Low-level laser therapy associated to a resistance training protocol on bone tissue in diabetic rats
ABSTRACT Objective The present study aimed to evaluate the in vivo response of a resistance training and low-level laser therapy (LLLT) on tibias and femurs of rats with diabetes mellitus (DM). Materials and methods Forty male Wistar rats were randomly distributed into four experimental groups:...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Brazilian Society of Endocrinology and Metabolism
2016-08-01
|
| Series: | Archives of Endocrinology and Metabolism |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972016000500457&lng=en&tlng=en |
| _version_ | 1818493382775799808 |
|---|---|
| author | Tatiane Lopes Patrocínio-Silva André Moreira Fogaça de Souza Raul Loppi Goulart Carolina Fuirini Pegorari Jussan Rodrigues Oliveira Kelly Rossetti Fernandes Angela Maria Paiva Magri Rosa Maria Rodrigues Pereira Daniel Araki Ribeiro Márcia Regina Nagaoka Ana Claudia Muniz Rennó |
| author_facet | Tatiane Lopes Patrocínio-Silva André Moreira Fogaça de Souza Raul Loppi Goulart Carolina Fuirini Pegorari Jussan Rodrigues Oliveira Kelly Rossetti Fernandes Angela Maria Paiva Magri Rosa Maria Rodrigues Pereira Daniel Araki Ribeiro Márcia Regina Nagaoka Ana Claudia Muniz Rennó |
| author_sort | Tatiane Lopes Patrocínio-Silva |
| collection | DOAJ |
| description | ABSTRACT Objective The present study aimed to evaluate the in vivo response of a resistance training and low-level laser therapy (LLLT) on tibias and femurs of rats with diabetes mellitus (DM). Materials and methods Forty male Wistar rats were randomly distributed into four experimental groups: control group (CG), diabetic group (DG), diabetic trained group (TG) and diabetic trained and laser irradiated group (TLG). DM was induced by streptozotocin (STZ) and after two weeks laser and resistance training started, performed for 24 sessions, during eight weeks. At the end of the experiment, animals were euthanized and tibias and femurs were removed for analysis. Histological, histomorphometrical, immunohistochemistry and mechanical analyses were performed. Results Trained groups, with or without laser irradiation, showed increased cortical area, bone density and biomechanical properties. The immunohistochemical analysis revealed that TG and TLG demonstrated an increased RUNX2 expression. RANK-L immunoexpression was similar for all experimental groups. Conclusion In conclusion, it can be suggested that the resistance exercise program stimulated bone metabolism, culminating in increased cortical tibial area, bone mineral content, bone mineral density and biomechanical properties. Furthermore, the association of physical exercises and LLLT produced higher values for bone mineral content and stiffness. Consequently, these data highlight the potential of physical exercise in the management of bone loss due to DM and the possible extra osteogenic stimulus offered by lasertherapy. Further long-term studies should be carried out to provide additional information. |
| first_indexed | 2024-12-10T17:54:25Z |
| format | Article |
| id | doaj.art-355887ff92404079a9afa9095c366355 |
| institution | Directory Open Access Journal |
| issn | 2359-4292 |
| language | English |
| last_indexed | 2024-12-10T17:54:25Z |
| publishDate | 2016-08-01 |
| publisher | Brazilian Society of Endocrinology and Metabolism |
| record_format | Article |
| series | Archives of Endocrinology and Metabolism |
| spelling | doaj.art-355887ff92404079a9afa9095c3663552022-12-22T01:38:58ZengBrazilian Society of Endocrinology and MetabolismArchives of Endocrinology and Metabolism2359-42922016-08-0160545746410.1590/2359-3997000000190S2359-39972016000500457Low-level laser therapy associated to a resistance training protocol on bone tissue in diabetic ratsTatiane Lopes Patrocínio-SilvaAndré Moreira Fogaça de SouzaRaul Loppi GoulartCarolina Fuirini PegorariJussan Rodrigues OliveiraKelly Rossetti FernandesAngela Maria Paiva MagriRosa Maria Rodrigues PereiraDaniel Araki RibeiroMárcia Regina NagaokaAna Claudia Muniz RennóABSTRACT Objective The present study aimed to evaluate the in vivo response of a resistance training and low-level laser therapy (LLLT) on tibias and femurs of rats with diabetes mellitus (DM). Materials and methods Forty male Wistar rats were randomly distributed into four experimental groups: control group (CG), diabetic group (DG), diabetic trained group (TG) and diabetic trained and laser irradiated group (TLG). DM was induced by streptozotocin (STZ) and after two weeks laser and resistance training started, performed for 24 sessions, during eight weeks. At the end of the experiment, animals were euthanized and tibias and femurs were removed for analysis. Histological, histomorphometrical, immunohistochemistry and mechanical analyses were performed. Results Trained groups, with or without laser irradiation, showed increased cortical area, bone density and biomechanical properties. The immunohistochemical analysis revealed that TG and TLG demonstrated an increased RUNX2 expression. RANK-L immunoexpression was similar for all experimental groups. Conclusion In conclusion, it can be suggested that the resistance exercise program stimulated bone metabolism, culminating in increased cortical tibial area, bone mineral content, bone mineral density and biomechanical properties. Furthermore, the association of physical exercises and LLLT produced higher values for bone mineral content and stiffness. Consequently, these data highlight the potential of physical exercise in the management of bone loss due to DM and the possible extra osteogenic stimulus offered by lasertherapy. Further long-term studies should be carried out to provide additional information.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972016000500457&lng=en&tlng=enLow-level laser therapyresistance trainingdiabetes mellitusbone |
| spellingShingle | Tatiane Lopes Patrocínio-Silva André Moreira Fogaça de Souza Raul Loppi Goulart Carolina Fuirini Pegorari Jussan Rodrigues Oliveira Kelly Rossetti Fernandes Angela Maria Paiva Magri Rosa Maria Rodrigues Pereira Daniel Araki Ribeiro Márcia Regina Nagaoka Ana Claudia Muniz Rennó Low-level laser therapy associated to a resistance training protocol on bone tissue in diabetic rats Archives of Endocrinology and Metabolism Low-level laser therapy resistance training diabetes mellitus bone |
| title | Low-level laser therapy associated to a resistance training protocol on bone tissue in diabetic rats |
| title_full | Low-level laser therapy associated to a resistance training protocol on bone tissue in diabetic rats |
| title_fullStr | Low-level laser therapy associated to a resistance training protocol on bone tissue in diabetic rats |
| title_full_unstemmed | Low-level laser therapy associated to a resistance training protocol on bone tissue in diabetic rats |
| title_short | Low-level laser therapy associated to a resistance training protocol on bone tissue in diabetic rats |
| title_sort | low level laser therapy associated to a resistance training protocol on bone tissue in diabetic rats |
| topic | Low-level laser therapy resistance training diabetes mellitus bone |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972016000500457&lng=en&tlng=en |
| work_keys_str_mv | AT tatianelopespatrociniosilva lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats AT andremoreirafogacadesouza lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats AT raulloppigoulart lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats AT carolinafuirinipegorari lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats AT jussanrodriguesoliveira lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats AT kellyrossettifernandes lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats AT angelamariapaivamagri lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats AT rosamariarodriguespereira lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats AT danielarakiribeiro lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats AT marciareginanagaoka lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats AT anaclaudiamunizrenno lowlevellasertherapyassociatedtoaresistancetrainingprotocolonbonetissueindiabeticrats |