Characterization of Arabidopsis seedlings growth and development under trehalose feeding
Trehalose is the alpha, alpha-1,1-linked glucose disaccharide. Its metabolism is found in a wide variety of organisms and is seen as evolutionary old. Trehalose metabolites are however present at only very low concentrations and their role in plants are not understood. The physiological effects of 1...
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2010-01-01
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| Series: | Journal of Cell and Molecular Research |
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| Online Access: | https://jcmr.um.ac.ir/article_25833_465287f8e5fbc05432b607f73dbb5162.pdf |
| _version_ | 1818332483264970752 |
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| author | Mahnaz Aghdasi Henriette Schluepmann Sjef Smeekens |
| author_facet | Mahnaz Aghdasi Henriette Schluepmann Sjef Smeekens |
| author_sort | Mahnaz Aghdasi |
| collection | DOAJ |
| description | Trehalose is the alpha, alpha-1,1-linked glucose disaccharide. Its metabolism is found in a wide variety of organisms and is seen as evolutionary old. Trehalose metabolites are however present at only very low concentrations and their role in plants are not understood. The physiological effects of 100 mM trehalose on growth and carbon allocation in seedlings are characterized in this paper. Trehalose feeding to Arabidopsis thaliana elicits strong responses. On 100 mM trehalose, seedlings germinate and extend cotyledons but fail to develop primary leaves. The primary roots do not grow beyond 2-3 mm and there is not any starch in root tips. In light, growth arrest on 100 mM trehalose can be rescued by exogenous supply of metabolisable sugar. Trehalose feeding results in anthocyanin accumulation and chlorophyll reduction. Trehalose causes cells of the root extension zone to swell and lysis. Trehalase expression analysis showed, WT seedlings grown on trehalose have 10-fold induced AtTRE1 expression compared to sorbitol treatment. |
| first_indexed | 2024-12-13T13:36:27Z |
| format | Article |
| id | doaj.art-354ad137e85c404eb1e46746b6825eaf |
| institution | Directory Open Access Journal |
| issn | 2008-9147 2717-3364 |
| language | English |
| last_indexed | 2024-12-13T13:36:27Z |
| publishDate | 2010-01-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | Journal of Cell and Molecular Research |
| spelling | doaj.art-354ad137e85c404eb1e46746b6825eaf2022-12-21T23:43:47ZengFerdowsi University of MashhadJournal of Cell and Molecular Research2008-91472717-33642010-01-01211910.22067/jcmr.v2i1.328225833Characterization of Arabidopsis seedlings growth and development under trehalose feedingMahnaz AghdasiHenriette Schluepmann0Sjef Smeekens1Utrecht UniversityUtrecht UniversityTrehalose is the alpha, alpha-1,1-linked glucose disaccharide. Its metabolism is found in a wide variety of organisms and is seen as evolutionary old. Trehalose metabolites are however present at only very low concentrations and their role in plants are not understood. The physiological effects of 100 mM trehalose on growth and carbon allocation in seedlings are characterized in this paper. Trehalose feeding to Arabidopsis thaliana elicits strong responses. On 100 mM trehalose, seedlings germinate and extend cotyledons but fail to develop primary leaves. The primary roots do not grow beyond 2-3 mm and there is not any starch in root tips. In light, growth arrest on 100 mM trehalose can be rescued by exogenous supply of metabolisable sugar. Trehalose feeding results in anthocyanin accumulation and chlorophyll reduction. Trehalose causes cells of the root extension zone to swell and lysis. Trehalase expression analysis showed, WT seedlings grown on trehalose have 10-fold induced AtTRE1 expression compared to sorbitol treatment.https://jcmr.um.ac.ir/article_25833_465287f8e5fbc05432b607f73dbb5162.pdftrehalosearabidopsis thalianaanthocyanin accumulationt6pcarbon allocation |
| spellingShingle | Mahnaz Aghdasi Henriette Schluepmann Sjef Smeekens Characterization of Arabidopsis seedlings growth and development under trehalose feeding Journal of Cell and Molecular Research trehalose arabidopsis thaliana anthocyanin accumulation t6p carbon allocation |
| title | Characterization of Arabidopsis seedlings growth and development under trehalose feeding |
| title_full | Characterization of Arabidopsis seedlings growth and development under trehalose feeding |
| title_fullStr | Characterization of Arabidopsis seedlings growth and development under trehalose feeding |
| title_full_unstemmed | Characterization of Arabidopsis seedlings growth and development under trehalose feeding |
| title_short | Characterization of Arabidopsis seedlings growth and development under trehalose feeding |
| title_sort | characterization of arabidopsis seedlings growth and development under trehalose feeding |
| topic | trehalose arabidopsis thaliana anthocyanin accumulation t6p carbon allocation |
| url | https://jcmr.um.ac.ir/article_25833_465287f8e5fbc05432b607f73dbb5162.pdf |
| work_keys_str_mv | AT mahnazaghdasi characterizationofarabidopsisseedlingsgrowthanddevelopmentundertrehalosefeeding AT henrietteschluepmann characterizationofarabidopsisseedlingsgrowthanddevelopmentundertrehalosefeeding AT sjefsmeekens characterizationofarabidopsisseedlingsgrowthanddevelopmentundertrehalosefeeding |