The search for conjugative transposon in rumen bacterium Prevotella bryantii B14
Only few plasmids and bacteriophages have been described to date in ruminal prevotella strains, therefore it appears plausible that the genetic exchange in these organisms must exploit other routes. Large conjugative transposons make possible the gene exchange process in bacteria from the genus Bact...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2009-12-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/14883 |
| _version_ | 1826532345519276032 |
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| author | Gregor GORENC Tomaž ACCETTO Gorazd AVGUŠTIN |
| author_facet | Gregor GORENC Tomaž ACCETTO Gorazd AVGUŠTIN |
| author_sort | Gregor GORENC |
| collection | DOAJ |
| description | Only few plasmids and bacteriophages have been described to date in ruminal prevotella strains, therefore it appears plausible that the genetic exchange in these organisms must exploit other routes. Large conjugative transposons make possible the gene exchange process in bacteria from the genus Bacteroides, the phylogenetic relatives of ruminal prevotellas. The access to fully or partially finished genome sequences of Bacteroides and Prevotella representatives made possible the search for conserved regions within putative conjugative transposons. Multiple sequence alignment of known and putative conjugative transposon gene sequences of Bacteroides thetaiotaomicron, Prevotella intermedia, Bacteroides fragilis and Tanerella sp. was used to locate partially conserved regions within most preserved conjugative transposition genes, traG, and to construct appropriate degenerated oligonucleotide primers. These were used to amplify genome fragments from ruminal prevotella strains. Sequence analysis of the subcloned PCR products revealed the presence of a hypothetical gene in the genome of Prevotella bryantii B14, similar to the ORF BF2880 from B. fragilis YCH46, which is a part of a large conjugative transposon. Inverse PCRs were designed and performed to confirm the initial findings. A partial map of P. bryantii B14 putative conjugative transposon region was constructed, indicating an intergeneric horizontal gene transfer. |
| first_indexed | 2024-03-08T23:23:33Z |
| format | Article |
| id | doaj.art-35712d3aa58a4a09bc2e30034384ee41 |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2025-03-14T01:49:45Z |
| publishDate | 2009-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-35712d3aa58a4a09bc2e30034384ee412025-03-12T12:21:36ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412009-12-0194214715210.14720/aas.2009.94.2.1488321275The search for conjugative transposon in rumen bacterium Prevotella bryantii B14Gregor GORENC0Tomaž ACCETTO1Gorazd AVGUŠTIN2Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaOnly few plasmids and bacteriophages have been described to date in ruminal prevotella strains, therefore it appears plausible that the genetic exchange in these organisms must exploit other routes. Large conjugative transposons make possible the gene exchange process in bacteria from the genus Bacteroides, the phylogenetic relatives of ruminal prevotellas. The access to fully or partially finished genome sequences of Bacteroides and Prevotella representatives made possible the search for conserved regions within putative conjugative transposons. Multiple sequence alignment of known and putative conjugative transposon gene sequences of Bacteroides thetaiotaomicron, Prevotella intermedia, Bacteroides fragilis and Tanerella sp. was used to locate partially conserved regions within most preserved conjugative transposition genes, traG, and to construct appropriate degenerated oligonucleotide primers. These were used to amplify genome fragments from ruminal prevotella strains. Sequence analysis of the subcloned PCR products revealed the presence of a hypothetical gene in the genome of Prevotella bryantii B14, similar to the ORF BF2880 from B. fragilis YCH46, which is a part of a large conjugative transposon. Inverse PCRs were designed and performed to confirm the initial findings. A partial map of P. bryantii B14 putative conjugative transposon region was constructed, indicating an intergeneric horizontal gene transfer.https://journals.uni-lj.si/aas/article/view/14883microbiologymolecular geneticsconjugative transposonprevotella bryantiibacteroides fragilis |
| spellingShingle | Gregor GORENC Tomaž ACCETTO Gorazd AVGUŠTIN The search for conjugative transposon in rumen bacterium Prevotella bryantii B14 Acta Agriculturae Slovenica microbiology molecular genetics conjugative transposon prevotella bryantii bacteroides fragilis |
| title | The search for conjugative transposon in rumen bacterium Prevotella bryantii B14 |
| title_full | The search for conjugative transposon in rumen bacterium Prevotella bryantii B14 |
| title_fullStr | The search for conjugative transposon in rumen bacterium Prevotella bryantii B14 |
| title_full_unstemmed | The search for conjugative transposon in rumen bacterium Prevotella bryantii B14 |
| title_short | The search for conjugative transposon in rumen bacterium Prevotella bryantii B14 |
| title_sort | search for conjugative transposon in rumen bacterium prevotella bryantii b14 |
| topic | microbiology molecular genetics conjugative transposon prevotella bryantii bacteroides fragilis |
| url | https://journals.uni-lj.si/aas/article/view/14883 |
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