Comparison of Interpretation between Pyrosequencing and Xpert MTB/RIF Assay in Multidrug-Resistant Tuberculosis
Indonesia is one of the countries with the highest multidrug-resistant tuberculosis cases in the world. Rapid molecular test using the Xpert MTB/RIF assay is one of the detection methods for MDR-TB. Early detection of MDR-TB is crucial for early initiation of treatment. However, Xpert MTB/RIF assay...
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| Format: | Article |
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Universitas Padjajaran
2020-12-01
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| Series: | Majalah Kedokteran Bandung |
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| Online Access: | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/2141 |
| _version_ | 1818695455770411008 |
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| author | Linda Choerunnisa Coriejati Rita Anna Tjandrawati Lidya Chaidir Ida Parwati |
| author_facet | Linda Choerunnisa Coriejati Rita Anna Tjandrawati Lidya Chaidir Ida Parwati |
| author_sort | Linda Choerunnisa |
| collection | DOAJ |
| description | Indonesia is one of the countries with the highest multidrug-resistant tuberculosis cases in the world. Rapid molecular test using the Xpert MTB/RIF assay is one of the detection methods for MDR-TB. Early detection of MDR-TB is crucial for early initiation of treatment. However, Xpert MTB/RIF assay only detects the rpoB gene mutations associated with Rifampicin resistance. Recently, WHO recommends the use of Pyrosequencing, a DNA sequencing method that can detect not only the rpoB gene but also katG and/or inhA gene mutations associated with Isoniazid resistance. The aims of this study were to compare the interpretation between the two methods and to determine the differences in codon mutation position detection of the rpoB gene and mutation detection of the katG and/or inhA gene. This was a cross-sectional comparative observational study on patients ≥18 years old interpreted as RR-TB patients based on Xpert MTB/RIF assay results who had not received MDR-TB drugs at Dr. Hasan Sadikin General Hospital, Bandung, Indonesia. Results showed there were 40 Rifampicin-resistant TB subjects interpreted by Xpert MTB/RIF assay while Pyrosequencing interpreted 30 MDR-TB, 9 RR-TB and one Isoniazid-resistant TB subjects in January - February 2020. The detection of rpoB gene codon mutation position between Xpert MTB/RIF assay and Pyrosequencing methods was not significantly different (p=0.389). Pyrosequencing had detected 27 katG gene mutations, 3 inhA gene mutations, one katG and inhA gene mutation. To conclude, Pyrosequencing can be used for accurate detection of Rifampicin and Isoniazid resistance in MDR-TB. |
| first_indexed | 2024-12-17T13:45:45Z |
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| issn | 0126-074X 2338-6223 |
| language | English |
| last_indexed | 2024-12-17T13:45:45Z |
| publishDate | 2020-12-01 |
| publisher | Universitas Padjajaran |
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| spelling | doaj.art-357281b0ef26492abc1113a093179e6c2022-12-21T21:46:09ZengUniversitas PadjajaranMajalah Kedokteran Bandung0126-074X2338-62232020-12-0152410.15395/mkb.v52n4.21411270Comparison of Interpretation between Pyrosequencing and Xpert MTB/RIF Assay in Multidrug-Resistant TuberculosisLinda Choerunnisa0Coriejati Rita1Anna Tjandrawati2Lidya Chaidir3Ida Parwati4Department of Clinical Pathology, Faculty of Medicine Universitas Padjadjaran / Dr. Hasan Sadikin General Hospital, Bandung, IndonesiaDepartment of Clinical Pathology, Faculty of Medicine Universitas Padjadjaran / Dr. Hasan Sadikin General Hospital, Bandung, IndonesiaDepartment of Clinical Pathology, Faculty of Medicine Universitas Padjadjaran / Dr. Hasan Sadikin General Hospital, Bandung, IndonesiaDepartment of Biomedical Sciences, Faculty of Medicine Universitas Padjadjaran, Bandung, IndonesiaDepartment of Clinical Pathology, Faculty of Medicine Universitas Padjadjaran / Dr. Hasan Sadikin General Hospital, Bandung, IndonesiaIndonesia is one of the countries with the highest multidrug-resistant tuberculosis cases in the world. Rapid molecular test using the Xpert MTB/RIF assay is one of the detection methods for MDR-TB. Early detection of MDR-TB is crucial for early initiation of treatment. However, Xpert MTB/RIF assay only detects the rpoB gene mutations associated with Rifampicin resistance. Recently, WHO recommends the use of Pyrosequencing, a DNA sequencing method that can detect not only the rpoB gene but also katG and/or inhA gene mutations associated with Isoniazid resistance. The aims of this study were to compare the interpretation between the two methods and to determine the differences in codon mutation position detection of the rpoB gene and mutation detection of the katG and/or inhA gene. This was a cross-sectional comparative observational study on patients ≥18 years old interpreted as RR-TB patients based on Xpert MTB/RIF assay results who had not received MDR-TB drugs at Dr. Hasan Sadikin General Hospital, Bandung, Indonesia. Results showed there were 40 Rifampicin-resistant TB subjects interpreted by Xpert MTB/RIF assay while Pyrosequencing interpreted 30 MDR-TB, 9 RR-TB and one Isoniazid-resistant TB subjects in January - February 2020. The detection of rpoB gene codon mutation position between Xpert MTB/RIF assay and Pyrosequencing methods was not significantly different (p=0.389). Pyrosequencing had detected 27 katG gene mutations, 3 inhA gene mutations, one katG and inhA gene mutation. To conclude, Pyrosequencing can be used for accurate detection of Rifampicin and Isoniazid resistance in MDR-TB.http://journal.fk.unpad.ac.id/index.php/mkb/article/view/2141multidrug-resistant tuberculosis, pyrosequencing, xpert mtb/rif assay |
| spellingShingle | Linda Choerunnisa Coriejati Rita Anna Tjandrawati Lidya Chaidir Ida Parwati Comparison of Interpretation between Pyrosequencing and Xpert MTB/RIF Assay in Multidrug-Resistant Tuberculosis Majalah Kedokteran Bandung multidrug-resistant tuberculosis, pyrosequencing, xpert mtb/rif assay |
| title | Comparison of Interpretation between Pyrosequencing and Xpert MTB/RIF Assay in Multidrug-Resistant Tuberculosis |
| title_full | Comparison of Interpretation between Pyrosequencing and Xpert MTB/RIF Assay in Multidrug-Resistant Tuberculosis |
| title_fullStr | Comparison of Interpretation between Pyrosequencing and Xpert MTB/RIF Assay in Multidrug-Resistant Tuberculosis |
| title_full_unstemmed | Comparison of Interpretation between Pyrosequencing and Xpert MTB/RIF Assay in Multidrug-Resistant Tuberculosis |
| title_short | Comparison of Interpretation between Pyrosequencing and Xpert MTB/RIF Assay in Multidrug-Resistant Tuberculosis |
| title_sort | comparison of interpretation between pyrosequencing and xpert mtb rif assay in multidrug resistant tuberculosis |
| topic | multidrug-resistant tuberculosis, pyrosequencing, xpert mtb/rif assay |
| url | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/2141 |
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