Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran
<em><strong>Objective(s):</strong></em> Staphylococcus aureus can cause several infections. Its capability to form biofilm has been reported to be a vital property involved in the bacteria’s pathogenesis. Various genes contributing to biofilm formation have not yet been compl...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_15036_203bad70fc0ad3d90e65449010ece492.pdf |
| _version_ | 1818327640029790208 |
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| author | Hiva Kadkhoda Zohreh Ghalavand Bahram Nikmanesh Mansoor Kodori Hamidreza Houri Donya Taghizadeh Maleki Ali Karimi Bavandpour Gita Eslami |
| author_facet | Hiva Kadkhoda Zohreh Ghalavand Bahram Nikmanesh Mansoor Kodori Hamidreza Houri Donya Taghizadeh Maleki Ali Karimi Bavandpour Gita Eslami |
| author_sort | Hiva Kadkhoda |
| collection | DOAJ |
| description | <em><strong>Objective(s):</strong></em> Staphylococcus aureus can cause several infections. Its capability to form biofilm has been reported to be a vital property involved in the bacteria’s pathogenesis. Various genes contributing to biofilm formation have not yet been completely clarified. This study was designed to evaluate the factors influencing adherence and biofilm formation in S. aureus isolated from paediatric patients.<br /><em><strong>Materials and Methods:</strong></em> One hundred and ninety-seven S. aureus isolates were obtained from pediatric patients and confirmed with phenotypic and molecular examinations. Antimicrobial susceptibility testing and biofilm formation were evaluated using standard methods. The genes encoding adhesion and virulence factors were investigated by the PCR method. <br /><em><strong>Results:</strong></em> The most efficient antibiotics against S. aureus isolates were vancomycin and linezolid. Approximately, 54.2% of MSSA and 85.6% of MRSA isolates were biofilm producers according to the microtiter test. Our analysis indicated that MRSA isolates are better able to form biofilm compared with MSSA isolates. All isolates harbored clfA, fnbpA, icaA, icaB, icaC, and icaD, while clfB, fnbB, hlg, and pvl were detected in 99.5%, 42.1%, 97.5%, and 5.6% of isolates, respectively. In addition, a significant difference was found in fnhB gene and biofilm formation.<br /><em><strong>Conclusion:</strong></em> Our findings showed a significant correlation between mecA and pvl genes and MRSA and biofilm formation in S. aureus isolates. Additionally, this study indicated the significant role of the fnhB gene as a major marker for S. aureus biofilm formation. Therefore, further experiments are warranted to exactly elucidate the function of the fnhB gene in the formation of biofilm. |
| first_indexed | 2024-12-13T12:19:29Z |
| format | Article |
| id | doaj.art-354d457cd6b344b4a0c21be28fce197c |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-13T12:19:29Z |
| publishDate | 2020-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-354d457cd6b344b4a0c21be28fce197c2022-12-21T23:46:36ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742020-05-0123569169810.22038/ijbms.2020.36299.864415036Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, IranHiva Kadkhoda0Zohreh Ghalavand1Bahram Nikmanesh2Mansoor Kodori3Hamidreza Houri4Donya Taghizadeh Maleki5Ali Karimi Bavandpour6Gita Eslami7Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Bacteriology, Faculty of Medical Science, Tarbiat Modares University, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran<em><strong>Objective(s):</strong></em> Staphylococcus aureus can cause several infections. Its capability to form biofilm has been reported to be a vital property involved in the bacteria’s pathogenesis. Various genes contributing to biofilm formation have not yet been completely clarified. This study was designed to evaluate the factors influencing adherence and biofilm formation in S. aureus isolated from paediatric patients.<br /><em><strong>Materials and Methods:</strong></em> One hundred and ninety-seven S. aureus isolates were obtained from pediatric patients and confirmed with phenotypic and molecular examinations. Antimicrobial susceptibility testing and biofilm formation were evaluated using standard methods. The genes encoding adhesion and virulence factors were investigated by the PCR method. <br /><em><strong>Results:</strong></em> The most efficient antibiotics against S. aureus isolates were vancomycin and linezolid. Approximately, 54.2% of MSSA and 85.6% of MRSA isolates were biofilm producers according to the microtiter test. Our analysis indicated that MRSA isolates are better able to form biofilm compared with MSSA isolates. All isolates harbored clfA, fnbpA, icaA, icaB, icaC, and icaD, while clfB, fnbB, hlg, and pvl were detected in 99.5%, 42.1%, 97.5%, and 5.6% of isolates, respectively. In addition, a significant difference was found in fnhB gene and biofilm formation.<br /><em><strong>Conclusion:</strong></em> Our findings showed a significant correlation between mecA and pvl genes and MRSA and biofilm formation in S. aureus isolates. Additionally, this study indicated the significant role of the fnhB gene as a major marker for S. aureus biofilm formation. Therefore, further experiments are warranted to exactly elucidate the function of the fnhB gene in the formation of biofilm.http://ijbms.mums.ac.ir/article_15036_203bad70fc0ad3d90e65449010ece492.pdfbiofilmfibronectin binding-proteinsmrsamscrammspediatricstaphylococcus aureusvirulence factors |
| spellingShingle | Hiva Kadkhoda Zohreh Ghalavand Bahram Nikmanesh Mansoor Kodori Hamidreza Houri Donya Taghizadeh Maleki Ali Karimi Bavandpour Gita Eslami Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran Iranian Journal of Basic Medical Sciences biofilm fibronectin binding-proteins mrsa mscramms pediatric staphylococcus aureus virulence factors |
| title | Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran |
| title_full | Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran |
| title_fullStr | Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran |
| title_full_unstemmed | Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran |
| title_short | Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran |
| title_sort | characterization of biofilm formation and virulence factors of staphylococcus aureus isolates from paediatric patients in tehran iran |
| topic | biofilm fibronectin binding-proteins mrsa mscramms pediatric staphylococcus aureus virulence factors |
| url | http://ijbms.mums.ac.ir/article_15036_203bad70fc0ad3d90e65449010ece492.pdf |
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