Investigation of Anticancer Properties of Monoterpene-Aminopyrimidine Hybrids on A2780 Ovarian Cancer Cells
The present study aimed to characterize the antiproliferative and antimetastatic properties of two recently synthesized monoterpene-aminopyrimidine hybrids (<b>1</b> and <b>2</b>) on A2780 ovary cancer cells. Both agents exerted a more pronounced cell growth inhibitory action...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/13/10581 |
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| author | Viktória Nagy Raji Mounir Gábor J. Szebeni Zsolt Szakonyi Nikolett Gémes Renáta Minorics Péter Germán István Zupkó |
| author_facet | Viktória Nagy Raji Mounir Gábor J. Szebeni Zsolt Szakonyi Nikolett Gémes Renáta Minorics Péter Germán István Zupkó |
| author_sort | Viktória Nagy |
| collection | DOAJ |
| description | The present study aimed to characterize the antiproliferative and antimetastatic properties of two recently synthesized monoterpene-aminopyrimidine hybrids (<b>1</b> and <b>2</b>) on A2780 ovary cancer cells. Both agents exerted a more pronounced cell growth inhibitory action than the reference agent cisplatin, as determined by the MTT assay. Tumor selectivity was assessed using non-cancerous fibroblast cells. Hybrids <b>1</b> and <b>2</b> induced changes in cell morphology and membrane integrity in A2780 cells, as evidenced by Hoechst 33258–propidium iodide fluorescent staining. Cell cycle analysis by flow cytometry revealed substantial changes in the distribution of A2780 ovarian cancer cells, with an increased rate in the subG1 and G2/M phases, at the expense of the G1 cell population. Moreover, the tested molecules accelerated tubulin polymerization in a cell-free in vitro system. The antimetastatic properties of both tested compounds were investigated by wound healing and Boyden chamber assays after 24 and 48 h of incubation. Treatment with <b>1</b> and <b>2</b> resulted in time- and concentration-dependent inhibition of migration and invasion of A2780 cancer cells. These results support that the tested agents may be worth of further investigation as promising anticancer drug candidates. |
| first_indexed | 2024-03-11T01:39:50Z |
| format | Article |
| id | doaj.art-3543d74e3e7647bb811aea7228bb4dd0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:39:50Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3543d74e3e7647bb811aea7228bb4dd02023-11-18T16:40:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124131058110.3390/ijms241310581Investigation of Anticancer Properties of Monoterpene-Aminopyrimidine Hybrids on A2780 Ovarian Cancer CellsViktória Nagy0Raji Mounir1Gábor J. Szebeni2Zsolt Szakonyi3Nikolett Gémes4Renáta Minorics5Péter Germán6István Zupkó7Institute of Pharmacodynamics and Biopharmacy, University of Szeged, H-6720 Szeged, HungaryInstitute of Pharmaceutical Chemistry, University of Szeged, H-6720 Szeged, HungaryLaboratory of Functional Genomics, Eötvös Loránd Research Network Biological Research Centre, Institute of Genetics, H-6726 Szeged, HungaryInstitute of Pharmaceutical Chemistry, University of Szeged, H-6720 Szeged, HungaryLaboratory of Functional Genomics, Eötvös Loránd Research Network Biological Research Centre, Institute of Genetics, H-6726 Szeged, HungaryInstitute of Pharmacodynamics and Biopharmacy, University of Szeged, H-6720 Szeged, HungaryInstitute of Pharmacodynamics and Biopharmacy, University of Szeged, H-6720 Szeged, HungaryInstitute of Pharmacodynamics and Biopharmacy, University of Szeged, H-6720 Szeged, HungaryThe present study aimed to characterize the antiproliferative and antimetastatic properties of two recently synthesized monoterpene-aminopyrimidine hybrids (<b>1</b> and <b>2</b>) on A2780 ovary cancer cells. Both agents exerted a more pronounced cell growth inhibitory action than the reference agent cisplatin, as determined by the MTT assay. Tumor selectivity was assessed using non-cancerous fibroblast cells. Hybrids <b>1</b> and <b>2</b> induced changes in cell morphology and membrane integrity in A2780 cells, as evidenced by Hoechst 33258–propidium iodide fluorescent staining. Cell cycle analysis by flow cytometry revealed substantial changes in the distribution of A2780 ovarian cancer cells, with an increased rate in the subG1 and G2/M phases, at the expense of the G1 cell population. Moreover, the tested molecules accelerated tubulin polymerization in a cell-free in vitro system. The antimetastatic properties of both tested compounds were investigated by wound healing and Boyden chamber assays after 24 and 48 h of incubation. Treatment with <b>1</b> and <b>2</b> resulted in time- and concentration-dependent inhibition of migration and invasion of A2780 cancer cells. These results support that the tested agents may be worth of further investigation as promising anticancer drug candidates.https://www.mdpi.com/1422-0067/24/13/10581monoterpeneaminoalcoholantiproliferativeG2/M arrestantimetastaticovarian cancer |
| spellingShingle | Viktória Nagy Raji Mounir Gábor J. Szebeni Zsolt Szakonyi Nikolett Gémes Renáta Minorics Péter Germán István Zupkó Investigation of Anticancer Properties of Monoterpene-Aminopyrimidine Hybrids on A2780 Ovarian Cancer Cells International Journal of Molecular Sciences monoterpene aminoalcohol antiproliferative G2/M arrest antimetastatic ovarian cancer |
| title | Investigation of Anticancer Properties of Monoterpene-Aminopyrimidine Hybrids on A2780 Ovarian Cancer Cells |
| title_full | Investigation of Anticancer Properties of Monoterpene-Aminopyrimidine Hybrids on A2780 Ovarian Cancer Cells |
| title_fullStr | Investigation of Anticancer Properties of Monoterpene-Aminopyrimidine Hybrids on A2780 Ovarian Cancer Cells |
| title_full_unstemmed | Investigation of Anticancer Properties of Monoterpene-Aminopyrimidine Hybrids on A2780 Ovarian Cancer Cells |
| title_short | Investigation of Anticancer Properties of Monoterpene-Aminopyrimidine Hybrids on A2780 Ovarian Cancer Cells |
| title_sort | investigation of anticancer properties of monoterpene aminopyrimidine hybrids on a2780 ovarian cancer cells |
| topic | monoterpene aminoalcohol antiproliferative G2/M arrest antimetastatic ovarian cancer |
| url | https://www.mdpi.com/1422-0067/24/13/10581 |
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