Determination of Peak Purity in HPLC by Coupling Coulometric Array Detection and Two-Dimensional Correlation Analysis
This work aims to evaluate the purity of chromatographic peaks by a two-dimensional correlation (2D-corr) analysis. Such an analysis leads to two contour plots: synchronous and asynchronous. The synchronous contour plot provides information on the number of peaks present in the chromatogram. The asy...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/5/1794 |
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| author | Julio Enrique Oney-Montalvo Ksenia Morozova Manuel Octavio Ramírez-Sucre Matteo Scampicchio Ingrid Mayanin Rodríguez-Buenfil |
| author_facet | Julio Enrique Oney-Montalvo Ksenia Morozova Manuel Octavio Ramírez-Sucre Matteo Scampicchio Ingrid Mayanin Rodríguez-Buenfil |
| author_sort | Julio Enrique Oney-Montalvo |
| collection | DOAJ |
| description | This work aims to evaluate the purity of chromatographic peaks by a two-dimensional correlation (2D-corr) analysis. Such an analysis leads to two contour plots: synchronous and asynchronous. The synchronous contour plot provides information on the number of peaks present in the chromatogram. The asynchronous contour plot reveals the presence of overlapping species on each peak. The utility of 2D-corr analysis was demonstrated by the chromatographic analysis of <i>Capsicum</i> chili extracts obtained by HPLC coupled with a coulometric array of sixteen detectors. Thanks to 16 electrochemical sensors, each poised at increasing potentials, the resulting 2D-corr analysis revealed the presence of at least three species on the peak located at a retention time of 0.93 min. Mass spectrometry (MS) analysis was used to analyze the coeluting species, which were identified as: quinic acid (3.593 min), ascorbic acid (3.943 min), and phenylalanine (4.229 min). Overall, this work supports the use of 2D-corr analysis to reveal the presence of overlapping compounds and, thus, verify the signal purity of chromatographic peaks. |
| first_indexed | 2024-03-09T20:21:08Z |
| format | Article |
| id | doaj.art-352a30e19e404420b5770f3d6a9d17c1 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T20:21:08Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-352a30e19e404420b5770f3d6a9d17c12023-11-23T23:46:17ZengMDPI AGSensors1424-82202022-02-01225179410.3390/s22051794Determination of Peak Purity in HPLC by Coupling Coulometric Array Detection and Two-Dimensional Correlation AnalysisJulio Enrique Oney-Montalvo0Ksenia Morozova1Manuel Octavio Ramírez-Sucre2Matteo Scampicchio3Ingrid Mayanin Rodríguez-Buenfil4Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Sede Sureste, Tablaje Catastral No. 31264, km 5.5 Carretera Sierra Papacal-Chuburna Puerto, Interior del Parque Científico y Tecnológico Yucatán, Mérida 97302, Yucatán, MexicoFaculty of Science and Technology, Free University of Bozen-Bolzano, Piazza Università 5, 39100 Bolzano, ItalyCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Sede Sureste, Tablaje Catastral No. 31264, km 5.5 Carretera Sierra Papacal-Chuburna Puerto, Interior del Parque Científico y Tecnológico Yucatán, Mérida 97302, Yucatán, MexicoFaculty of Science and Technology, Free University of Bozen-Bolzano, Piazza Università 5, 39100 Bolzano, ItalyCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Sede Sureste, Tablaje Catastral No. 31264, km 5.5 Carretera Sierra Papacal-Chuburna Puerto, Interior del Parque Científico y Tecnológico Yucatán, Mérida 97302, Yucatán, MexicoThis work aims to evaluate the purity of chromatographic peaks by a two-dimensional correlation (2D-corr) analysis. Such an analysis leads to two contour plots: synchronous and asynchronous. The synchronous contour plot provides information on the number of peaks present in the chromatogram. The asynchronous contour plot reveals the presence of overlapping species on each peak. The utility of 2D-corr analysis was demonstrated by the chromatographic analysis of <i>Capsicum</i> chili extracts obtained by HPLC coupled with a coulometric array of sixteen detectors. Thanks to 16 electrochemical sensors, each poised at increasing potentials, the resulting 2D-corr analysis revealed the presence of at least three species on the peak located at a retention time of 0.93 min. Mass spectrometry (MS) analysis was used to analyze the coeluting species, which were identified as: quinic acid (3.593 min), ascorbic acid (3.943 min), and phenylalanine (4.229 min). Overall, this work supports the use of 2D-corr analysis to reveal the presence of overlapping compounds and, thus, verify the signal purity of chromatographic peaks.https://www.mdpi.com/1424-8220/22/5/1794coulometric sensorelectrochemical sensor2D correlation analysisfood sensorsliquid chromatographymass spectrometry |
| spellingShingle | Julio Enrique Oney-Montalvo Ksenia Morozova Manuel Octavio Ramírez-Sucre Matteo Scampicchio Ingrid Mayanin Rodríguez-Buenfil Determination of Peak Purity in HPLC by Coupling Coulometric Array Detection and Two-Dimensional Correlation Analysis Sensors coulometric sensor electrochemical sensor 2D correlation analysis food sensors liquid chromatography mass spectrometry |
| title | Determination of Peak Purity in HPLC by Coupling Coulometric Array Detection and Two-Dimensional Correlation Analysis |
| title_full | Determination of Peak Purity in HPLC by Coupling Coulometric Array Detection and Two-Dimensional Correlation Analysis |
| title_fullStr | Determination of Peak Purity in HPLC by Coupling Coulometric Array Detection and Two-Dimensional Correlation Analysis |
| title_full_unstemmed | Determination of Peak Purity in HPLC by Coupling Coulometric Array Detection and Two-Dimensional Correlation Analysis |
| title_short | Determination of Peak Purity in HPLC by Coupling Coulometric Array Detection and Two-Dimensional Correlation Analysis |
| title_sort | determination of peak purity in hplc by coupling coulometric array detection and two dimensional correlation analysis |
| topic | coulometric sensor electrochemical sensor 2D correlation analysis food sensors liquid chromatography mass spectrometry |
| url | https://www.mdpi.com/1424-8220/22/5/1794 |
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