A Polyphasic Approach Reveals Novel Genotypes and Updates the Genetic Structure of the Banana Fusarium Wilt Pathogen
<i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc) is a soil-borne fungus that causes Fusarium wilt, a destructive plant disease that has resulted in devastating economic losses to banana production worldwide. The fungus has a complex evolutionary history and taxonomic rep...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/2/269 |
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| author | Diane Mostert Emmanuel Wicker Mignon M. de Jager Saif M. Al Kaabi Wayne T. O’Neill Suzy Perry Chunyu Li Yi Ganyun Kenneth G. Pegg Lizel Mostert Altus Viljoen |
| author_facet | Diane Mostert Emmanuel Wicker Mignon M. de Jager Saif M. Al Kaabi Wayne T. O’Neill Suzy Perry Chunyu Li Yi Ganyun Kenneth G. Pegg Lizel Mostert Altus Viljoen |
| author_sort | Diane Mostert |
| collection | DOAJ |
| description | <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc) is a soil-borne fungus that causes Fusarium wilt, a destructive plant disease that has resulted in devastating economic losses to banana production worldwide. The fungus has a complex evolutionary history and taxonomic repute and consists of three pathogenic races and at least 24 vegetative compatibility groups (VCGs). Surveys conducted in Asia, Africa, the Sultanate of Oman and Mauritius encountered isolates of <i>F. oxysporum</i> pathogenic to banana that were not compatible to any of the known Foc VCGs. Genetic relatedness between the undescribed and known Foc VCGs were determined using a multi-gene phylogeny and diversity array technology (DArT) sequencing. The presence of putative effector genes, the <i>secreted in xylem</i> (<i>SIX</i>) genes, were also determined. Fourteen novel Foc VCGs and 17 single-member VCGs were identified. The multi-gene tree was congruent with the DArT-seq phylogeny and divided the novel VCGs into three clades. Clustering analysis of the DArT-seq data supported the separation of Foc isolates into eight distinct clusters, with the suite of <i>SIX</i> genes mostly conserved within these clusters. Results from this study indicates that Foc is more diverse than hitherto assumed. |
| first_indexed | 2024-03-09T21:23:29Z |
| format | Article |
| id | doaj.art-3530933a2b47425aa9fa6eb932ac9b94 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:23:29Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3530933a2b47425aa9fa6eb932ac9b942023-11-23T21:13:27ZengMDPI AGMicroorganisms2076-26072022-01-0110226910.3390/microorganisms10020269A Polyphasic Approach Reveals Novel Genotypes and Updates the Genetic Structure of the Banana Fusarium Wilt PathogenDiane Mostert0Emmanuel Wicker1Mignon M. de Jager2Saif M. Al Kaabi3Wayne T. O’Neill4Suzy Perry5Chunyu Li6Yi Ganyun7Kenneth G. Pegg8Lizel Mostert9Altus Viljoen10Department of Plant Pathology, Stellenbosch University, Stellenbosch 7600, South AfricaCIRAD, UMR PHIM, 34398 Montpellier, FranceDepartment of Plant Pathology, Stellenbosch University, Stellenbosch 7600, South AfricaDepartment of Plant Pathology, Stellenbosch University, Stellenbosch 7600, South AfricaEcosciences Precinct, Department of Agriculture and Fisheries, Brisbane, QLD 4102, AustraliaEcosciences Precinct, Department of Agriculture and Fisheries, Brisbane, QLD 4102, AustraliaInstitution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaInstitution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaEcosciences Precinct, Department of Agriculture and Fisheries, Brisbane, QLD 4102, AustraliaDepartment of Plant Pathology, Stellenbosch University, Stellenbosch 7600, South AfricaDepartment of Plant Pathology, Stellenbosch University, Stellenbosch 7600, South Africa<i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc) is a soil-borne fungus that causes Fusarium wilt, a destructive plant disease that has resulted in devastating economic losses to banana production worldwide. The fungus has a complex evolutionary history and taxonomic repute and consists of three pathogenic races and at least 24 vegetative compatibility groups (VCGs). Surveys conducted in Asia, Africa, the Sultanate of Oman and Mauritius encountered isolates of <i>F. oxysporum</i> pathogenic to banana that were not compatible to any of the known Foc VCGs. Genetic relatedness between the undescribed and known Foc VCGs were determined using a multi-gene phylogeny and diversity array technology (DArT) sequencing. The presence of putative effector genes, the <i>secreted in xylem</i> (<i>SIX</i>) genes, were also determined. Fourteen novel Foc VCGs and 17 single-member VCGs were identified. The multi-gene tree was congruent with the DArT-seq phylogeny and divided the novel VCGs into three clades. Clustering analysis of the DArT-seq data supported the separation of Foc isolates into eight distinct clusters, with the suite of <i>SIX</i> genes mostly conserved within these clusters. Results from this study indicates that Foc is more diverse than hitherto assumed.https://www.mdpi.com/2076-2607/10/2/269banana Fusarium wiltdiversity array technologyphylogeny<i>secreted in xylem</i> genesvegetative compatibility |
| spellingShingle | Diane Mostert Emmanuel Wicker Mignon M. de Jager Saif M. Al Kaabi Wayne T. O’Neill Suzy Perry Chunyu Li Yi Ganyun Kenneth G. Pegg Lizel Mostert Altus Viljoen A Polyphasic Approach Reveals Novel Genotypes and Updates the Genetic Structure of the Banana Fusarium Wilt Pathogen Microorganisms banana Fusarium wilt diversity array technology phylogeny <i>secreted in xylem</i> genes vegetative compatibility |
| title | A Polyphasic Approach Reveals Novel Genotypes and Updates the Genetic Structure of the Banana Fusarium Wilt Pathogen |
| title_full | A Polyphasic Approach Reveals Novel Genotypes and Updates the Genetic Structure of the Banana Fusarium Wilt Pathogen |
| title_fullStr | A Polyphasic Approach Reveals Novel Genotypes and Updates the Genetic Structure of the Banana Fusarium Wilt Pathogen |
| title_full_unstemmed | A Polyphasic Approach Reveals Novel Genotypes and Updates the Genetic Structure of the Banana Fusarium Wilt Pathogen |
| title_short | A Polyphasic Approach Reveals Novel Genotypes and Updates the Genetic Structure of the Banana Fusarium Wilt Pathogen |
| title_sort | polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen |
| topic | banana Fusarium wilt diversity array technology phylogeny <i>secreted in xylem</i> genes vegetative compatibility |
| url | https://www.mdpi.com/2076-2607/10/2/269 |
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