Vitamin C Content in Daphnia sp. Enriched with L-ascorbic Acid Ethyl Cellulose at Different Enrichment Period
<p><em>Daphnia</em> with a density of 10,000 ind./l medium were enriched with either 0.1, 0.5 or 1 g l-ascorbic acid ethyl cellulose (VC) per l culture medium. In this triplicate experiment, <em>Daphnia</em> were enriched with VC in a 12 litre aquaria for 0, 3, 6 or 9 h...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Asosiasi Sains Akuakultur Indonesia
2008-01-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | http://journal.ipb.ac.id/index.php/jai/article/view/4033 |
| Summary: | <p><em>Daphnia</em> with a density of 10,000 ind./l medium were enriched with either 0.1, 0.5 or 1 g l-ascorbic acid ethyl cellulose (VC) per l culture medium. In this triplicate experiment, <em>Daphnia</em> were enriched with VC in a 12 litre aquaria for 0, 3, 6 or 9 h. Results showed that the maximum VC content in <em>Daphnia</em> enriched with 0.1, 0.5 and 1 g VC/l medium was 347, 471 and 482 mg VC/g <em>Daphnia</em> (dry basis), respectively. This VC content was obtained after 5 h, 5 h 12 min and 5 h 18 min of enrichment period. During 9 h enrichment period, the survival rate of <em>Daphnia</em> enriched with 0.1, 0.5 and 1 g VC/l medium was decrease linearly from 100% to 55.6%, 50.1% and 47.9%, respectively.</p> <p>Keywords: <em>Daphnia</em>, vitamin C, enrichment</p> <p> </p> <p>ABSTRAK</p> <p>Dalam penelitian ini, <em>Daphnia</em> dengan padat tebar 10.000 ekor/l diperkaya dengan vitamin C (VC) pada tiga dosis yang berbeda, yaitu dosis 0,1, 0,5 dan 1 g VC/l media untuk masing-masing penelitian 1, 2 dan 3. <em>Daphnia</em> diperkaya dalam wadah volume 12 liter selama 0, 3, 6 dan 9 jam. Masing-masing perlakuan diulang sebanyak 3 kali ulangan. Hasil analisa respon menunjukkan bahwa waktu pengkayaan yang optimum untuk masing-masing dosis pengkayaan 0,1, 0,5 dan 1 g VC/l media secara berturut-turut adalah 5 jam, 5 jam 12 menit dan 5 jam 18 menit dengan kadar VC masing-masing sebesar 347, 471 dan 482 mg VC/g <em>Daphnia</em>. Tingkat kelangsungan hidup <em>Daphnia</em> sp. menunjukkan terjadinya penurunan secara linier dengan semakin bertambahnya waktu pengkayaan. Tingkat kelangsungan hidup <em>Daphnia</em> sp. setelah 9 jam masa pengkayaan ialah sebesar 55,63%, 50,12% dan 47,92% untuk dosis 0,1, 0,5 dan 1 g VC/l media. </p> <p>Kata kunci: <em>Daphnia</em>, vitamin C, pengkayaan</p> |
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| ISSN: | 1412-5269 2354-6700 |