Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus
Objective(s): The effect of litter size and suckling intensity on the expression of KiSS-1 mRNA in the arcuate nucleus (ARC) of rats were evaluated. Materials and Methods: Thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. Lactating rats w...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2014-08-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/pdf_3194_c67a66bab152538c1bb0ae5eac1a0f14.html |
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| author | Atefeh Noroozi Mohammad Reza Jafarzadeh Shirazi Mohammad Javad Zamiri Amin Tamadon Amir Akhlaghi Nader Tanideh Ali Moghadam Ali Niazi |
| author_facet | Atefeh Noroozi Mohammad Reza Jafarzadeh Shirazi Mohammad Javad Zamiri Amin Tamadon Amir Akhlaghi Nader Tanideh Ali Moghadam Ali Niazi |
| author_sort | Atefeh Noroozi |
| collection | DOAJ |
| description | Objective(s): The effect of litter size and suckling intensity on the expression of KiSS-1 mRNA in the arcuate nucleus (ARC) of rats were evaluated.
Materials and Methods: Thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. Lactating rats were allotted to eight equal groups. In three groups, litter size was adjusted to 5, 10, or 15 pups upon parturition and allowed to suckle their pups continuously by 8 days postpartum. In the other three groups, litter size was adjusted to five upon birth; the pups were separated from the dams for 6 hr on day 8 postpartum, after which the pups were allowed to suckle their dams for 2.5, 5, or 7.5 min prior to killing the dams. Two groups of lactating rats with either 10 or 15 pups were separated from their pups for 6 hr on day 8 postpartum, after which the pups were allowed to suckle their dams for 5 min before the dams were killed on day 8 postpartum. The ARC was removed and the expression of KiSS-1 mRNA was evaluated, using real-time PCR.
Results: The expression of KiSS-1 mRNA in the ARC was decreased as the litter size and intensity of suckling stimulus were increased. The effect of suckling intensity on the expression of KiSS-1 mRNA was more pronounced than that of litter size.
Conclusion: Increased litter size and suckling intensity decreased KiSS-1 mRNA expression in the ARC which may contribute to lactation anestrus in rat. |
| first_indexed | 2024-12-22T10:33:48Z |
| format | Article |
| id | doaj.art-3553cd9d97954fd7866345c47256d933 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-22T10:33:48Z |
| publishDate | 2014-08-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-3553cd9d97954fd7866345c47256d9332022-12-21T18:29:15ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742014-08-011786006053194Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleusAtefeh Noroozi0Mohammad Reza Jafarzadeh Shirazi1Mohammad Javad Zamiri2Amin Tamadon3Amir Akhlaghi4Nader Tanideh5Ali Moghadam6Ali Niazi7Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, IranDepartment of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, IranDepartment of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, IranInfertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, IranDepartment of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Biotechnology Institute, College of Agriculture, Shiraz University, Shiraz, IranBiotechnology Institute, College of Agriculture, Shiraz University, Shiraz, IranBiotechnology Institute, College of Agriculture, Shiraz University, Shiraz, IranObjective(s): The effect of litter size and suckling intensity on the expression of KiSS-1 mRNA in the arcuate nucleus (ARC) of rats were evaluated. Materials and Methods: Thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. Lactating rats were allotted to eight equal groups. In three groups, litter size was adjusted to 5, 10, or 15 pups upon parturition and allowed to suckle their pups continuously by 8 days postpartum. In the other three groups, litter size was adjusted to five upon birth; the pups were separated from the dams for 6 hr on day 8 postpartum, after which the pups were allowed to suckle their dams for 2.5, 5, or 7.5 min prior to killing the dams. Two groups of lactating rats with either 10 or 15 pups were separated from their pups for 6 hr on day 8 postpartum, after which the pups were allowed to suckle their dams for 5 min before the dams were killed on day 8 postpartum. The ARC was removed and the expression of KiSS-1 mRNA was evaluated, using real-time PCR. Results: The expression of KiSS-1 mRNA in the ARC was decreased as the litter size and intensity of suckling stimulus were increased. The effect of suckling intensity on the expression of KiSS-1 mRNA was more pronounced than that of litter size. Conclusion: Increased litter size and suckling intensity decreased KiSS-1 mRNA expression in the ARC which may contribute to lactation anestrus in rat.http://ijbms.mums.ac.ir/pdf_3194_c67a66bab152538c1bb0ae5eac1a0f14.htmlArcuate nucleus KiSS-1 mRNA Lactating rat Litter size Suckling intensity |
| spellingShingle | Atefeh Noroozi Mohammad Reza Jafarzadeh Shirazi Mohammad Javad Zamiri Amin Tamadon Amir Akhlaghi Nader Tanideh Ali Moghadam Ali Niazi Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus Iranian Journal of Basic Medical Sciences Arcuate nucleus KiSS-1 mRNA Lactating rat Litter size Suckling intensity |
| title | Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus |
| title_full | Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus |
| title_fullStr | Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus |
| title_full_unstemmed | Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus |
| title_short | Increased litter size and suckling intensity inhibit KiSS-1 mRNA expression in rat arcuate nucleus |
| title_sort | increased litter size and suckling intensity inhibit kiss 1 mrna expression in rat arcuate nucleus |
| topic | Arcuate nucleus KiSS-1 mRNA Lactating rat Litter size Suckling intensity |
| url | http://ijbms.mums.ac.ir/pdf_3194_c67a66bab152538c1bb0ae5eac1a0f14.html |
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