbiofilmmultidrug resistance |
| spellingShingle | Dunya Jawad Ridha Munim Radwan Ali Kifah Ahmed Jassim Occurrence of Metallo-β-lactamase Genes among Acinetobacter baumannii Isolated from Different Clinical Samples Journal of Pure and Applied Microbiology acinetobacter baumannii metallo-β-lactamase genes biofilm multidrug resistance |
| title | Occurrence of Metallo-β-lactamase Genes among Acinetobacter baumannii Isolated from Different Clinical Samples |
| title_full | Occurrence of Metallo-β-lactamase Genes among Acinetobacter baumannii Isolated from Different Clinical Samples |
| title_fullStr | Occurrence of Metallo-β-lactamase Genes among Acinetobacter baumannii Isolated from Different Clinical Samples |
| title_full_unstemmed | Occurrence of Metallo-β-lactamase Genes among Acinetobacter baumannii Isolated from Different Clinical Samples |
| title_short | Occurrence of Metallo-β-lactamase Genes among Acinetobacter baumannii Isolated from Different Clinical Samples |
| title_sort | occurrence of metallo β lactamase genes among acinetobacter baumannii isolated from different clinical samples |
| topic | acinetobacter baumannii metallo-β-lactamase genes biofilm multidrug resistance |
| url | https://microbiologyjournal.org/occurrence-of-metallo-%ce%b2-lactamase-genes-among-acinetobacter-baumannii-isolated-from-different-clinical-samples/ |
| work_keys_str_mv | AT dunyajawadridha occurrenceofmetalloblactamasegenesamongacinetobacterbaumanniiisolatedfromdifferentclinicalsamples AT munimradwanali occurrenceofmetalloblactamasegenesamongacinetobacterbaumanniiisolatedfromdifferentclinicalsamples AT kifahahmedjassim occurrenceofmetalloblactamasegenesamongacinetobacterbaumanniiisolatedfromdifferentclinicalsamples |