Isolation, Characterization, and Transcriptome Analysis of an ISKNV-Like Virus from Largemouth Bass
Largemouth bass (<i>Micropterus salmoides</i>) is an important commercial fish farmed in China. Challenges related to diseases caused by pathogens, such as iridovirus, have become increasingly serious. In 2017, we detected iridovirus-infected diseased largemouth bass in Zunyi, Guizhou Pr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/2/398 |
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| author | Zhuqing Xu Jiaming Liao Dongzhuo Zhang Shaoli Liu Luhao Zhang Shaozhu Kang Linting Xu Hong Chen Wenquan Peng Sheng Zhou Qiwei Qin Jingguang Wei |
| author_facet | Zhuqing Xu Jiaming Liao Dongzhuo Zhang Shaoli Liu Luhao Zhang Shaozhu Kang Linting Xu Hong Chen Wenquan Peng Sheng Zhou Qiwei Qin Jingguang Wei |
| author_sort | Zhuqing Xu |
| collection | DOAJ |
| description | Largemouth bass (<i>Micropterus salmoides</i>) is an important commercial fish farmed in China. Challenges related to diseases caused by pathogens, such as iridovirus, have become increasingly serious. In 2017, we detected iridovirus-infected diseased largemouth bass in Zunyi, Guizhou Province. The isolated virus was identified as an infectious spleen and kidney necrosis virus (ISKNV)-like virus (ISKNV-ZY). ISKNV-ZY induces a cytopathic effect after infecting mandarin fish brain (MFB) cells. Abundant hexagonal virus particles were observed in the cytoplasm of ISKNV-ZY-infected MFB cells, using electron microscopy. The whole genome of ISKNV-ZY contained 112,248 bp and 122 open reading frames. Phylogenetic tree analysis showed that ISKNV-ZY was most closely related to BCIV, indicating that it is an ISKNV-like megalocytivirus. ISKNV-ZY-infected largemouth bass started to die on day six and reached a death peak on days 7–8. Cumulative mortality reached 100% on day 10. Using RNA sequencing-based transcriptome analysis after ISKNV-ZY infection, 6254 differentially expressed unigenes (DEGs) were identified, of which 3518 were upregulated and 2673 downregulated. The DEGs were associated with endocytosis, thermogenesis, oxidative phosphorylation, the JAK-STAT signaling pathway, the MAPK signaling pathway, etc. These results contribute to understanding the molecular regulation mechanism of ISKNV infection and provide a basis for ISKNV prevention. |
| first_indexed | 2024-03-11T08:00:32Z |
| format | Article |
| id | doaj.art-3536ce7610e74147beac51a15c39ace2 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T08:00:32Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-3536ce7610e74147beac51a15c39ace22023-11-16T23:48:28ZengMDPI AGViruses1999-49152023-01-0115239810.3390/v15020398Isolation, Characterization, and Transcriptome Analysis of an ISKNV-Like Virus from Largemouth BassZhuqing Xu0Jiaming Liao1Dongzhuo Zhang2Shaoli Liu3Luhao Zhang4Shaozhu Kang5Linting Xu6Hong Chen7Wenquan Peng8Sheng Zhou9Qiwei Qin10Jingguang Wei11Guangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Winsun Biological Pharmaceutical Co., Ltd., Guangzhou 511356, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaLargemouth bass (<i>Micropterus salmoides</i>) is an important commercial fish farmed in China. Challenges related to diseases caused by pathogens, such as iridovirus, have become increasingly serious. In 2017, we detected iridovirus-infected diseased largemouth bass in Zunyi, Guizhou Province. The isolated virus was identified as an infectious spleen and kidney necrosis virus (ISKNV)-like virus (ISKNV-ZY). ISKNV-ZY induces a cytopathic effect after infecting mandarin fish brain (MFB) cells. Abundant hexagonal virus particles were observed in the cytoplasm of ISKNV-ZY-infected MFB cells, using electron microscopy. The whole genome of ISKNV-ZY contained 112,248 bp and 122 open reading frames. Phylogenetic tree analysis showed that ISKNV-ZY was most closely related to BCIV, indicating that it is an ISKNV-like megalocytivirus. ISKNV-ZY-infected largemouth bass started to die on day six and reached a death peak on days 7–8. Cumulative mortality reached 100% on day 10. Using RNA sequencing-based transcriptome analysis after ISKNV-ZY infection, 6254 differentially expressed unigenes (DEGs) were identified, of which 3518 were upregulated and 2673 downregulated. The DEGs were associated with endocytosis, thermogenesis, oxidative phosphorylation, the JAK-STAT signaling pathway, the MAPK signaling pathway, etc. These results contribute to understanding the molecular regulation mechanism of ISKNV infection and provide a basis for ISKNV prevention.https://www.mdpi.com/1999-4915/15/2/398<i>Micropterus salmoides</i>ISKNV-ZYisolationcharacterizationtranscriptome |
| spellingShingle | Zhuqing Xu Jiaming Liao Dongzhuo Zhang Shaoli Liu Luhao Zhang Shaozhu Kang Linting Xu Hong Chen Wenquan Peng Sheng Zhou Qiwei Qin Jingguang Wei Isolation, Characterization, and Transcriptome Analysis of an ISKNV-Like Virus from Largemouth Bass Viruses <i>Micropterus salmoides</i> ISKNV-ZY isolation characterization transcriptome |
| title | Isolation, Characterization, and Transcriptome Analysis of an ISKNV-Like Virus from Largemouth Bass |
| title_full | Isolation, Characterization, and Transcriptome Analysis of an ISKNV-Like Virus from Largemouth Bass |
| title_fullStr | Isolation, Characterization, and Transcriptome Analysis of an ISKNV-Like Virus from Largemouth Bass |
| title_full_unstemmed | Isolation, Characterization, and Transcriptome Analysis of an ISKNV-Like Virus from Largemouth Bass |
| title_short | Isolation, Characterization, and Transcriptome Analysis of an ISKNV-Like Virus from Largemouth Bass |
| title_sort | isolation characterization and transcriptome analysis of an isknv like virus from largemouth bass |
| topic | <i>Micropterus salmoides</i> ISKNV-ZY isolation characterization transcriptome |
| url | https://www.mdpi.com/1999-4915/15/2/398 |
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