Tracing Asian seabass individuals to single fish farms using microsatellites.
Traceability through physical labels is well established, but it is not highly reliable as physical labels can be easily changed or lost. Application of DNA markers to the traceability of food plays an increasingly important role for consumer protection and confidence building. In this study, we tes...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285169/pdf/?tool=EBI |
| _version_ | 1818398828353552384 |
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| author | Gen Hua Yue Jun Hong Xia Peng Liu Feng Liu Fei Sun Grace Lin |
| author_facet | Gen Hua Yue Jun Hong Xia Peng Liu Feng Liu Fei Sun Grace Lin |
| author_sort | Gen Hua Yue |
| collection | DOAJ |
| description | Traceability through physical labels is well established, but it is not highly reliable as physical labels can be easily changed or lost. Application of DNA markers to the traceability of food plays an increasingly important role for consumer protection and confidence building. In this study, we tested the efficiency of 16 polymorphic microsatellites and their combinations for tracing 368 fish to four populations where they originated. Using the maximum likelihood and Bayesian methods, three most efficient microsatellites were required to assign over 95% of fish to the correct populations. Selection of markers based on the assignment score estimated with the software WHICHLOCI was most effective in choosing markers for individual assignment, followed by the selection based on the allele number of individual markers. By combining rapid DNA extraction, and high-throughput genotyping of selected microsatellites, it is possible to conduct routine genetic traceability with high accuracy in Asian seabass. |
| first_indexed | 2024-12-14T07:10:59Z |
| format | Article |
| id | doaj.art-3541c8894f6a47808484670b3f174601 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T07:10:59Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3541c8894f6a47808484670b3f1746012022-12-21T23:11:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5272110.1371/journal.pone.0052721Tracing Asian seabass individuals to single fish farms using microsatellites.Gen Hua YueJun Hong XiaPeng LiuFeng LiuFei SunGrace LinTraceability through physical labels is well established, but it is not highly reliable as physical labels can be easily changed or lost. Application of DNA markers to the traceability of food plays an increasingly important role for consumer protection and confidence building. In this study, we tested the efficiency of 16 polymorphic microsatellites and their combinations for tracing 368 fish to four populations where they originated. Using the maximum likelihood and Bayesian methods, three most efficient microsatellites were required to assign over 95% of fish to the correct populations. Selection of markers based on the assignment score estimated with the software WHICHLOCI was most effective in choosing markers for individual assignment, followed by the selection based on the allele number of individual markers. By combining rapid DNA extraction, and high-throughput genotyping of selected microsatellites, it is possible to conduct routine genetic traceability with high accuracy in Asian seabass.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285169/pdf/?tool=EBI |
| spellingShingle | Gen Hua Yue Jun Hong Xia Peng Liu Feng Liu Fei Sun Grace Lin Tracing Asian seabass individuals to single fish farms using microsatellites. PLoS ONE |
| title | Tracing Asian seabass individuals to single fish farms using microsatellites. |
| title_full | Tracing Asian seabass individuals to single fish farms using microsatellites. |
| title_fullStr | Tracing Asian seabass individuals to single fish farms using microsatellites. |
| title_full_unstemmed | Tracing Asian seabass individuals to single fish farms using microsatellites. |
| title_short | Tracing Asian seabass individuals to single fish farms using microsatellites. |
| title_sort | tracing asian seabass individuals to single fish farms using microsatellites |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285169/pdf/?tool=EBI |
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