QTL mapping by whole genome re-sequencing and analysis of candidate genes for salt tolerance in linseed (Linum usitatissmum L.)
Soil salinization is detrimental to the growth and development of flax and ultimately leads to a decrease in yield. However, the molecular mechanism of linseed response to salt stress is still unclear. In this study, a salt-tolerant (ST) linseed variety STS and a salt-sensitive (SS) variety DYM were...
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KeAi Communications Co., Ltd.
2022-05-01
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| Series: | Oil Crop Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2096242822000185 |
| _version_ | 1818555526436356096 |
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| author | Wei Zhao Yanping Zhang Jianping Zhang Yanni Qi Limin Wang Zhao Dang Yaping Xie Wenjuan Li Li Zhao |
| author_facet | Wei Zhao Yanping Zhang Jianping Zhang Yanni Qi Limin Wang Zhao Dang Yaping Xie Wenjuan Li Li Zhao |
| author_sort | Wei Zhao |
| collection | DOAJ |
| description | Soil salinization is detrimental to the growth and development of flax and ultimately leads to a decrease in yield. However, the molecular mechanism of linseed response to salt stress is still unclear. In this study, a salt-tolerant (ST) linseed variety STS and a salt-sensitive (SS) variety DYM were selected as experiment materials. Bulk segregation analysis and whole-genome resequencing technologies were performed to map salt tolerance quantitative trait loci (QTL). A total of 38,625 QTL loci were identified. Fifteen genes (which were not annotated in the reference genome) were identified within a 2.597 Mb region in chromosome 1. Two salt tolerance candidate genes Lus.o.m.scaffold91.141 and Lus.o.m. Scaffold1.14 encoding WD40 and cytochrome P450 were identified by predicting protein functions. Previous studies showed that WD40 and cytochrome P450 could significantly improve plant salt stress tolerance. In this paper, results showed that Lus.o.m.scaffold91.141 and Lus.o.m. Scaffold1.14 might be involved in response to salt stress in lineseed. The fine mapping and functional analysis of these genes provide a molecular breeding basis for the genetic improvement of high salt-tolerant linseed varieties. |
| first_indexed | 2024-12-12T09:54:36Z |
| format | Article |
| id | doaj.art-355d83a0c13c4202b05162e46b4ab570 |
| institution | Directory Open Access Journal |
| issn | 2096-2428 |
| language | English |
| last_indexed | 2024-12-12T09:54:36Z |
| publishDate | 2022-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Oil Crop Science |
| spelling | doaj.art-355d83a0c13c4202b05162e46b4ab5702022-12-22T00:28:10ZengKeAi Communications Co., Ltd.Oil Crop Science2096-24282022-05-01728085QTL mapping by whole genome re-sequencing and analysis of candidate genes for salt tolerance in linseed (Linum usitatissmum L.)Wei Zhao0Yanping Zhang1Jianping Zhang2Yanni Qi3Limin Wang4Zhao Dang5Yaping Xie6Wenjuan Li7Li Zhao8Crop Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, ChinaGansu Provincial Academy of Agricultural Sciences Institute of Biotechnology, Lanzhou, 730070, ChinaCrop Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, ChinaCrop Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, ChinaCrop Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, ChinaCrop Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, ChinaCrop Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, ChinaCrop Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, ChinaCrop Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, China; Corresponding author.Soil salinization is detrimental to the growth and development of flax and ultimately leads to a decrease in yield. However, the molecular mechanism of linseed response to salt stress is still unclear. In this study, a salt-tolerant (ST) linseed variety STS and a salt-sensitive (SS) variety DYM were selected as experiment materials. Bulk segregation analysis and whole-genome resequencing technologies were performed to map salt tolerance quantitative trait loci (QTL). A total of 38,625 QTL loci were identified. Fifteen genes (which were not annotated in the reference genome) were identified within a 2.597 Mb region in chromosome 1. Two salt tolerance candidate genes Lus.o.m.scaffold91.141 and Lus.o.m. Scaffold1.14 encoding WD40 and cytochrome P450 were identified by predicting protein functions. Previous studies showed that WD40 and cytochrome P450 could significantly improve plant salt stress tolerance. In this paper, results showed that Lus.o.m.scaffold91.141 and Lus.o.m. Scaffold1.14 might be involved in response to salt stress in lineseed. The fine mapping and functional analysis of these genes provide a molecular breeding basis for the genetic improvement of high salt-tolerant linseed varieties.http://www.sciencedirect.com/science/article/pii/S2096242822000185LinseedSalt-tolerantWhole-genome re-sequencingQTLCandidate genes |
| spellingShingle | Wei Zhao Yanping Zhang Jianping Zhang Yanni Qi Limin Wang Zhao Dang Yaping Xie Wenjuan Li Li Zhao QTL mapping by whole genome re-sequencing and analysis of candidate genes for salt tolerance in linseed (Linum usitatissmum L.) Oil Crop Science Linseed Salt-tolerant Whole-genome re-sequencing QTL Candidate genes |
| title | QTL mapping by whole genome re-sequencing and analysis of candidate genes for salt tolerance in linseed (Linum usitatissmum L.) |
| title_full | QTL mapping by whole genome re-sequencing and analysis of candidate genes for salt tolerance in linseed (Linum usitatissmum L.) |
| title_fullStr | QTL mapping by whole genome re-sequencing and analysis of candidate genes for salt tolerance in linseed (Linum usitatissmum L.) |
| title_full_unstemmed | QTL mapping by whole genome re-sequencing and analysis of candidate genes for salt tolerance in linseed (Linum usitatissmum L.) |
| title_short | QTL mapping by whole genome re-sequencing and analysis of candidate genes for salt tolerance in linseed (Linum usitatissmum L.) |
| title_sort | qtl mapping by whole genome re sequencing and analysis of candidate genes for salt tolerance in linseed linum usitatissmum l |
| topic | Linseed Salt-tolerant Whole-genome re-sequencing QTL Candidate genes |
| url | http://www.sciencedirect.com/science/article/pii/S2096242822000185 |
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