Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection
Abstract MicroRNAs (miRNAs) suppress gene expression and regulate biological processes. Following small RNA sequencing, shrimp hemocytes miRNAs differentially expressed in response to acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus (VPAHPND) were discovered and some...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-82881-2 |
| _version_ | 1818858519340777472 |
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| author | Pakpoom Boonchuen Phattarunda Jaree Kulwadee Somboonviwat Kunlaya Somboonwiwat |
| author_facet | Pakpoom Boonchuen Phattarunda Jaree Kulwadee Somboonviwat Kunlaya Somboonwiwat |
| author_sort | Pakpoom Boonchuen |
| collection | DOAJ |
| description | Abstract MicroRNAs (miRNAs) suppress gene expression and regulate biological processes. Following small RNA sequencing, shrimp hemocytes miRNAs differentially expressed in response to acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus (VPAHPND) were discovered and some were confirmed by qRT-PCR. VPAHPND-responsive miRNAs were predicted to target several genes in various immune pathways. Among them, lva-miR-4850 is of interest because its predicted target mRNAs are two important genes of the proPO system; proPO2 (PO2) and proPO activating factor 2 (PPAF2). The expression of lva-miR-4850 was significantly decreased after VPAHPND infection, whereas those of the target mRNAs, PO2 and PPAF2, and PO activity were significantly upregulated. Introducing the lva-miR-4850 mimic into VPAHPND-infected shrimps caused a reduction in the PO2 and PPAF2 transcript levels and the PO activity, but significantly increased the number of bacteria in the VPAHPND targeted tissues. This result inferred that lva-miR-4850 plays a crucial role in regulating the proPO system via suppressing expression of PPAF2 and PO2. To fight against VPAHPND infection, shrimp downregulated lva-miR-4850 expression resulted in proPO activation. |
| first_indexed | 2024-12-19T08:57:35Z |
| format | Article |
| id | doaj.art-35592cce20db413f952bf03cab7e69fc |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T08:57:35Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-35592cce20db413f952bf03cab7e69fc2022-12-21T20:28:34ZengNature PortfolioScientific Reports2045-23222021-02-0111111210.1038/s41598-021-82881-2Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infectionPakpoom Boonchuen0Phattarunda Jaree1Kulwadee Somboonviwat2Kunlaya Somboonwiwat3Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn UniversityCenter of Applied Shrimp Research and Innovation, Institute of Molecular Biosciences, Mahidol UniversityFaculty of Engineering at Sriracha, Kasetsart University Sriracha CampusCenter of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn UniversityAbstract MicroRNAs (miRNAs) suppress gene expression and regulate biological processes. Following small RNA sequencing, shrimp hemocytes miRNAs differentially expressed in response to acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus (VPAHPND) were discovered and some were confirmed by qRT-PCR. VPAHPND-responsive miRNAs were predicted to target several genes in various immune pathways. Among them, lva-miR-4850 is of interest because its predicted target mRNAs are two important genes of the proPO system; proPO2 (PO2) and proPO activating factor 2 (PPAF2). The expression of lva-miR-4850 was significantly decreased after VPAHPND infection, whereas those of the target mRNAs, PO2 and PPAF2, and PO activity were significantly upregulated. Introducing the lva-miR-4850 mimic into VPAHPND-infected shrimps caused a reduction in the PO2 and PPAF2 transcript levels and the PO activity, but significantly increased the number of bacteria in the VPAHPND targeted tissues. This result inferred that lva-miR-4850 plays a crucial role in regulating the proPO system via suppressing expression of PPAF2 and PO2. To fight against VPAHPND infection, shrimp downregulated lva-miR-4850 expression resulted in proPO activation.https://doi.org/10.1038/s41598-021-82881-2 |
| spellingShingle | Pakpoom Boonchuen Phattarunda Jaree Kulwadee Somboonviwat Kunlaya Somboonwiwat Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection Scientific Reports |
| title | Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection |
| title_full | Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection |
| title_fullStr | Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection |
| title_full_unstemmed | Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection |
| title_short | Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection |
| title_sort | regulation of shrimp prophenoloxidase activating system by lva mir 4850 during bacterial infection |
| url | https://doi.org/10.1038/s41598-021-82881-2 |
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