<i>Holarrhena pubescens</i> Wall. ex G. Don Extracts Inhibit LPS-Irritated Oxidative Stress in Dendritic Cells
<i>Holarrhena pubescens</i> Wall. ex G. Don (<i>H. pubescens</i>), belonging to the <i>Apocynaceae</i> family, is distributed in deciduous forests of the tropical Himalayas. <i>H. pubescens</i> is an important traditional medicinal plant, especially it...
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MDPI AG
2022-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/22/11343 |
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| author | Yinyan Yin Bangyue Zhou Lirong Bai Danni Feng Tao Qin Chunmei Li |
| author_facet | Yinyan Yin Bangyue Zhou Lirong Bai Danni Feng Tao Qin Chunmei Li |
| author_sort | Yinyan Yin |
| collection | DOAJ |
| description | <i>Holarrhena pubescens</i> Wall. ex G. Don (<i>H. pubescens</i>), belonging to the <i>Apocynaceae</i> family, is distributed in deciduous forests of the tropical Himalayas. <i>H. pubescens</i> is an important traditional medicinal plant, especially its seeds and barks. Therefore, we assessed the antioxidant capacity of <i>H. pubescens</i> extracts in Lipopolysaccharide (LPS)-induced dendritic cells (DCs) for sepsis treatment. Our results indicated that <i>H. pubescens</i> extracts with different doses (25 μg/mL, 50 μg/mL, 100 μg/mL) reduced the reactive oxygen species (ROS) level, and weakened the nitric oxide synthases (NOS) activity and nitric oxide (NO) level in LPS (100 ng/mL)-irritated DCs. In addition, <i>H. pubescens</i> extracts decreased the oxidized glutathione (GSSG) production but increased the reduced glutathione (GSH) production, thereby preserving the cellular reductive status owing to the raised GSH/GSSG ratio. Furthermore, <i>H. pubescens</i> extracts strengthened the antioxidant enzymes activity in LPS-induced DCs, such as glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD). Finally, we found that <i>H. pubescens</i> extracts significantly improved the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) and the heme oxygenase 1 (HO–1) in LPS-irritated DCs. These results indicated that <i>H. pubescens</i> extracts suppressed the LPS-irritated oxidative stress in DCs via Nrf2/HO–1 signaling pathway, providing a potential strategy for sepsis therapy. |
| first_indexed | 2024-03-09T18:30:08Z |
| format | Article |
| id | doaj.art-3537e6d155cf44c4877d78489339cf89 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T18:30:08Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-3537e6d155cf44c4877d78489339cf892023-11-24T07:33:46ZengMDPI AGApplied Sciences2076-34172022-11-0112221134310.3390/app122211343<i>Holarrhena pubescens</i> Wall. ex G. Don Extracts Inhibit LPS-Irritated Oxidative Stress in Dendritic CellsYinyan Yin0Bangyue Zhou1Lirong Bai2Danni Feng3Tao Qin4Chunmei Li5College of Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225009, China<i>Holarrhena pubescens</i> Wall. ex G. Don (<i>H. pubescens</i>), belonging to the <i>Apocynaceae</i> family, is distributed in deciduous forests of the tropical Himalayas. <i>H. pubescens</i> is an important traditional medicinal plant, especially its seeds and barks. Therefore, we assessed the antioxidant capacity of <i>H. pubescens</i> extracts in Lipopolysaccharide (LPS)-induced dendritic cells (DCs) for sepsis treatment. Our results indicated that <i>H. pubescens</i> extracts with different doses (25 μg/mL, 50 μg/mL, 100 μg/mL) reduced the reactive oxygen species (ROS) level, and weakened the nitric oxide synthases (NOS) activity and nitric oxide (NO) level in LPS (100 ng/mL)-irritated DCs. In addition, <i>H. pubescens</i> extracts decreased the oxidized glutathione (GSSG) production but increased the reduced glutathione (GSH) production, thereby preserving the cellular reductive status owing to the raised GSH/GSSG ratio. Furthermore, <i>H. pubescens</i> extracts strengthened the antioxidant enzymes activity in LPS-induced DCs, such as glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD). Finally, we found that <i>H. pubescens</i> extracts significantly improved the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) and the heme oxygenase 1 (HO–1) in LPS-irritated DCs. These results indicated that <i>H. pubescens</i> extracts suppressed the LPS-irritated oxidative stress in DCs via Nrf2/HO–1 signaling pathway, providing a potential strategy for sepsis therapy.https://www.mdpi.com/2076-3417/12/22/11343herbal medicines<i>H. pubescens</i>oxidative stressdendritic cells |
| spellingShingle | Yinyan Yin Bangyue Zhou Lirong Bai Danni Feng Tao Qin Chunmei Li <i>Holarrhena pubescens</i> Wall. ex G. Don Extracts Inhibit LPS-Irritated Oxidative Stress in Dendritic Cells Applied Sciences herbal medicines <i>H. pubescens</i> oxidative stress dendritic cells |
| title | <i>Holarrhena pubescens</i> Wall. ex G. Don Extracts Inhibit LPS-Irritated Oxidative Stress in Dendritic Cells |
| title_full | <i>Holarrhena pubescens</i> Wall. ex G. Don Extracts Inhibit LPS-Irritated Oxidative Stress in Dendritic Cells |
| title_fullStr | <i>Holarrhena pubescens</i> Wall. ex G. Don Extracts Inhibit LPS-Irritated Oxidative Stress in Dendritic Cells |
| title_full_unstemmed | <i>Holarrhena pubescens</i> Wall. ex G. Don Extracts Inhibit LPS-Irritated Oxidative Stress in Dendritic Cells |
| title_short | <i>Holarrhena pubescens</i> Wall. ex G. Don Extracts Inhibit LPS-Irritated Oxidative Stress in Dendritic Cells |
| title_sort | i holarrhena pubescens i wall ex g don extracts inhibit lps irritated oxidative stress in dendritic cells |
| topic | herbal medicines <i>H. pubescens</i> oxidative stress dendritic cells |
| url | https://www.mdpi.com/2076-3417/12/22/11343 |
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