Interspecific Signaling Between the Parasitic Plant and the Host Plants Regulate Xylem Vessel Cell Differentiation in Haustoria of Cuscuta campestris
The genus Cuscuta is stem parasitic angiosperms that parasitize a wide range of vascular plants via de novo formation of a distinctive parasitic organ called a haustorium. In the developing haustorium, meristematic cells, which are initiated from the stem cortical tissue, differentiate into haustori...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00193/full |
| _version_ | 1818524031331074048 |
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| author | Yuki Kaga Ryusuke Yokoyama Ryosuke Sano Misato Ohtani Misato Ohtani Taku Demura Takeshi Kuroha Naoki Shinohara Kazuhiko Nishitani Kazuhiko Nishitani |
| author_facet | Yuki Kaga Ryusuke Yokoyama Ryosuke Sano Misato Ohtani Misato Ohtani Taku Demura Takeshi Kuroha Naoki Shinohara Kazuhiko Nishitani Kazuhiko Nishitani |
| author_sort | Yuki Kaga |
| collection | DOAJ |
| description | The genus Cuscuta is stem parasitic angiosperms that parasitize a wide range of vascular plants via de novo formation of a distinctive parasitic organ called a haustorium. In the developing haustorium, meristematic cells, which are initiated from the stem cortical tissue, differentiate into haustorial parenchyma cells, which elongate, penetrate into the host tissues, and finally connect with the host vasculature. This interspecific vasculature connection allows the parasite to uptake water and nutrients from the host plant. Although histological aspects of haustorium development have been studied extensively, the molecular mechanisms underlying vasculature development and the interspecific connection with the host vasculature remain largely unknown. To gain insights into the interspecific cell-to-cell interactions involved in haustorium development, we established an in vitro haustorium induction system for Cuscuta campestris using Arabidopsis thaliana rosette leaves as the host plant tissue. The in vitro induction system was used to show that interaction with host tissue was required for the differentiation of parasite haustorial cells into xylem vessel cells. To further characterize the molecular events occurring during host-dependent xylem vessel cell differentiation in C. campestris, we performed a transcriptome analysis using samples from the in vitro induction system. The results showed that orthologs of genes involved in development and proliferation of vascular stem cells were up-regulated even in the absence of host tissue, whereas orthologs of genes required for xylem vessel cell differentiation were up-regulated only after some haustorial cells had elongated and contacted the host xylem. Consistent results were obtained by another transcriptome analysis of the haustorium development in C. campestris undergoing parasitization of an intact host plant. These findings suggest the involvement of host-derived signals in the regulation of non-autonomous xylem vessel differentiation and suggest that its connection to the host xylem during the haustorium development activates a set of key genes for differentiation into xylem vessel cells. |
| first_indexed | 2024-12-11T05:52:09Z |
| format | Article |
| id | doaj.art-3540e466f74c4bb1b28400480b230bf1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T05:52:09Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-3540e466f74c4bb1b28400480b230bf12022-12-22T01:18:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-03-011110.3389/fpls.2020.00193524693Interspecific Signaling Between the Parasitic Plant and the Host Plants Regulate Xylem Vessel Cell Differentiation in Haustoria of Cuscuta campestrisYuki Kaga0Ryusuke Yokoyama1Ryosuke Sano2Misato Ohtani3Misato Ohtani4Taku Demura5Takeshi Kuroha6Naoki Shinohara7Kazuhiko Nishitani8Kazuhiko Nishitani9Graduate School of Life Sciences, Tohoku University, Sendai, JapanGraduate School of Life Sciences, Tohoku University, Sendai, JapanGraduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, JapanGraduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, JapanGraduate School of Frontier Sciences, The University of Tokyo, Kashiwa, JapanGraduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, JapanGraduate School of Life Sciences, Tohoku University, Sendai, JapanFaculty of Science, Kanagawa University, Hiratsuka, Kanagawa, JapanGraduate School of Life Sciences, Tohoku University, Sendai, JapanFaculty of Science, Kanagawa University, Hiratsuka, Kanagawa, JapanThe genus Cuscuta is stem parasitic angiosperms that parasitize a wide range of vascular plants via de novo formation of a distinctive parasitic organ called a haustorium. In the developing haustorium, meristematic cells, which are initiated from the stem cortical tissue, differentiate into haustorial parenchyma cells, which elongate, penetrate into the host tissues, and finally connect with the host vasculature. This interspecific vasculature connection allows the parasite to uptake water and nutrients from the host plant. Although histological aspects of haustorium development have been studied extensively, the molecular mechanisms underlying vasculature development and the interspecific connection with the host vasculature remain largely unknown. To gain insights into the interspecific cell-to-cell interactions involved in haustorium development, we established an in vitro haustorium induction system for Cuscuta campestris using Arabidopsis thaliana rosette leaves as the host plant tissue. The in vitro induction system was used to show that interaction with host tissue was required for the differentiation of parasite haustorial cells into xylem vessel cells. To further characterize the molecular events occurring during host-dependent xylem vessel cell differentiation in C. campestris, we performed a transcriptome analysis using samples from the in vitro induction system. The results showed that orthologs of genes involved in development and proliferation of vascular stem cells were up-regulated even in the absence of host tissue, whereas orthologs of genes required for xylem vessel cell differentiation were up-regulated only after some haustorial cells had elongated and contacted the host xylem. Consistent results were obtained by another transcriptome analysis of the haustorium development in C. campestris undergoing parasitization of an intact host plant. These findings suggest the involvement of host-derived signals in the regulation of non-autonomous xylem vessel differentiation and suggest that its connection to the host xylem during the haustorium development activates a set of key genes for differentiation into xylem vessel cells.https://www.frontiersin.org/article/10.3389/fpls.2020.00193/fullstem parasitic plantplant-plant interspecific interactionhaustorium developmentxylem vessel differentiationtranscriptome analysis |
| spellingShingle | Yuki Kaga Ryusuke Yokoyama Ryosuke Sano Misato Ohtani Misato Ohtani Taku Demura Takeshi Kuroha Naoki Shinohara Kazuhiko Nishitani Kazuhiko Nishitani Interspecific Signaling Between the Parasitic Plant and the Host Plants Regulate Xylem Vessel Cell Differentiation in Haustoria of Cuscuta campestris Frontiers in Plant Science stem parasitic plant plant-plant interspecific interaction haustorium development xylem vessel differentiation transcriptome analysis |
| title | Interspecific Signaling Between the Parasitic Plant and the Host Plants Regulate Xylem Vessel Cell Differentiation in Haustoria of Cuscuta campestris |
| title_full | Interspecific Signaling Between the Parasitic Plant and the Host Plants Regulate Xylem Vessel Cell Differentiation in Haustoria of Cuscuta campestris |
| title_fullStr | Interspecific Signaling Between the Parasitic Plant and the Host Plants Regulate Xylem Vessel Cell Differentiation in Haustoria of Cuscuta campestris |
| title_full_unstemmed | Interspecific Signaling Between the Parasitic Plant and the Host Plants Regulate Xylem Vessel Cell Differentiation in Haustoria of Cuscuta campestris |
| title_short | Interspecific Signaling Between the Parasitic Plant and the Host Plants Regulate Xylem Vessel Cell Differentiation in Haustoria of Cuscuta campestris |
| title_sort | interspecific signaling between the parasitic plant and the host plants regulate xylem vessel cell differentiation in haustoria of cuscuta campestris |
| topic | stem parasitic plant plant-plant interspecific interaction haustorium development xylem vessel differentiation transcriptome analysis |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00193/full |
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