Genome-Wide Analysis of the MADS-Box Gene Family in <i>Hibiscus syriacus</i> and Their Role in Floral Organ Development
<i>Hibiscus syriacus</i> belongs to the Malvaceae family, and is a plant with medicinal, edible, and greening values. MADS-box transcription factor is a large family of regulatory factors involved in a variety of biological processes in plants. Here, we performed a genome-wide characteri...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/406 |
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| author | Jie Wang Heng Ye Xiaolong Li Xue Lv Jiaqi Lou Yulu Chen Shuhan Yu Lu Zhang |
| author_facet | Jie Wang Heng Ye Xiaolong Li Xue Lv Jiaqi Lou Yulu Chen Shuhan Yu Lu Zhang |
| author_sort | Jie Wang |
| collection | DOAJ |
| description | <i>Hibiscus syriacus</i> belongs to the Malvaceae family, and is a plant with medicinal, edible, and greening values. MADS-box transcription factor is a large family of regulatory factors involved in a variety of biological processes in plants. Here, we performed a genome-wide characterization of MADS-box proteins in <i>H. syriacus</i> and investigated gene structure, phylogenetics, <i>cis</i>-acting elements, three-dimensional structure, gene expression, and protein interaction to identify candidate MADS-box genes that mediate petal developmental regulation in <i>H. syriacus</i>. A total of 163 candidate MADS-box genes were found and classified into type I (Mα, Mβ, and Mγ) and type II (MIKC and M<i>δ</i>). Analysis of <i>cis</i>-acting elements in the promoter region showed that most elements were correlated to plant hormones. The analysis of nine <i>HsMADS</i> expressions of two different <i>H. syriacus</i> cultivars showed that they were differentially expressed between two type flowers. The analysis of protein interaction networks also indicated that MADS proteins played a crucial role in floral organ identification, inflorescence and fruit development, and flowering time. This research is the first to analyze the MADS-box family of <i>H. syriacus</i> and provides an important reference for further study of the biological functions of the MADS-box, especially in flower organ development. |
| first_indexed | 2024-03-08T15:05:07Z |
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| id | doaj.art-3571a3f759694b56bf9c921b17b3ac98 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:05:07Z |
| publishDate | 2023-12-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3571a3f759694b56bf9c921b17b3ac982024-01-10T14:59:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125140610.3390/ijms25010406Genome-Wide Analysis of the MADS-Box Gene Family in <i>Hibiscus syriacus</i> and Their Role in Floral Organ DevelopmentJie Wang0Heng Ye1Xiaolong Li2Xue Lv3Jiaqi Lou4Yulu Chen5Shuhan Yu6Lu Zhang7College of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, China<i>Hibiscus syriacus</i> belongs to the Malvaceae family, and is a plant with medicinal, edible, and greening values. MADS-box transcription factor is a large family of regulatory factors involved in a variety of biological processes in plants. Here, we performed a genome-wide characterization of MADS-box proteins in <i>H. syriacus</i> and investigated gene structure, phylogenetics, <i>cis</i>-acting elements, three-dimensional structure, gene expression, and protein interaction to identify candidate MADS-box genes that mediate petal developmental regulation in <i>H. syriacus</i>. A total of 163 candidate MADS-box genes were found and classified into type I (Mα, Mβ, and Mγ) and type II (MIKC and M<i>δ</i>). Analysis of <i>cis</i>-acting elements in the promoter region showed that most elements were correlated to plant hormones. The analysis of nine <i>HsMADS</i> expressions of two different <i>H. syriacus</i> cultivars showed that they were differentially expressed between two type flowers. The analysis of protein interaction networks also indicated that MADS proteins played a crucial role in floral organ identification, inflorescence and fruit development, and flowering time. This research is the first to analyze the MADS-box family of <i>H. syriacus</i> and provides an important reference for further study of the biological functions of the MADS-box, especially in flower organ development.https://www.mdpi.com/1422-0067/25/1/406<i>H. syriacus</i>MADS-boxgenome-wide characterizationflower organ developmentexpression profiling |
| spellingShingle | Jie Wang Heng Ye Xiaolong Li Xue Lv Jiaqi Lou Yulu Chen Shuhan Yu Lu Zhang Genome-Wide Analysis of the MADS-Box Gene Family in <i>Hibiscus syriacus</i> and Their Role in Floral Organ Development International Journal of Molecular Sciences <i>H. syriacus</i> MADS-box genome-wide characterization flower organ development expression profiling |
| title | Genome-Wide Analysis of the MADS-Box Gene Family in <i>Hibiscus syriacus</i> and Their Role in Floral Organ Development |
| title_full | Genome-Wide Analysis of the MADS-Box Gene Family in <i>Hibiscus syriacus</i> and Their Role in Floral Organ Development |
| title_fullStr | Genome-Wide Analysis of the MADS-Box Gene Family in <i>Hibiscus syriacus</i> and Their Role in Floral Organ Development |
| title_full_unstemmed | Genome-Wide Analysis of the MADS-Box Gene Family in <i>Hibiscus syriacus</i> and Their Role in Floral Organ Development |
| title_short | Genome-Wide Analysis of the MADS-Box Gene Family in <i>Hibiscus syriacus</i> and Their Role in Floral Organ Development |
| title_sort | genome wide analysis of the mads box gene family in i hibiscus syriacus i and their role in floral organ development |
| topic | <i>H. syriacus</i> MADS-box genome-wide characterization flower organ development expression profiling |
| url | https://www.mdpi.com/1422-0067/25/1/406 |
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