Contribution to the Knowledge of Gastrointestinal Nematodes in Roe Deer (<i>Capreolus capreolus</i>) from the Province of León, Spain: An Epidemiological and Molecular Study
A study of gastrointestinal nematodes in roe deer was carried out in the regional hunting reserves of Riaño and Mampodre, Province of León, Spain, to provide information on their prevalence and intensity of infection in relation to the sampling areas, age of the animals, and body weight. Through a r...
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MDPI AG
2023-10-01
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| author | Sara González María Luisa del Rio Natividad Díez-Baños Angélica Martínez María del Rosario Hidalgo |
| author_facet | Sara González María Luisa del Rio Natividad Díez-Baños Angélica Martínez María del Rosario Hidalgo |
| author_sort | Sara González |
| collection | DOAJ |
| description | A study of gastrointestinal nematodes in roe deer was carried out in the regional hunting reserves of Riaño and Mampodre, Province of León, Spain, to provide information on their prevalence and intensity of infection in relation to the sampling areas, age of the animals, and body weight. Through a regulated necropsy of the animals, all of them harbored gastrointestinal nematodes in their digestive tract, with a mean intensity of parasitism of 638 ± 646.1 nematodes/infected animal. Eleven genera were found and 18 species of gastrointestinal nematodes were identified, three of them polymorphic: <i>Trichostrongylus axei</i>, <i>Trichostrongylus vitrinus</i>, <i>Trichostrongylus capricola</i>, <i>Trichostrongylus colubriformis</i>, <i>Haemonchus contortus</i>, <i>Spiculopteragia spiculoptera</i>/<i>Spiculopteragia mathevossiani</i>, <i>Ostertagia leptospicularis</i>/<i>Ostertagia kolchida</i>, <i>Ostertagia</i> (<i>Grosspiculopteragia</i>) <i>occidentalis</i>, <i>Teladorsagia circumcincta/Teladorsagia trifurcate</i>, <i>Marshallagia marshalli</i>, <i>Nematodirus europaeus</i>, <i>Cooperia oncophora</i>, <i>Capillaria bovis</i>, <i>Oesophagostomum venulosum,</i> and <i>Trichuris ovis</i>. All of them have already been cited in roe deer in Europe, but <i>Marshallagia marshalli</i>, <i>Capillaria bovis,</i> and <i>Ostertagia</i> (<i>Grosspiculopteragia</i>) <i>occidentalis</i> are reported for the first time in Spain in this host. The abomasum was the intestinal section, where the prevalence (98.9%) and mean intensity (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mover><mi mathvariant="normal">x</mi><mo>¯</mo></mover></mrow></semantics></math></inline-formula> = 370.7 ± 374.4 worms/roe deer; range 3–1762) were significantly higher, but no statistically significant differences were found when comparing the sampling areas and age of animals. The animals with lower body weight had a higher parasite load than those in better physical condition, finding, in this case, statistically significant differences (<i>p</i> = 0.0020). Seven genera and 14 species were identified. In the small intestine, 88% of the animals examined presented gastrointestinal nematodes, with an average intensity of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mover><mi mathvariant="normal">x</mi><mo>¯</mo></mover></mrow></semantics></math></inline-formula> = 131.7 ± 225.6 parasites/infected animal, ranging between 4–1254 worms. No statistically significant differences were found when the three parameters studied were compared. Four genera and seven species were identified. In the large intestine/cecum, 78.3% of the examined roe deer presented adult worms, with an average intensity of 6.3 ± 5.5 worms/infected animal; range 1–26 worms. Only statistically significant differences were observed when considering the mean intensity of parasitism and the sampling area (<i>p</i> = 0.0093). Two genera and two species were identified. Several of the species found in the study were studied molecularly, and with the sequences obtained compared with those deposited in GenBank, phylogenetic trees were prepared to determine their taxonomic status. Using coprological techniques, the existing correlation in the shedding of gastrointestinal nematode eggs in roe deer was investigated with that of semi-extensive sheep farms in the same study area to verify the existence of cross-transmission of these parasites between wild and domestic animals. The high values found in the studied parameters show that northern Spain is an area of high-intensity infection for roe deer. |
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| spelling | doaj.art-3537d04d4111459da1aa90156c80f6b52023-11-19T14:00:28ZengMDPI AGAnimals2076-26152023-10-011319311710.3390/ani13193117Contribution to the Knowledge of Gastrointestinal Nematodes in Roe Deer (<i>Capreolus capreolus</i>) from the Province of León, Spain: An Epidemiological and Molecular StudySara González0María Luisa del Rio1Natividad Díez-Baños2Angélica Martínez3María del Rosario Hidalgo4Department of Animal Health, Parasitology and Parasitic Diseases, Faculty of Veterinary Science, University of León, 24007 León, SpainDepartment of Animal Health, Section of Immunobiology, Faculty of Veterinary Science, University of León, 24007 León, SpainDepartment of Animal Health, Parasitology and Parasitic Diseases, Faculty of Veterinary Science, University of León, 24007 León, SpainDepartment of Biotechnology and Food Science, Faculty of Sciences, University of Burgos, 09001 Burgos, SpainDepartment of Animal Health, Parasitology and Parasitic Diseases, Faculty of Veterinary Science, University of León, 24007 León, SpainA study of gastrointestinal nematodes in roe deer was carried out in the regional hunting reserves of Riaño and Mampodre, Province of León, Spain, to provide information on their prevalence and intensity of infection in relation to the sampling areas, age of the animals, and body weight. Through a regulated necropsy of the animals, all of them harbored gastrointestinal nematodes in their digestive tract, with a mean intensity of parasitism of 638 ± 646.1 nematodes/infected animal. Eleven genera were found and 18 species of gastrointestinal nematodes were identified, three of them polymorphic: <i>Trichostrongylus axei</i>, <i>Trichostrongylus vitrinus</i>, <i>Trichostrongylus capricola</i>, <i>Trichostrongylus colubriformis</i>, <i>Haemonchus contortus</i>, <i>Spiculopteragia spiculoptera</i>/<i>Spiculopteragia mathevossiani</i>, <i>Ostertagia leptospicularis</i>/<i>Ostertagia kolchida</i>, <i>Ostertagia</i> (<i>Grosspiculopteragia</i>) <i>occidentalis</i>, <i>Teladorsagia circumcincta/Teladorsagia trifurcate</i>, <i>Marshallagia marshalli</i>, <i>Nematodirus europaeus</i>, <i>Cooperia oncophora</i>, <i>Capillaria bovis</i>, <i>Oesophagostomum venulosum,</i> and <i>Trichuris ovis</i>. All of them have already been cited in roe deer in Europe, but <i>Marshallagia marshalli</i>, <i>Capillaria bovis,</i> and <i>Ostertagia</i> (<i>Grosspiculopteragia</i>) <i>occidentalis</i> are reported for the first time in Spain in this host. The abomasum was the intestinal section, where the prevalence (98.9%) and mean intensity (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mover><mi mathvariant="normal">x</mi><mo>¯</mo></mover></mrow></semantics></math></inline-formula> = 370.7 ± 374.4 worms/roe deer; range 3–1762) were significantly higher, but no statistically significant differences were found when comparing the sampling areas and age of animals. The animals with lower body weight had a higher parasite load than those in better physical condition, finding, in this case, statistically significant differences (<i>p</i> = 0.0020). Seven genera and 14 species were identified. In the small intestine, 88% of the animals examined presented gastrointestinal nematodes, with an average intensity of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mover><mi mathvariant="normal">x</mi><mo>¯</mo></mover></mrow></semantics></math></inline-formula> = 131.7 ± 225.6 parasites/infected animal, ranging between 4–1254 worms. No statistically significant differences were found when the three parameters studied were compared. Four genera and seven species were identified. In the large intestine/cecum, 78.3% of the examined roe deer presented adult worms, with an average intensity of 6.3 ± 5.5 worms/infected animal; range 1–26 worms. Only statistically significant differences were observed when considering the mean intensity of parasitism and the sampling area (<i>p</i> = 0.0093). Two genera and two species were identified. Several of the species found in the study were studied molecularly, and with the sequences obtained compared with those deposited in GenBank, phylogenetic trees were prepared to determine their taxonomic status. Using coprological techniques, the existing correlation in the shedding of gastrointestinal nematode eggs in roe deer was investigated with that of semi-extensive sheep farms in the same study area to verify the existence of cross-transmission of these parasites between wild and domestic animals. The high values found in the studied parameters show that northern Spain is an area of high-intensity infection for roe deer.https://www.mdpi.com/2076-2615/13/19/3117gastrointestinal nematodesprevalenceintensityspeciesmolecular studiesphylogenetic trees |
| spellingShingle | Sara González María Luisa del Rio Natividad Díez-Baños Angélica Martínez María del Rosario Hidalgo Contribution to the Knowledge of Gastrointestinal Nematodes in Roe Deer (<i>Capreolus capreolus</i>) from the Province of León, Spain: An Epidemiological and Molecular Study Animals gastrointestinal nematodes prevalence intensity species molecular studies phylogenetic trees |
| title | Contribution to the Knowledge of Gastrointestinal Nematodes in Roe Deer (<i>Capreolus capreolus</i>) from the Province of León, Spain: An Epidemiological and Molecular Study |
| title_full | Contribution to the Knowledge of Gastrointestinal Nematodes in Roe Deer (<i>Capreolus capreolus</i>) from the Province of León, Spain: An Epidemiological and Molecular Study |
| title_fullStr | Contribution to the Knowledge of Gastrointestinal Nematodes in Roe Deer (<i>Capreolus capreolus</i>) from the Province of León, Spain: An Epidemiological and Molecular Study |
| title_full_unstemmed | Contribution to the Knowledge of Gastrointestinal Nematodes in Roe Deer (<i>Capreolus capreolus</i>) from the Province of León, Spain: An Epidemiological and Molecular Study |
| title_short | Contribution to the Knowledge of Gastrointestinal Nematodes in Roe Deer (<i>Capreolus capreolus</i>) from the Province of León, Spain: An Epidemiological and Molecular Study |
| title_sort | contribution to the knowledge of gastrointestinal nematodes in roe deer i capreolus capreolus i from the province of leon spain an epidemiological and molecular study |
| topic | gastrointestinal nematodes prevalence intensity species molecular studies phylogenetic trees |
| url | https://www.mdpi.com/2076-2615/13/19/3117 |
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