Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type mice
Abstract Degenerative spinal disorders, including kyphotic deformity, are associated with a range of degenerative characteristics of the paraspinal musculature. It has therefore been hypothesized that paraspinal muscular dysfunction is a causative factor for degenerative spinal deformity; however, e...
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| Format: | Article |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-35506-9 |
| _version_ | 1797822862549057536 |
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| author | Alex M. Noonan Emily Buliung K. Josh Briar Diana Quinonez Cheryle A. Séguin Stephen H. M. Brown |
| author_facet | Alex M. Noonan Emily Buliung K. Josh Briar Diana Quinonez Cheryle A. Séguin Stephen H. M. Brown |
| author_sort | Alex M. Noonan |
| collection | DOAJ |
| description | Abstract Degenerative spinal disorders, including kyphotic deformity, are associated with a range of degenerative characteristics of the paraspinal musculature. It has therefore been hypothesized that paraspinal muscular dysfunction is a causative factor for degenerative spinal deformity; however, experimental studies demonstrating causative relationships are lacking. Male and female mice received either glycerol or saline injections bilaterally along the length of the paraspinal muscles at four timepoints, each separated by 2 weeks. Immediately after sacrifice, micro-CT was performed to measure spinal deformity; paraspinal muscle biopsies were taken to measure active, passive and structural properties; and lumbar spines were fixed for analysis of intervertebral disc (IVD) degeneration. Glycerol-injected mice demonstrated clear signs of paraspinal muscle degeneration and dysfunction: significantly (p < 0.01) greater collagen content, lower density, lower absolute active force, greater passive stiffness compared to saline-injected mice. Further, glycerol-injected mice exhibited spinal deformity: significantly (p < 0.01) greater kyphotic angle than saline-injected mice. Glycerol-injected mice also demonstrated a significantly (p < 0.01) greater IVD degenerative score (although mild) at the upper-most lumbar level compared to saline-injected mice. These findings provide direct evidence that combined morphological (fibrosis) and functional (actively weaker and passively stiffer) alterations to the paraspinal muscles can lead to negative changes and deformity within the thoracolumbar spine. |
| first_indexed | 2024-03-13T10:15:35Z |
| format | Article |
| id | doaj.art-357201b5751a4d58a1c647c671be8dd6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T10:15:35Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-357201b5751a4d58a1c647c671be8dd62023-05-21T11:14:51ZengNature PortfolioScientific Reports2045-23222023-05-0113111410.1038/s41598-023-35506-9Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type miceAlex M. Noonan0Emily Buliung1K. Josh Briar2Diana Quinonez3Cheryle A. Séguin4Stephen H. M. Brown5Department of Human Health and Nutritional Sciences, University of GuelphDepartment of Human Health and Nutritional Sciences, University of GuelphDepartment of Human Health and Nutritional Sciences, University of GuelphDepartment of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, Bone and Joint Institute, University of Western OntarioDepartment of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, Bone and Joint Institute, University of Western OntarioDepartment of Human Health and Nutritional Sciences, University of GuelphAbstract Degenerative spinal disorders, including kyphotic deformity, are associated with a range of degenerative characteristics of the paraspinal musculature. It has therefore been hypothesized that paraspinal muscular dysfunction is a causative factor for degenerative spinal deformity; however, experimental studies demonstrating causative relationships are lacking. Male and female mice received either glycerol or saline injections bilaterally along the length of the paraspinal muscles at four timepoints, each separated by 2 weeks. Immediately after sacrifice, micro-CT was performed to measure spinal deformity; paraspinal muscle biopsies were taken to measure active, passive and structural properties; and lumbar spines were fixed for analysis of intervertebral disc (IVD) degeneration. Glycerol-injected mice demonstrated clear signs of paraspinal muscle degeneration and dysfunction: significantly (p < 0.01) greater collagen content, lower density, lower absolute active force, greater passive stiffness compared to saline-injected mice. Further, glycerol-injected mice exhibited spinal deformity: significantly (p < 0.01) greater kyphotic angle than saline-injected mice. Glycerol-injected mice also demonstrated a significantly (p < 0.01) greater IVD degenerative score (although mild) at the upper-most lumbar level compared to saline-injected mice. These findings provide direct evidence that combined morphological (fibrosis) and functional (actively weaker and passively stiffer) alterations to the paraspinal muscles can lead to negative changes and deformity within the thoracolumbar spine.https://doi.org/10.1038/s41598-023-35506-9 |
| spellingShingle | Alex M. Noonan Emily Buliung K. Josh Briar Diana Quinonez Cheryle A. Séguin Stephen H. M. Brown Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type mice Scientific Reports |
| title | Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type mice |
| title_full | Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type mice |
| title_fullStr | Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type mice |
| title_full_unstemmed | Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type mice |
| title_short | Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type mice |
| title_sort | glycerol induced paraspinal muscle degeneration leads to hyper kyphotic spinal deformity in wild type mice |
| url | https://doi.org/10.1038/s41598-023-35506-9 |
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