LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics
Optogenetic techniques offer a high spatiotemporal resolution to manipulate cellular activity. For instance, Channelrhodopsin-2 with global light illumination is the most widely used to control neuronal activity at the cellular level. However, the cellular scale is much larger than the diffraction l...
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| Format: | Article |
| Language: | English |
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The Biophysical Society of Japan
2023-06-01
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| Series: | Biophysics and Physicobiology |
| Subjects: | |
| Online Access: | https://doi.org/10.2142/biophysico.bppb-v20.0027 |
| _version_ | 1797797050767638528 |
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| author | Yutaro Nagasawa Hiromi H. Ueda Haruka Kawabata Hideji Murakoshi |
| author_facet | Yutaro Nagasawa Hiromi H. Ueda Haruka Kawabata Hideji Murakoshi |
| author_sort | Yutaro Nagasawa |
| collection | DOAJ |
| description | Optogenetic techniques offer a high spatiotemporal resolution to manipulate cellular activity. For instance, Channelrhodopsin-2 with global light illumination is the most widely used to control neuronal activity at the cellular level. However, the cellular scale is much larger than the diffraction limit of light (<1 μm) and does not fully exploit the features of the “high spatial resolution” of optogenetics. For instance, until recently, there were no optogenetic methods to induce synaptic plasticity at the level of single synapses. To address this, we developed an optogenetic tool named photoactivatable CaMKII (paCaMKII) by fusing a light-sensitive domain (LOV2) to CaMKIIα, which is a protein abundantly expressed in neurons of the cerebrum and hippocampus and essential for synaptic plasticity. Combining photoactivatable CaMKII with two-photon excitation, we successfully activated it in single spines, inducing synaptic plasticity (long-term potentiation) in hippocampal neurons. We refer to this method as “Local Optogenetics”, which involves the local activation of molecules and measurement of cellular responses. In this review, we will discuss the characteristics of LOV2, the recent development of its derivatives, and the development and application of paCaMKII. |
| first_indexed | 2024-03-13T03:42:19Z |
| format | Article |
| id | doaj.art-3535e8aa1fb143269c113529a3988714 |
| institution | Directory Open Access Journal |
| issn | 2189-4779 |
| language | English |
| last_indexed | 2024-03-13T03:42:19Z |
| publishDate | 2023-06-01 |
| publisher | The Biophysical Society of Japan |
| record_format | Article |
| series | Biophysics and Physicobiology |
| spelling | doaj.art-3535e8aa1fb143269c113529a39887142023-06-23T05:18:13ZengThe Biophysical Society of JapanBiophysics and Physicobiology2189-47792023-06-012010.2142/biophysico.bppb-v20.0027LOV2-based photoactivatable CaMKII and its application to single synapses: Local OptogeneticsYutaro Nagasawa0Hiromi H. Ueda1Haruka Kawabata2Hideji Murakoshi3Supportive Center for Brain Research, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, JapanSupportive Center for Brain Research, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, JapanSupportive Center for Brain Research, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, JapanSupportive Center for Brain Research, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, JapanOptogenetic techniques offer a high spatiotemporal resolution to manipulate cellular activity. For instance, Channelrhodopsin-2 with global light illumination is the most widely used to control neuronal activity at the cellular level. However, the cellular scale is much larger than the diffraction limit of light (<1 μm) and does not fully exploit the features of the “high spatial resolution” of optogenetics. For instance, until recently, there were no optogenetic methods to induce synaptic plasticity at the level of single synapses. To address this, we developed an optogenetic tool named photoactivatable CaMKII (paCaMKII) by fusing a light-sensitive domain (LOV2) to CaMKIIα, which is a protein abundantly expressed in neurons of the cerebrum and hippocampus and essential for synaptic plasticity. Combining photoactivatable CaMKII with two-photon excitation, we successfully activated it in single spines, inducing synaptic plasticity (long-term potentiation) in hippocampal neurons. We refer to this method as “Local Optogenetics”, which involves the local activation of molecules and measurement of cellular responses. In this review, we will discuss the characteristics of LOV2, the recent development of its derivatives, and the development and application of paCaMKII.https://doi.org/10.2142/biophysico.bppb-v20.0027lov2photoactivatable camkii (pacamkii)2-photon excitationdendritic spineexcitatory neuron |
| spellingShingle | Yutaro Nagasawa Hiromi H. Ueda Haruka Kawabata Hideji Murakoshi LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics Biophysics and Physicobiology lov2 photoactivatable camkii (pacamkii) 2-photon excitation dendritic spine excitatory neuron |
| title | LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics |
| title_full | LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics |
| title_fullStr | LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics |
| title_full_unstemmed | LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics |
| title_short | LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics |
| title_sort | lov2 based photoactivatable camkii and its application to single synapses local optogenetics |
| topic | lov2 photoactivatable camkii (pacamkii) 2-photon excitation dendritic spine excitatory neuron |
| url | https://doi.org/10.2142/biophysico.bppb-v20.0027 |
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