Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication
Abstract Background Endoreplication is involved in the development and function of many organs, the pathologic process of several diseases. However, the metabolic underpinnings and regulation of endoreplication have yet to be well clarified. Results Here, we showed that a zinc transporter fear-of-in...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-04-01
|
| Series: | BMC Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12915-023-01588-0 |
| _version_ | 1797840842137796608 |
|---|---|
| author | Xiaowen Ji Jiajia Gao Tian Wei Li Jin Guiran Xiao |
| author_facet | Xiaowen Ji Jiajia Gao Tian Wei Li Jin Guiran Xiao |
| author_sort | Xiaowen Ji |
| collection | DOAJ |
| description | Abstract Background Endoreplication is involved in the development and function of many organs, the pathologic process of several diseases. However, the metabolic underpinnings and regulation of endoreplication have yet to be well clarified. Results Here, we showed that a zinc transporter fear-of-intimacy (foi) is necessary for Drosophila fat body endoreplication. foi knockdown in the fat body led to fat body cell nuclei failure to attain standard size, decreased fat body size and pupal lethality. These phenotypes could be modulated by either altered expression of genes involved in zinc metabolism or intervention of dietary zinc levels. Further studies indicated that the intracellular depletion of zinc caused by foi knockdown results in oxidative stress, which activates the ROS-JNK signaling pathway, and then inhibits the expression of Myc, which is required for tissue endoreplication and larval growth in Drosophila. Conclusions Our results indicated that FOI is critical in coordinating fat body endoreplication and larval growth in Drosophila. Our study provides a novel insight into the relationship between zinc and endoreplication in insects and may provide a reference for relevant mammalian studies. |
| first_indexed | 2024-04-09T16:21:20Z |
| format | Article |
| id | doaj.art-35423266c6204897b2dfae15dc9fc0ff |
| institution | Directory Open Access Journal |
| issn | 1741-7007 |
| language | English |
| last_indexed | 2024-04-09T16:21:20Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biology |
| spelling | doaj.art-35423266c6204897b2dfae15dc9fc0ff2023-04-23T11:28:14ZengBMCBMC Biology1741-70072023-04-0121111810.1186/s12915-023-01588-0Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplicationXiaowen Ji0Jiajia Gao1Tian Wei2Li Jin3Guiran Xiao4China Light Industry Key Laboratory of Meat Microbial Control and Utilization, Hefei University of TechnologyChina Light Industry Key Laboratory of Meat Microbial Control and Utilization, Hefei University of TechnologyChina Light Industry Key Laboratory of Meat Microbial Control and Utilization, Hefei University of TechnologyChina Light Industry Key Laboratory of Meat Microbial Control and Utilization, Hefei University of TechnologyChina Light Industry Key Laboratory of Meat Microbial Control and Utilization, Hefei University of TechnologyAbstract Background Endoreplication is involved in the development and function of many organs, the pathologic process of several diseases. However, the metabolic underpinnings and regulation of endoreplication have yet to be well clarified. Results Here, we showed that a zinc transporter fear-of-intimacy (foi) is necessary for Drosophila fat body endoreplication. foi knockdown in the fat body led to fat body cell nuclei failure to attain standard size, decreased fat body size and pupal lethality. These phenotypes could be modulated by either altered expression of genes involved in zinc metabolism or intervention of dietary zinc levels. Further studies indicated that the intracellular depletion of zinc caused by foi knockdown results in oxidative stress, which activates the ROS-JNK signaling pathway, and then inhibits the expression of Myc, which is required for tissue endoreplication and larval growth in Drosophila. Conclusions Our results indicated that FOI is critical in coordinating fat body endoreplication and larval growth in Drosophila. Our study provides a novel insight into the relationship between zinc and endoreplication in insects and may provide a reference for relevant mammalian studies.https://doi.org/10.1186/s12915-023-01588-0FOIZincFat bodyEndoreplicationJNKMyc |
| spellingShingle | Xiaowen Ji Jiajia Gao Tian Wei Li Jin Guiran Xiao Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication BMC Biology FOI Zinc Fat body Endoreplication JNK Myc |
| title | Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication |
| title_full | Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication |
| title_fullStr | Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication |
| title_full_unstemmed | Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication |
| title_short | Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication |
| title_sort | fear of intimacy mediated zinc transport is required for drosophila fat body endoreplication |
| topic | FOI Zinc Fat body Endoreplication JNK Myc |
| url | https://doi.org/10.1186/s12915-023-01588-0 |
| work_keys_str_mv | AT xiaowenji fearofintimacymediatedzinctransportisrequiredfordrosophilafatbodyendoreplication AT jiajiagao fearofintimacymediatedzinctransportisrequiredfordrosophilafatbodyendoreplication AT tianwei fearofintimacymediatedzinctransportisrequiredfordrosophilafatbodyendoreplication AT lijin fearofintimacymediatedzinctransportisrequiredfordrosophilafatbodyendoreplication AT guiranxiao fearofintimacymediatedzinctransportisrequiredfordrosophilafatbodyendoreplication |